LOCUS       sequence-331156-       10324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish Csf1b in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Hason M, Mikulasova T, Machonova O, Pombinho AR, van Ham TJ, Irion 
            U, Nusslein-Volhard C, Bartunek P, Svoboda O
  TITLE     M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via 
            distinct action of its receptors and ligands.
  JOURNAL   Blood Adv. 2022 Jan 3. pii: 483305. doi: 
            10.1182/bloodadvances.2021005459.
  PUBMED    34979548
REFERENCE   2  (bases 1 to 10324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood Adv. 
            2022 Jan 3. pii: 483305. doi: 10.1182/bloodadvances.2021005459."
COMMENT     Sequence Label: pAc-His-zfCsf1b
FEATURES             Location/Qualifiers
     source          1..10324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4273..4290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5332..6037
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5420..5792
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5449..6037)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6471..6488)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7705..7721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7705..7721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7718..7740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7729..7745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7753..7759,7760..7777,7778..7783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7798..7819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7936..7953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8107..8695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8187..8206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8866..9657,9658..9726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9489..9508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9727..9831)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9899..9917
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9955..9977)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10077..10096
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10290..10312
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10304..10321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10305..10321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcttgga
     4261 tctatggcta gccaccatca ccatcaccat ggtggatccg acatccccgg tccttgcaaa
     4321 catgccataa ctatggacca cctgcttcaa ataaaacaac tgataagtaa tcagctccgg
     4381 actggctgct caatcacata cacattcatt gagagaaaac acctgagtgt ggtgtgttat
     4441 gtgaaggctg ctttgccaag agtgcttgaa ctggtaaacg tccacttcag atacgtgcgg
     4501 ggctcaggaa gtgcgcaatc agtggtctcc ttacagaacc tcattctcaa catctactcc
     4561 cagcactgcg taccttcatt aaacgaggaa ctggaggagg accctgtagc attcgagacg
     4621 caatacaaag agtctcctgt ccaggcactt caaagagtcg aggaggttct gtccctttac
     4681 ttacacctca ttacgtccat caacacccct gttaactgga cctgcgagca agagtacagc
     4741 agcagtgacc ccccgactgt aactcaacta accaccagca cagaggctgc ggtaggatta
     4801 ttcgggcaag atcctcaggg gtcctccggc gctgatttct aactgcagat ctgatccttt
     4861 cctgggaccc ggcaagaacc aaaaactcac tctcttcaag gaaatccgta atgttaaacc
     4921 cgacacgatg aagcttgtcg ttggatggaa aggaaaagag ttctacaggg aaacttggac
     4981 ccgcttcatg gaagacagct tccccattgt taacgaccaa gaagtgatgg atgttttcct
     5041 tgttgtcaac atgcgtccca ctagacccaa ccgttgttac aaattcctgg cccaacacgc
     5101 tctgcgttgc gaccccgact atgtacctca tgacgtgatt aggatcgtcg agccttcatg
     5161 ggtgggcagc aacaacgagt accgcatcag cctggctaag aagggcggcg gctgcccaat
     5221 aatgaacctt cactctgagt acaccaactc gttcgaacag ttcatcgatc gtgtcatctg
     5281 ggagaacttc tacaagccca tcgtttacat cggtaccgac tctgctgaag aggaggaaat
     5341 tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg cacctctgtt
     5401 cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt aagcgctaga
     5461 ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt
     5521 ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct
     5581 gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa
     5641 gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa
     5701 acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg
     5761 taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc
     5821 gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat atttaacatc
     5881 gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt
     5941 tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac
     6001 gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg ggcgtttttg
     6061 ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag aaagcgatgg
     6121 tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg gtggagctga
     6181 tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg gaggcggagg
     6241 tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca acacagtcgg
     6301 cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc tgagacgagt
     6361 gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta aaggtgcagc
     6421 gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg atggtggtgg
     6481 tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg
     6541 tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc
     6601 tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg gcaattcaat
     6661 attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt cgctatcgtt
     6721 taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa gagattgtct
     6781 caagctcgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
     6841 acttcgctgt cgtcgacgta catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt
     6901 aaaatattta aaagaacatc tctgttcagc accactgtgt tgtcgtaaat gttgtttttg
     6961 ataatttgcg cttccgcagt atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg
     7021 ttcctattat tgaataaata agattgtaca gattcatatc tacgattcgt catggccacc
     7081 acaaatgcta cgctgcaaac gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact
     7141 cggtataaaa taatcaacgg gcgctttggc aaaatatcta ttttatcgca caagcccact
     7201 agcaaattgt atttgcagaa aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa
     7261 gttcaccagt taatgagcga ccacccaaat tttataaaaa tctattttaa tcacggttcc
     7321 atcaacaacc aagtgatcgt gatggactac attgactgtc ccgatttatt tgaaacacta
     7381 caaattaaag gcgagctttc gtaccaactt gttagcaata ttattagaca gctgtgtgaa
     7441 gcgctcaacg atttgcacaa gcacaatttc atacacaacg acataaaact cgaaaatgtc
     7501 ttatatttcg aagcacttga tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa
     7561 aactcactta gcgtgcacga cggcacgttg gagtatttta gtccggaaaa aattcgacac
     7621 cacaactatg cacgttcgtt tgactggtac gccgtcggcg tgttaacata caagttgcta
     7681 accggcggcc aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9841 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     9901 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9961 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10021 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10081 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10141 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10201 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10261 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10321 tgcc
//
