LOCUS       sequence-331142-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-171a-16X
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3144..3160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3144..3160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3157..3179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3168..3184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3192..3198,3199..3216,3217..3222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3237..3258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3375..3392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3546..4134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3626..3645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4305..5096,5097..5165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4928..4947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5166..5270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5338..5356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5394..5416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5516..5535
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5729..5738,1..13)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct gcagcctttt
       61 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
      121 taaacgcacg tacggaatca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
      181 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gattcagatc
      241 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
      301 tctagcaccg cttaaacgca cgtacggaat cagatcctgc agcctttttt ttttcacaat
      361 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
      421 cggattcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
      481 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagcctttt
      541 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
      601 taaacgcacg tacggattca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
      661 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gaatcagatc
      721 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
      781 tctagcaccg cttaaacgca cgtacggatt cagatcctgc agcctttttt ttttcacaat
      841 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
      901 cggaatcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
      961 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagcctttt
     1021 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     1081 taaacgcacg tacggaatca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
     1141 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gattcagatc
     1201 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
     1261 tctagcaccg cttaaacgca cgtacggaat cagatcctgc agcctttttt ttttcacaat
     1321 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     1381 cggattcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     1441 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagcctttt
     1501 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     1561 taaacgcacg tacggattca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
     1621 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gaatcagatc
     1681 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
     1741 tctagcaccg cttaaacgca cgtacggatt cagatcctgc agcctttttt ttttcacaat
     1801 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     1861 cggaatcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     1921 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagcctttt
     1981 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     2041 taaacgcacg tacggaatca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
     2101 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gattcagatc
     2161 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
     2221 tctagcaccg cttaaacgca cgtacggaat cagatcctgc agcctttttt ttttcacaat
     2281 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     2341 cggattcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     2401 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagcctttt
     2461 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     2521 taaacgcacg tacggattca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
     2581 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gaatcagatc
     2641 ctgcagcctt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
     2701 tctagcaccg cttaaacgca cgtacggatt cagatcctgc agcctttttt ttttcacaat
     2761 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     2821 cggaatcaga tcctgcagcc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     2881 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagcctttt
     2941 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     3001 taaacgcacg tacggaatca gatcctgcag cctttttttt ttcacaatcc cggtgccgag
     3061 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gattcagatc
     3121 tgcagaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3181 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3241 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3301 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5281 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5341 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     5401 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5461 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     5521 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5581 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     5641 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     5701 cgattaagtt gggtaacgcc agggttttcc cagtcacg
//
