LOCUS       sequence-331100-       19327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19327)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 19327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU293
FEATURES             Location/Qualifiers
     source          1..19327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7726..7746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13479..13499
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    14011..14135
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(14160..14165,14166..14183,14184..14190)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             14244..14924
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(14310..14329)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             15244..15549
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     15428..15447
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(15590..15714)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(17968..17970,17971..17973,17974..18687)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(17968..17970,17971..17973,17974..18684)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(18013..18034)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(18274..18293)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     18621..18642
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(18686..18936)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(18947..18964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18947..18963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18956..18978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    19159..19183
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcaaaaatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
     6781 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
     6841 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
     6901 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
     6961 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
     7021 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
     7081 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
     7141 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
     7201 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
     7261 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
     7321 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
     7381 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
     7441 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
     7501 ctgttatatc atttttttta ttaattgtgt atatatatat gtgcatagat ctggattaca
     7561 tgattgtgat tatttacatg attttgttat ttacgtatgt atatatgtag atctggactt
     7621 tttggagttg ttgacttgat tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg
     7681 ttatgtatgt gcagcgaatt cggcgcgcca atggcttcta gcccaccgaa gaagaagcgg
     7741 aaggtcagct ggaaaatgga caagaagtac agcattggac ttgatattgg tacgaactca
     7801 gttgggtggg ccgttatcac cgatgaatac aaggtacctt cgaagaaatt taaagtgctg
     7861 ggcaacacag ataggcacag cattaagaag aacttgatcg gagctctgct ctttgactct
     7921 ggagaaaccg cggaggcgac aaggcttaaa cgtactgcga ggagaaggta cactcgcagg
     7981 aagaacagaa tctgttatct ccaagagatc tttagcaacg agatggcgaa ggtaaggatt
     8041 tttatgatat agtatgctta tgtattttgt actgaaagca tatcctgctt cattgggata
     8101 ttactgaaag catttaacta catgtaaact cacttgatga tcaataaact tgattttgca
     8161 ggttgacgac tcgttcttcc atcgcctcga ggaatccttc ctggtagagg aagataagaa
     8221 acacgagcgt caccccatct ttgggaatat tgttgacgaa gtagcctatc atgaaaagta
     8281 tccgactata taccaccttc gcaagaagct ggtggactca accgataagg cagaccttcg
     8341 gctcatatac ctggctctcg cgcacatgat aaagtttcgt ggccatttct tgatcgaagg
     8401 ggacctcaac ccggataact ccgatgtgga taaactgttc attcagctcg tccaaaccta
     8461 caatcagctg ttcgaggaga accccatcaa tgcatcaggt aacattcctt agttaccttt
     8521 cttttctttt tccatcataa gtttatagat tgtacatgct ttgagatttt tctttgcaaa
     8581 caatctcagg tgtcgacgcc aaggcaatac tgtctgccag actttcgaag tccagacggc
     8641 ttgagaatct gatcgctcaa ttgccaggcg agaagaagaa cggcttgttc gggaatctga
     8701 ttgcactgtc tctgggcctc acccctaact tcaaaagcaa ctttgacctc gccgaggacg
     8761 cgaagctgca gctgtcaaag gatacatacg atgatgatct ggacaatctg ctcgcccaaa
     8821 taggtgctct tgaaattgga actcttcttt tgttgtctaa acctatcaat ttctttgcgg
     8881 aaatttattt gaagctgtag agttaaaatt gagtctttta aacttttgta ggtgatcagt
     8941 atgccgacct gttcttggct gccaagaatc tgtcagacgc tatcttgctc agtgacattc
     9001 tgcgggtcaa cacggagata accaaagcgc cacttagcgc ctccatgatc aagaggtacg
     9061 acgagcatca ccaggatctg acccttctga aggctttggt tcgccagcaa ctccccgaga
     9121 agtacaagga gattttcttt gaccaatcga agaatggcta cgcagggtac attgatggag
     9181 gtaagttgtt acttatgatt gttttcctct ctgctacatg tattttgttg ttcatttctg
     9241 taagatataa gaattgagtt ttcctctgat gatattatta ggtgcaagtc aggaggaatt
     9301 ctacaaattc atcaagccta ttctggaaaa gatggacggt acagaggagc tgctcgttaa
     9361 attgaaccgc gaagatttgc ttcggaagca gcgtaccttc gacaatggca gcataccgca
     9421 ccagatccac ctcggtgagc tgcatgctat cttgaggagg caagaggact tctatccgtt
     9481 cctgaaagac aacagagaga agattgaaaa gatcctcacg ttccgcattc cctactatgt
     9541 aggttagtat catatgaaga aatacctagt ttcagttgat gaatgctatt ttctgacctc
     9601 agttgttctc ttttgagaat tatttctttt ctaatttgcc tgatttttct attaattcat
     9661 taggtccact cgcacgcggg aactcgcggt ttgcgtggat gacacgcaaa tccgaggaga
     9721 ctatcacgcc ttggaacttc gaagaggtcg tggacaaggg tgcgagtgca cagtccttca
     9781 tcgaaaggat gaccaacttc gataagaatc tcccaaatga gaaagtcctg cccaagcata
     9841 gtctcctgta cgaatacttc acggtctaca acgagctgac gaaggtgaaa tatgtgacgg
     9901 aggggatgcg caaaccggcc ttcctgtcag gtaaatcctg gtccacactt ttacgataaa
     9961 aacacaagat tttaaactat gaactgatca ataatcattc ctaaaagacc acacttttgt
    10021 tttgtttcta aagtaatttt tactgttata acaggtgagc agaagaaggc cattgtcgat
    10081 ctcttgttca aaaccaatcg gaaggtcact gtgaaacagc ttaaagagga ctactttaag
    10141 aagatcgaat gctttgattc tgtggaaatc agcggcgttg aggataggtt caatgcctct
    10201 cttggcacat accatgacct gttgaaaatc atcaaggaca aggacttcct tgacaacgag
    10261 gagaacgagg acatcctcga ggacatcgtg ctgactctca cgctgtttga ggacagagaa
    10321 atgatcgagg agcgccttaa gacttatgcg catctgttcg atgacaaggt catgaagcag
    10381 ttgaagagga ggagatatac aggtaagagg tcaaaaggtt tccgcaatga tccctctttt
    10441 tttgtttctc tagtttcaag aatttgggta tatgactaac ttctgagtgt tccttgatgc
    10501 atatttgtga tgagacaaat gtttgttcta tgttttaggt tggggaaggc tctccaggaa
    10561 gctcatcaac ggcatccgcg acaagcaatc cggcaagact atactggact ttctcaaatc
    10621 cgacggtttt gcgaatcgga acttcatgca gcttattcac gatgactcac tgaccttcaa
    10681 agaagatatc cagaaggccc aagtgtcagg tcagggcgat agccttcacg aacacatagc
    10741 caacctggct ggatcgccag ctataaagaa gggcatactg cagacagtga aggttgtgga
    10801 tgagctggtg aaggtaagtt ctgcatttgg ttatgctcct tgcattttag gtgttcgtcg
    10861 cacttccatt tccatgaata gctaagattt tttttctctg cattcattct tcttgcctca
    10921 gttctaactg tttgtggtat ttttgtttta attattgcta caggtcatgg gccgccataa
    10981 gccggagaac atcgtcatcg agatggcgag ggaaaaccag acgactcaga aagggcagaa
    11041 gaactcacgg gagcgcatga agcggataga ggaaggcatc aaggagcttg ggagtcagat
    11101 tctgaaagag cacccagtcg aaaatactca actccagaac gagaagctgt acctctatta
    11161 cctccagaat gggagagata tgtacgtcga ccaagagctc gacattaaca gactctccga
    11221 ctatgatgtg gatcacattg tccctcaatc tttcctgaag gacgatagta ttgacaacaa
    11281 ggtaaagcaa ctgtgtttta atcaatttct tgtcaggata tatggattat aacttaattt
    11341 ttgagaaatc tgtagtattt ggcgtgaaat gagtttgctt tttggtttct cccgtgttat
    11401 aggtccttac gcgctcagac aagaaccgcg gaaaatccga caatgtaccc agcgaggagg
    11461 ttgtgaagaa gatgaagaac tattggaggc agcttttgaa tgctaagctc ataacccaac
    11521 ggaaattcga caatctcacg aaggcagaaa ggggcggact gtctgagctc gacaaagccg
    11581 gcttcatcaa gcgccagttg gttgaaactc gtcagattac gaaacatgtg gcccagatac
    11641 tcgattcgcg tatgaatacg aagtatgatg agaatgacaa acttatcagg gaggtaaagg
    11701 taaagtttcc aactttcctt taccatatca aactaaagtt cgaaactttt tatttgatca
    11761 acttcaaggc cacccgatct ttctattcct gattaatttg tgatgaatcc atattgactt
    11821 ttgatggtta cgcaggtgat caccctcaag agcaaactgg ttagtgactt ccggaaggac
    11881 ttccagtttt acaaggttcg cgagatcaac aactaccatc atgcccatga cgcctacctg
    11941 aacgccgttg ttggcactgc tctcatcaag aagtatccga aactggagtc tgagtttgtg
    12001 tacggggatt acaaggtgta cgacgttagg aagatgatcg cgaagtcaga acaagagatc
    12061 ggcaaggcta ccgcgaaata cttcttttac tcgaatatca tgaacttctt caagacagag
    12121 atcactctgg cgaatggtga aatccggaag aggcctctga tcgagacaaa tggcgaaaca
    12181 ggtctgtctt tcctatttca tatgtttaat cctaggaatt tgatcaattg attgtatgta
    12241 tgtcgatccc aagactttct tgttcactta tatcttaact ctctctttgc tgtttcttgc
    12301 aggtgagatt gtctgggata agggcaggga ttttgcgact gtgcgtaagg ttctcagcat
    12361 gccccaagtc aacatagtca agaaaacgga ggttcaaacc ggtggtttct ccaaggagtc
    12421 cattctccct aagcgcaact ccgacaaact gattgcgagg aagaaggatt gggatccgaa
    12481 gaaatacgga ggctttgata gccctaccgt ggcatacagc gtactggtag tggccaaggt
    12541 ggagaagggc aagagcaaga aactgaaaag cgtcaaggaa ctgcttggaa ttaccataat
    12601 ggaaaggtcc tcgttcgaga agaatccgat cgacttcctc gaggctaaag gtaaaatatt
    12661 ggatgccaga cgatattctt tcttttgatt tgtaactttt tcctgtcaag gtcgataaat
    12721 tttatttttt ttggtaaaag gtcgataatt tttttttgga gccattatgt aattttccta
    12781 attaactgaa ccaaaattat acaaaccagg ttacaaagag gtgaagaaag acctcattat
    12841 caaactgccc aagtattcgc ttttcgaatt ggaaaatggc agaaaacgca tgctggcatc
    12901 tgccggagaa ctgcagaagg gcaacgagct ggcattgccc agtaagtacg tcaacttcct
    12961 gtacttggcc tcacactatg agaagctgaa ggggtcacca gaggacaacg agcagaagca
    13021 gttgtttgtc gagcagcaca agcactatct tgatgagatc atagagcaga tcagcgaatt
    13081 ttccaagcgg gtcattcttg cagacgctaa cctcgataag gtaaggactt ctcatgaata
    13141 ttagtggcag attagtgttg ttaaagtctt tggttagata atcgatgcct cctaattgtc
    13201 catgttttac tggttttcta caattaaagg tgctttccgc gtacaacaag cacagagata
    13261 agccgataag ggaacaagcg gaaaacatca tccacctgtt cacactgacc aatctgggag
    13321 ccccagcagc ctttaagtac ttcgatacca ctatcgacag aaagcgctac acatcaacca
    13381 aggaagtgtt ggacgctacc cttattcacc aatctattac agggctctat gagacaagga
    13441 tagatctgtc gcagttgggt ggtgactcta gggctgaccc aaagaagaag cgtaaagtct
    13501 gaggcgcgcc gagctccagg cctcccagct ttcgtccgta tcatcggttt cgacaacgtt
    13561 cgtcaagttc aatgcatcag tttcattgcc cacacaccag aatcctacta agtttgagta
    13621 ttatggcatt ggaaaagctg ttttcttcta tcatttgttc tgcttgtaat ttactgtgtt
    13681 ctttcagttt ttgttttcgg acatcaaaat gcaaatggat ggataagagt taataaatga
    13741 tatggtcctt ttgttcattc tcaaattatt attatctgtt gtttttactt taatgggttg
    13801 aatttaagta agaaaggaac taacagtgtg atattaaggt gcaatgttag acatataaaa
    13861 cagtctttca cctctctttg gttatgtctt gaattggttt gtttcttcac ttatctgtgt
    13921 aatcaagttt actatgagtc tatgatcaag taattatgca atcaagttaa gtacagtata
    13981 ggcttgagct ccctaggcct gttatcccta acaagtttgt acaaaaaagc tgaacgagaa
    14041 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
    14101 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
    14161 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
    14221 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
    14281 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
    14341 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
    14401 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
    14461 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
    14521 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
    14581 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
    14641 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
    14701 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
    14761 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
    14821 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
    14881 atgagtggca ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta
    14941 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
    15001 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
    15061 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
    15121 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
    15181 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
    15241 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
    15301 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
    15361 cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa
    15421 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
    15481 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
    15541 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
    15601 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
    15661 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttcgata
    15721 attccgatcc agcctaggcc cgggcctgag gacgcgtcca tggttaatta agacgtccgg
    15781 accgactagt ggatcctcta gagtcgacct gcaggcatgc aagcttcttc aagtgtatgt
    15841 aggtatagta acatgaacaa gaacagactc aagtacaaga tcgcatacga aaatggaaat
    15901 ggcaatgtca cttccacata atcaaacacg aatcctcata tcaacaaggc ctgagattct
    15961 aactagctca taacaactta gccaatagtt acttgagact accaaatgta tgtagaacta
    16021 aagactaagg gacagagagt tcgtctaaac aggtgaatct agtcgttgtt atctaataaa
    16081 caattcagcc ccaaatgcag aacacacata gagctctcta ttgattcaaa ttacgatctg
    16141 atactgataa cgtctagatt tttagggtta aagcaatcaa tcacctgacg attcaaggtg
    16201 gttggatcat gacgattcca gaaaacatca agcaagctct caaagctaca ctctttggga
    16261 tcatactgaa ctctaacaac ctcgttatgt cccgtagtgc cagtacagac atcctcgtaa
    16321 ctcggattgt gcacgatgcc atggctatac ccaacctcgg tcttggtcac accaggaact
    16381 ctctggtaag ctagctccac tccccagaaa caaccggcgc caaattgcgc gaattgctga
    16441 cctgaagacg gaacatcatc gtcgggtcct tgggcgattg cggcggaaga tgggtcagct
    16501 tgggcttgag gacgagaccc gaatccgagt ctgttgaaaa ggttgttcat tggggatttg
    16561 tatacggaga ttggtcgtcg agaggtttga gggaaaggac aaatgggttt ggctctggag
    16621 aaagagagtg cggctttaga gagagaattg agaggtttag agagagatgc ggcggcgatg
    16681 agcggaggag agacgacgag gacctgcatt atcaaagcag tgacgtggtg aaatttggaa
    16741 cttttaagag gcagatagat ttattatttg tatccatttt cttcattgtt ctagaatgtc
    16801 gcggaacaaa ttttaaaact aaatcctaaa tttttctaat tttgttgcca atagtggata
    16861 tgtgggccgt atagaaggaa tctattgaag gcccaaaccc atactgacga gcccaaaggt
    16921 tcgttttgcg ttttatgttt cggttcgatg ccaacgccac attctgagct aggcaaaaaa
    16981 caaacgtgtc tttgaataga ctcctctcgt taacacatgc agcggctgca tggtgacgcc
    17041 attaacacgt ggcctacaat tgcatgatgt ctccattgac acgtgacttc tcgtctcctt
    17101 tcttaatata tctaacaaac actcctacct cttccaaaat atatacacat ctttttgatc
    17161 aatctctcat tcaaaatctc attctctcta gtaaacaaga acaaaaaaat ggcggataca
    17221 gctagaggaa cccatcacga tatcatcggc agagaccagt acccgatgat gggccgagac
    17281 cgagaccagt accagatgtc cggacgagga tctgactact ccaagtctag gcagattgct
    17341 aaagctgcaa ctgctgtcac agctggtggt tccctccttg ttctctccag ccttaccctt
    17401 gttggaactg tcatagcttt gactgttgca acacctctgc tcgttatctt cagcccaatc
    17461 cttgtcccgg ctctcatcac agttgcactc ctcatcaccg gttttctttc ctctggaggg
    17521 tttggcattg ccgctataac cgttttctct tggatttaca agtaagcaca catttatcat
    17581 cttacttcat aattttgtgc aatatgtgca tgcatgtgtt gagccagtag ctttggatca
    17641 atttttttgg tcgaataaca aatgtaacaa taagaaattg caaattctag ggaacatttg
    17701 gttaactaaa tacgaaattt gacctagcta gcttgaatgt gtctgtgtat atcatctata
    17761 taggtaaaat gcttggtatg atacctattg attgtgaata ggtacgcaac gggagagcac
    17821 ccacagggat cagacaagtt ggacagtgca aggatgaagt tgggaagcaa agctcaggat
    17881 ctgaaagaca gagctcagta ctacggacag caacatactg gtggggaaca tgaccgtgac
    17941 cgtactcgtg gtggccagca cactactatg gtgagcaagg gcgaggagct gttcaccggg
    18001 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
    18061 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
    18121 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
    18181 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
    18241 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
    18301 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
    18361 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
    18421 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
    18481 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
    18541 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
    18601 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
    18661 ctcggcatgg acgagctgta caagtgatct agtaacatag atgaaccgcg cgcgataatt
    18721 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
    18781 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
    18841 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
    18901 atcttaagaa actttattgc caaatgtttg aacgatgaag cttggcactg gccgtcgttt
    18961 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    19021 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    19081 tgcgcagcct gaatggcgaa tgagcttgag cttggatcag attgtcgttt cccgccttca
    19141 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
    19201 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
    19261 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt
    19321 actttaa
//
