LOCUS       sequence-331144-        4250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-177a-8X
FEATURES             Location/Qualifiers
     source          1..4250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2625..2642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2625..2641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2634..2656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2850..2869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2969..2991
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3029..3047)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3115..3219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3220..3288,3289..4080)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3438..3457)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc tgcagatctg
     1021 aatccgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag cggcctcggc
     1081 accgggattg tgaaaaaaaa aagcagtctg caggatctga ttccgtacgt gcgtttaagc
     1141 ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattgt gaaaaaaaaa
     1201 agcagtctgc aggatctgaa tccgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     1261 caattgagcg gcctcggcac cgggattgtg aaaaaaaaaa gcagtctgca ggatctgatt
     1321 ccgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
     1381 gggattgtga aaaaaaaaag cagtctgcag gatctgaatc cgtacgtgcg tttaagcggt
     1441 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattgtgaa aaaaaaaagc
     1501 agtctgcagg atctgattcc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa
     1561 ttgagcggcc tcggcaccgg gattgtgaaa aaaaaaagca gtctgcagga tctgaatccg
     1621 tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct cggcaccggg
     1681 attgtgaaaa aaaaaagcag tctgcaggat ctgattccgt acgtgcgttt aagcggtgct
     1741 agagctgtct acgaccaatt gagcggcctc ggcaccggga ttgtgaaaaa aaaaagcagt
     1801 ctgcaggatc tgaatccgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg
     1861 agcggcctcg gcaccgggat tgtgaaaaaa aaaagcagtc tgcaggatct gattccgtac
     1921 gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg caccgggatt
     1981 gtgaaaaaaa aaagcagtct gcaggatctg aatccgtacg tgcgtttaag cggtgctaga
     2041 gctgtctacg accaattgag cggcctcggc accgggattg tgaaaaaaaa aagcagtctg
     2101 caggatctga ttccgtacgt gcgtttaagc ggtgctagag ctgtctacga ccaattgagc
     2161 ggcctcggca ccgggattgt gaaaaaaaaa agcagtctgc aggatctgaa tccgtacgtg
     2221 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattgtg
     2281 aaaaaaaaaa gcagtctgca ggatctgatt ccgtacgtgc gtttaagcgg tgctagagct
     2341 gtctacgacc aattgagcgg cctcggcacc gggattgtga aaaaaaaaag cagtctgcag
     2401 gatctgaatc cgtacgtgcg tttaagcggt gctagagctg tctacgacca attgagcggc
     2461 ctcggcaccg ggattgtgaa aaaaaaaagc agtctgcagg atctgattcc gtacgtgcgt
     2521 ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gattgtgaaa
     2581 aaaaaaagca gtctgcagga tccccgggta ccgagctcga attcactggc cgtcgtttta
     2641 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     2701 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     2761 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2821 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2881 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2941 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3001 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3061 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3241 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3301 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3361 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3421 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3481 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3541 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3601 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3661 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3721 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3781 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3841 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3901 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3961 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4021 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4081 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4141 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4201 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
