LOCUS       sequence-331177-        1703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  orthogonal Mango reporter output for cell-free expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Jung JK, Rasor BJ, Rybnicky GA, Silverman AD, Standeven J, Kuhn R, 
            Granito T, Ekas HM, Wang BM, Karim AS, Lucks JB, Jewett MC
  TITLE     At-Home, Cell-Free Synthetic Biology Education Modules for 
            Transcriptional Regulation and Environmental Water Quality 
            Monitoring.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):2909-2921. doi: 
            10.1021/acssynbio.3c00223. Epub 2023 Sep 12.
  PUBMED    37699423
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00223"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "2909-2921"
COMMENT     Sequence Label: pJBL7086
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     194..213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      406..453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(471..1286)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1194..1213
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1278..1297
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(1408..1703,1..293)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
       61 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      301 gcaacgcgat cccgcgaaat tcactcatta ggcaccccag gctaatacct gacactatag
      361 ggcacgtacg aaggaaggat tggtatgtgg tatattcgta cgtgcctagc ataacccctt
      421 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag ccaattctga ttagaaaaac
      481 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      541 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
      601 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
      661 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
      721 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
      781 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
      841 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
      901 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
      961 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     1021 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1081 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1141 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     1201 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cttcgagcaa
     1261 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     1321 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     1381 gacacaacgt gagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1681 ggactcaaga cgatagttac cgg
//
