LOCUS       sequence-331117-       10052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 5 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B5
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4107..4116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(7884..7890,7891..7908,7909..7914))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    7929..7950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8070..8087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8241..8829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8321..8340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9000..9791,9792..9860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9623..9642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9861..9965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10033..10051
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaagt agaatggaag acatcaactt tgcgtctctg gccccgcgac
      421 acggctcgcg cccgttcatg ggaaactggc aagatatcgg caccagcaat atgagcggtg
      481 gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa tttcggttcc accgttaaga
      541 actatggcag caaggcctgg aacagcagca caggccagat gctgagggat aagttgaaag
      601 agcaaaattt ccaacaaaag gtggtagatg gcctggcctc tggcattagc ggggtggtgg
      661 acctggccaa ccaggcagtg caaaataaga ttaacagtaa gcttgatccc cgccctcccg
      721 tagaggagcc tccaccggcc gtggagacag tgtctccaga ggggcgtggc gaaaagcgtc
      781 cgcgccccga cagggaagaa actctggtga cgcaaataga cgagcctccc tcgtacgagg
      841 aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc gcccatggct accggagtgc
      901 tgggccagca cacacccgta acgctggacc tgcctccccc cgccgacacc cagcagaaac
      961 ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc tagccgcgcg tccctgcgcc
     1021 gcgccgccag cggtccgcga tcgttgcggc ccgtagccag tggcaactgg caaagcacac
     1081 tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg ccgacgatgc ttctgatagc
     1141 taacgtgtcg tatgtgtgtc atgtatgcgt ccatgtcgcc gccagaggag ctgctgagcc
     1201 gccgcgcgcc cgctttccaa gatggctacc ccttcgatga tgccgcagtg gtcttacatg
     1261 cacatctcgg gccaggacgc ctcggagtac ctgagccccg ggctggtgca gtttgcccgc
     1321 gccaccgaga cgtacttcag cctgaataac aagtttagaa accccacggt ggcgcctacg
     1381 cacgacgtga ccacagaccg gtcccagcgt ttgacgctgc ggttcatccc tgtggaccgt
     1441 gaggatactg cgtactcgta caaggcgcgg ttcaccctag ctgtgggtga taaccgtgtg
     1501 ctggacatgg cttccacgta ctttgacatc cgcggcgtgc tggacagggg ccctactttt
     1561 aagccctact ctggcactgc ctacaacgcc ctggctccca agggtgcccc aaatccttgc
     1621 gaatgggatg aagctgctac tgctcttgaa ataaacctag aagaagagga cgatgacaac
     1681 gaagacgaag tagacgagca agctgagcag caaaaaactc acgtatttgg gcaggcgcct
     1741 tattctggta taaatattac aaaggagggt attcaaatag gtgtcgaagg tcaaacacct
     1801 aaatatgccg ataaaacatt tcaacctgaa cctcaaatag gagaatctca gtggtacgaa
     1861 acagaaatta atcatgcagc tgggagagtc ctaaaaaaga ctaccccaat gaaaccatgt
     1921 tacggttcat atgcaaaacc cacaaatgaa aatggagggc aaggcattct tgtaaagcaa
     1981 caaaatggaa agctagaaag tcaagtggaa atgcaatttt tctcaactac tgaggcagcc
     2041 gcaggcaatg gtgataactt gactcctaaa gtggtattgt acagtgaaga tgtagatata
     2101 gaaaccccag acactcatat ttcttacatg cccactatta aggaaggtaa ctcacgagaa
     2161 ctaatgggcc aacaatctat gcccaacagg cctaattaca ttgcttttag ggacaatttt
     2221 attggtctaa tgtattacaa cagcacgggt aatatgggtg ttctggcggg ccaagcatcg
     2281 cagttgaatg ctgttgtaga tttgcaagac agaaacacag agctttcata ccagcttttg
     2341 cttgattcca ttggtgatag aaccaggtac ttttctatgt ggaatcaggc tgttgacagc
     2401 tatgatccag atgttagaat tattgaaaat catggaactg aagatgaact tccaaattac
     2461 tgctttccac tgggaggtgt gattaataca gagactctta ccaaggtaaa acctaaaaca
     2521 ggtcaggaaa atggatggga aaaagatgct acagaatttt cagataaaaa tgaaataaga
     2581 gttggaaata attttgccat ggaaatcaat ctaaatgcca acctgtggag aaatttcctg
     2641 tactccaaca tagcgctgta tttgcccgac aagctaaagt acagtccttc caacgtaaaa
     2701 atttctgata acccaaacac ctacgactac atgaacaagc gagtggtggc tcccgggcta
     2761 gtggactgct acattaacct tggagcacgc tggtcccttg actatatgga caacgtcaac
     2821 ccatttaacc accaccgcaa tgctggcctg cgctaccgct caatgttgct gggcaatggt
     2881 cgctatgtgc ccttccacat ccaggtgcct cagaagttct ttgccattaa aaacctcctt
     2941 ctcctgccgg gctcatacac ctacgagtgg aacttcagga aggatgttaa catggttctg
     3001 cagagctccc taggaaatga cctaagggtt gacggagcca gcattaagtt tgatagcatt
     3061 tgcctttacg ccaccttctt ccccatggcc cacaacaccg cctccacgct tgaggccatg
     3121 cttagaaacg acaccaacga ccagtccttt aacgactatc tctccgccgc caacatgctc
     3181 taccctatac ccgccaacgc taccaacgtg cccatatcca tcccctcccg caactgggcg
     3241 gctttccgcg gctgggcctt cacgcgcctt aagactaagg aaaccccatc actgggctcg
     3301 ggctacgacc cttattacac ctactctggc tctataccct acctagatgg aaccttttac
     3361 ctcaaccaca cctttaagaa ggtggccatt acctttgact cttctgtcag ctggcctggc
     3421 aatgaccgcc tgcttacccc caacgagttt gaaattaagc gctcagttga cggggagggt
     3481 tacaacgttg cccagtgtaa catgaccaaa gactggttcc tggtacaaat gctagctaac
     3541 tataacattg gctaccaggg cttctatatc ccagagagct acaaggaccg catgtactcc
     3601 ttctttagaa acttccagcc catgagccgt caggtggtgg atgatactaa atacaaggac
     3661 taccaacagg tgggcatcct acaccaacac aacaactctg gatttgttgg ctaccttgcc
     3721 cccaccatgc gcgaaggaca ggcctaccct gctaacttcc cctatccgct tataggcaag
     3781 accgcagttg acagcattac ccagaaaaag tttctttgcg atcgcaccct ttggcgcatc
     3841 ccattctcca gtaactttat gtccatgggc gcactcacag acctgggcca aaaccttctc
     3901 tacgccaact ccgcccacgc gctagacatg acttttgagg tggatcccat ggacgagccc
     3961 acccttcttt atgttttgtt tgaagtcttt gacgtggtcc gtgtgcacca gccgcaccgc
     4021 ggcgtcatcg aaaccgtgta cctgcgcacg cccttctcgg ccggcaacgc cacaacataa
     4081 agaagcaagc aacatcaaca acagctgccg ccatgggctc cagtgagcag gaactgaaag
     4141 ccattgtcaa agatcttggt tgtgggccat attttttggg cacctatgac aagcgctttc
     4201 caggctttgt ttctccacac aagctcgcct gcgccatagt caatacggcc ggtcgcgaga
     4261 ctgggggcgt acactggatg gcctttgcct ggaacccgca ctcaaaaaca tgctacctct
     4321 ttgagccctt tggcttttct gaccagcgac tcaagcaggt ttaccagttt gagtacgagt
     4381 cactcctgcg ccgtagcgcc attgcttctt cccccgaccg ctgtataacg ctggaaaagt
     4441 ccacccaaag cgtacagggg cccaactcgg ccgcctgtgg actattctgc tgcatgtttc
     4501 tccacgcctt tgccaactgg ccccaaactc ccatggatca caaccccacc atgaacctta
     4561 ttaccggggt acccaactcc atgctcaaca gtccccaggt acagcccacc ctgcgtcgca
     4621 accaggaaca gctctacagc ttcctggagc gccactcgcc ctacttccgc agccacagtg
     4681 cgcagattag gagcgccact tctttttgtc acttgaaaaa catgtaaaaa taatgtacta
     4741 gagacacttt caataaaggc aaatgctttt atttgtacac tctcgggtga ttatttaccc
     4801 ccacccttgc cgtctgcgcc gtttaaaaat caaaggggtt ctgccgcgca tcgctatgcg
     4861 ccactggcag ggacacgttg cgatactggt gtttagtgct ccacttaaac tcaggcacaa
     4921 ccatccgcgg cagctcggtg aagttttcac tccacaggct gcgcaccatc accaacgcgt
     4981 ttagcaggtc gggcgccgat atcttgaagt cgcagttggg gcctccgccc tgcgcgcgcg
     5041 agttgcgata cacagggttg cagcactgga acactatcag cgccgggtgg tgcacgctgg
     5101 ccagcacgct cttgtcggag atcagatccg cgtccaggtc ctccgcgttg ctcagggcga
     5161 acggagtcaa ctttggtagc tgccttccca aaaagggcgc gtgcccaggc tttgagttgc
     5221 actcgcaccg tagtggcatc aaaaggtgac cgtgcccggt ctgggcgtta ggatacagcg
     5281 cctgcataaa agccttgatc tgcttaaaag ccacctgagc ctttgcgcct tcagagaaga
     5341 acatgccgca agacttgccg gaaaactgat tggccggaca ggccgcgtcg tgcacgcagc
     5401 accttgcgtc ggtgttggag atctgcacca catttcggcc ccaccggttc ttcacgatct
     5461 tggccttgct agactgctcc ttcagcgcgc gctgcccgtt ttcgctcgtc acatccattt
     5521 caatcacgtg ctccttattt atcataatgc ttccgtgtag acacttaagc tcgccttcga
     5581 tctcagcgca gcggtgcagc cacaacgcgc agcccgtggg ctcgtgatgc ttgtaggtca
     5641 cctctgcaaa cgactgcagg tacgcctgca ggaatcgccc catcatcgtc acaaaggtct
     5701 tgttgctggt gaaggtcagc tgcaacccgc ggtgctcctc gttcagccag gtcttgcata
     5761 cggccgccag agcttccact tggtcaggca gtagtttgaa gttcgccttt agatcgttat
     5821 ccacgtggta cttgtccatc agcgcgcgcg cagcctccat gcccttctcc cacgcagaca
     5881 cgatcggcac actcagcggg ttcatcaccg taatttcact ttccgcttcg ctgggctctt
     5941 cctcttcctc ttgcgtccgc ataccacgcg ccactgggtc gtcttcattc agccgccgca
     6001 ctgtgcgctt acctcctttg ccatgcttga ttagcaccgg tgggttgctg aaacccacca
     6061 tttgtagcgc cacatcttct ctttcttcct cgctgtccac gattacctct ggtgatggcg
     6121 ggcgctcggg cttgggagaa gggcgcttct ttttcttctt gggcgcaatg gccaaatccg
     6181 ccgccgaggt cgatggccgc gggctgggtg tgcgcggcac cagcgcgtct tgtgatgagt
     6241 cttcctcgtc ctcggactcg atacgccgcc tcatccgctt ttttgggggc gcccggggag
     6301 gcggcggcga cggggacggg gacgacacgt cctccatggt tgggggacgt cgcgccgcac
     6361 cgcgtccgcg ctcgggggtg gtttcgcgct gctcctcttc ccgactggcc atttccttct
     6421 cctataggca gaaaaagatc atggagtcag tcgagaagaa ggacagccta accgccccct
     6481 ctgagttcgc caccaccgcc tccaccgatg ccgccaacgc gcctaccacc ttccccgtcg
     6541 aggcaccccc gcttgaggag gaggaagtga ttatcgagca ggacccaggt tttgtaagcg
     6601 aagacgacga ggaccgctca gtaccaacag aggataaaaa gcaagaccag gacaacgcag
     6661 aggcaaacga ggaacaagtc gggcgggggg acgaaaggca tggcgactac ctagatgtgg
     6721 gagacgacgt gctgttgaag catctgcagc gccagtgcgc cattatctgc gacgcgttgc
     6781 aagagcgcag cgatgtgccc ctcgccatag cggatgtcag ccttgcctac gaacgccacc
     6841 tattctcacc gcgcgtaccc cccaaacgcc aagaaaacgg cacatgcgag cccaacccgc
     6901 gcctcaactt ctaccccgta tttgccgtgc cagaggtgct tgccacctat cacatctttt
     6961 tccaaaactg caagataccc ctatcctgcc gtgccaaccg cagccgagcg gacaagcagc
     7021 tggccttgcg gcagggcgct gtcatacctg atatcgcctc gctcaacgaa gtgccaaaaa
     7081 tctttgaggg tcttggacgc gacgagaagc gcgcggcaaa cgctctgcaa caggaaaaca
     7141 gcgaaaatga aagtcactct ggagtgttgg tggaactcga gggtgacaac gcgcgcctag
     7201 ccgtactaaa acgcagcatc gaggtcaccc actttgccta cccggcactt aacctacccc
     7261 ccaaggtcat gagcacagtc atgagtgagc tgatcgtgcg ccgtgcgcag cccctggaga
     7321 gggatgcaaa tttgcaagaa caaacagagg agggcctacc cgcagttggc gacgagcagc
     7381 tagcgcgctg gcttcaaacg cgcgagcctg ccgacttgga ggagcgacgc aaactaatga
     7441 tgttcgaaca tctttctaga agatctccta caatattctc agctgccatg gaaaatcgat
     7501 gttcttcttt tattctctca agattttcag gctgtatatt aaaacttata ttaagaacta
     7561 tgctaaccac ctcatcagga accgttgtag gtggcgtggg ttttcttggc aatcgactct
     7621 catgaaaact acgagctaaa tattcaatat gttcctcttg accaacttta ttctgcattt
     7681 tttttgaacg aggtttagag caagcttcag gaaactgaga caggaatttt attaaaaatt
     7741 taaattttga agaaagttca gggttaatag catccatttt ttgctttgca agttcctcag
     7801 cattcttaac aaaagacgtc tcttttgaca tgtttaaagt ttaaacctcc tgtgtgaaat
     7861 tattatccgc tcataattcc acacattata cgagccggaa gcataaagtg taaagcctgg
     7921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc aattgctttc
     7981 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8641 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9961 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10021 ttaacctata aaaataggcg tatcacgagg cc
//
