LOCUS       sequence-331116-        6531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 4 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B4
FEATURES             Location/Qualifiers
     source          1..6531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(4363..4369,4370..4387,4388..4393))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    4408..4429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4549..4566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4720..5308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4800..4819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5479..6270,6271..6339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6102..6121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6340..6444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6512..6530
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaagt tgatgtcttc cattctacaa aatagttaca ggaccaagcg
      421 agcgtgagag tccagacttt ttattttgat ttttccacat gcaacttgtt tttaatcagt
      481 gtctctgcgc ctgcaaggcc acggatgcaa ttccgggcac ggcgccaatc gccgcggcga
      541 tcagtggaat aaggaggggc aggataccgc cgcgcatgcg acggtgcgac gcgcgccgcc
      601 gccggtggtg cgcacgacgc atgccgcccg tcaggccgtg gccggccatg cccctcctac
      661 ggtgcattct tcctcggaat cccggcaccg ggaaacggag gcggcaggtg agggccatat
      721 ctgcaagaac cacaaagacc ggcttttaaa cgatgctggg gtggtagcgc gctgttggca
      781 gcaccagggt cctgcctcct tcgcgagcca ccctgcgcac ggaaatcggg gccagcacgg
      841 gctggcgacg gcgacggcgg cggcgggttc cagtggtggt tcggcgtcgg gtagttgctc
      901 gtcttctggg gcggtaggtg tagccacgat agccgggggt aggcgcaatg gaaggatgta
      961 gggcatattc gggcagtagc gcgctggcgg cgccgtactt cctcgaacgg cgcgggcgcc
     1021 ggggggctga aacgcgaaac atccacgggt ccgtttgcac ctccgtagag gtcttggacg
     1081 cggccgcagc gaccgcctgc accgcggcat ccgccaccgc tgaggcaacc ggggacgttt
     1141 gtgtctccat gccctctgtg gcggtggcaa tactggtgct actggtagtg ggtatctgaa
     1201 cgtccacggt ctgcacgccc agtcccggcg ccacctgctt gattggccgc acgcggacct
     1261 cgggctccag cccaggttcc acggtcattt tttccaagac atcttccagt cgctggcgct
     1321 tgggtaccat cagctgcacg gtgggtgcca agtcaccaga ctcgcgcttt aggccgcgct
     1381 tttcttcgga cggtgcaagc gcgggcagca cctgctgcag tgttacgggc tttaggctag
     1441 gtgttgggtt gccctcgtcc agcggcaacg ccagcatgtc cttatgccgc tttccgtagg
     1501 caaactcccc gaggcgctcg ttggcctgct caagcaggtc ctcgtcgccg tacacctcat
     1561 catacacgcg cttgtaggtg cgggtggagc gctcaccggg cgtaaagact acggtggtgc
     1621 cgggtcgcaa aacacgtttt acgcgtcgac ctttccactg tacccgtcgc ctgggcgcgg
     1681 tagcgtgcag cagttccacc tcgtcgtcaa gttcatcatc atcatctttc tttttctttt
     1741 tgacccgctt tagctttcgg ggcttgtaat cctgctcttc cttcttcggg gggccataga
     1801 tctccggcgc gatgacctgg agcatctctt ctttgatttt gcgcttggac atagcttcgt
     1861 tgcgcgccgc cgccgctgga tacatacaac agtacgagtc taagtagttt tttcttgcaa
     1921 tctagttgcg cggggggcgg gtgcgcacgg gcacgcgcag gccgctaacc gagtcgcgca
     1981 cccaatacac gttgcccctg cgaccctgag tcatagcact aatggccgcg gctgctgcgg
     2041 cggccgctcg tcgcctggac ctggggggca cagtgacaat acccgcggcc agccttcgag
     2101 cggcccgcat ggccgcccgt cggccggtgc gacgtgcgcg gttaagcagg gccgccgccg
     2161 cgcgttgggc ggcagtgccg ggtcggcggc ggtggcgacg tgctacgcgc ctccgccgtc
     2221 tcttcatttt agcatagcgc cgggctccgc gcaccacggt ctgaatggcc gcgtccactg
     2281 tggacactgg tggcggcgtg ggcgtgtagt tgcgcgcctc ctccaccacc gcgtcgatgg
     2341 cgtcatcgac ggtggtgcgc ccagtgcggc cgcgtttgtg cgcgccccag ggcgcgcggt
     2401 agtgcccgcg cacgcgcact gggtgttggt cggagcgctt cttggccccg ccaaacatct
     2461 tgcttgggaa gcgcaggccc cagcctgtgt tattgctggg cgatataagg atggacatgc
     2521 ttgctcaaaa agtgcggctc gataggacgc gcggcgagac tatgcccagg gccttgtaaa
     2581 cgtaggggca ggtgcggcgt ctggcgtcag taatggtcac tcgctggact cctccgatgc
     2641 tgttgcgcag cggtagcgtc ccgtgatctg tgagagcagg aacgttttca ctgacggtgg
     2701 tgatggtggg ggctggcggg cgcgccaaaa tctggttctc gggaaagcga ttgaacacgt
     2761 gggtcagaga ggtaaactgg cggatgagtt gggagtagac ggcctggtcg ttgtagaagc
     2821 tcttggagtg cacgggcaac agctcggcgc ccaccaccgg aaagttgctg atctggcgcg
     2881 tggagcggaa ggtcacgggg tcttgcatca tgtctggcaa cgaccagtag acctgctccg
     2941 agccgcaggt tacgtcagga gtgcaaagca gggtccatga gcggattccg gtctgagggt
     3001 cgccgtagtt gtatgcaagg taccagctgc ggtactgggt gaaggtgctg tcattgctta
     3061 ttaggttgta actgcgtttc ttgctgtcct ctgtcagggg tttgatcacc ggtttcttct
     3121 gaggcttctc gacctcgggt tgcgcagcgg gggcggcagc ttcggccgct gcttcggcct
     3181 cagcgcgctt ctcctcagcc cgtgtggcaa aggtgtcgcc gcgaatggca tgatcgttca
     3241 tgtcctccac cggctgcatt gccgcggctg ccgcgttgga gttctcttcc gcgccgctgc
     3301 cactgctgtt gctgccgcct gcgccacccc cgccctgttc ggtgtcatct ttcaagctcg
     3361 cctggtaggc gtccacatcc aacagtgcgg gaatgttacc accctccaga tcatcgtagg
     3421 tgatcctaaa gccctcctgg aagggttgcc gcttgcggat gcccaacaag ttgctcaggc
     3481 ggctgtgggt gaagtccacc ccgcatcctg gcagcaaaat gatgtctgga tggaaggctt
     3541 cgtttgtata taccccaggc atgacaagac cagtgacggg gtcaaacccc agtctgaagt
     3601 tgcgggtgtc aaactttacc ccgatgtcgc tttccagaac cccgttctgt ctgcccactt
     3661 tcaagtagtg ctccacgatc gcgttgttca taaggtctat ggtcatggtc tcggagtagt
     3721 tgccctcggg cagcgtgaac tccacccact cgtatttcag ctccacctga ttgtccttag
     3781 taggcaagcg cgacaccatc acccgcgcct taaacttatt ggtaaacatg aactcgttca
     3841 catttggcat gttggtatgc aggatggttt tcaggtcgcc gccccagtgc gaccggtcgt
     3901 caagattgat ggtctgtgtg cttcgaagat ctttctagaa gatctcctac aatattctca
     3961 gctgccatgg aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta
     4021 aaacttatat taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt
     4081 tttcttggca atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga
     4141 ccaactttat tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac
     4201 aggaatttta ttaaaaattt aaattttgaa gaaagttcag ggttaatagc atccattttt
     4261 tgctttgcaa gttcctcagc attcttaaca aaagacgtct cttttgacat gtttaaagtt
     4321 taaacctcct gtgtgaaatt attatccgct cataattcca cacattatac gagccggaag
     4381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4441 ctcactgcca attgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6481 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc c
//
