LOCUS       sequence-331126-        3730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-361d-3X
FEATURES             Location/Qualifiers
     source          1..3730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(182..199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(182..198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(407..426)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     526..548
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(586..604)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        672..776
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(777..845,846..1637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(995..1014)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1808..2396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2297..2316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2550..2567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2684..2705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2720..2725,2726..2743,2744..2750)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2758..2774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2763..2785
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2782..2798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2782..2798
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg
       61 gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
      121 aattgagcgg cctcggcacc gggatgcgtt ttttttttcg ttttgcagcg gtaccgaatt
      181 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      241 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      301 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      361 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      421 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      481 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
      601 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      721 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      781 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      841 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      901 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      961 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1021 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1081 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1141 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1201 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1261 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1321 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1381 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1441 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1501 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1561 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1621 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1741 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1801 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1861 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1921 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1981 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2041 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2101 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2161 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2221 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2281 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2341 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2401 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2461 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2521 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2581 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2641 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2701 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2761 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttggta
     2821 ccgcttttgg acgttttttt tttcatgtgc cggtctcaca cgtgcctgga gactagtaag
     2881 cgcttctagt ggcggttaaa acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag
     2941 agctgtctac gaccaattga gcggcctcgg caccgggatg cgtttttttt ttcatactcg
     3001 agcatgcttt tttttttcat gtgccggtct cacacgtgcc tggagactag taagcgcttc
     3061 tagtggcggt taaaacgcgg tagacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     3121 ctacgaccaa ttgagcggcc tcggcaccgg gatgcgtttt ttttttcgtt ttgcagcggt
     3181 accgcttttg gacgtttttt ttttcatgtg ccggtctcac acgtgcctgg agactagtaa
     3241 gcgcttctag tggcggttaa aacgcggtag acagcgcgta cgtgcgttta agcggtgcta
     3301 gagctgtcta cgaccaattg agcggcctcg gcaccgggat gcgttttttt tttcatactc
     3361 gagcatgctt ttttttttca tgtgccggtc tcacacgtgc ctggagacta gtaagcgctt
     3421 ctagtggcgg ttaaaacgcg gtagacagcg cgtacgtgcg tttaagcggt gctagagctg
     3481 tctacgacca attgagcggc ctcggcaccg ggatgcgttt tttttttcgt tttgcagcgg
     3541 taccgctttt ggacgttttt tttttcatgt gccggtctca cacgtgcctg gagactagta
     3601 agcgcttcta gtggcggtta aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct
     3661 agagctgtct acgaccaatt gagcggcctc ggcaccggga tgcgtttttt ttttcatact
     3721 cgagcatgct
//
