LOCUS       sequence-331120-        3717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-347c-3X
FEATURES             Location/Qualifiers
     source          1..3717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(328..344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(341..363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    352..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(376..382,383..400,401..406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    421..442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(559..576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(730..1318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(810..829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1489..2280,2281..2349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2112..2131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2350..2454)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2522..2540
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2578..2600)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2700..2719
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2913..2935
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2927..2944
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2928..2944
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg tctaccgcgt tttaaccgcc
       61 actagaagcg cttactagtc tccaggcacg tgtgagaccg gcacatgaaa aaaaaaagca
      121 tgctcgagta tgaaaaaaaa aacgcatccc ggtgccgagg ccgctcaatt ggtcgtagac
      181 agctctagca ccgcttaaac gcacgtacgc gctgtctacc gcgttttaac cgccactaga
      241 agcgcttact agtctccagg cacgtgtgag accggcacat gaaaaaaaaa acagcggtac
      301 cagatctcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2461 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2521 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     2581 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     2641 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     2701 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     2761 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     2821 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2881 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     2941 cagtgaattc gagctcggta cccggggatc cggtaccgct gaaaaaaaaa acgcatcccg
     3001 gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg
     3061 ctgtctaccg cgttttaacc gccactagaa gcgcttacta gtctccaggc acgtgtgaga
     3121 ccggcacatg aaaaaaaaaa gcatgctcga gtatgaaaaa aaaaacgcat cccggtgccg
     3181 aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtct
     3241 accgcgtttt aaccgccact agaagcgctt actagtctcc aggcacgtgt gagaccggca
     3301 catgaaaaaa aaaacagcgg taccgctgaa aaaaaaaacg catcccggtg ccgaggccgc
     3361 tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg tctaccgcgt
     3421 tttaaccgcc actagaagcg cttactagtc tccaggcacg tgtgagaccg gcacatgaaa
     3481 aaaaaaagca tgctcgagta tgaaaaaaaa aacgcatccc ggtgccgagg ccgctcaatt
     3541 ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtctacc gcgttttaac
     3601 cgccactaga agcgcttact agtctccagg cacgtgtgag accggcacat gaaaaaaaaa
     3661 acagcggtac cgctgaaaaa aaaaacgcat cccggtgccg aggccgctca attggtc
//
