LOCUS       sequence-331180-        9550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of negative control sgRNA for CRISPRi 
            knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Ma M, Ghosh S, Tavernari D, Katarkar A, Clocchiatti A, Mazzeo L, 
            Samarkina A, Epiney J, Yu YR, Ho PC, Levesque MP, Ozdemir BC, 
            Ciriello G, Dummer R, Dotto GP
  TITLE     Sustained androgen receptor signaling is a determinant of melanoma 
            cell growth potential and tumorigenesis.
  JOURNAL   J Exp Med. 2021 Feb 1;218(2). pii: 211509. doi: 
            10.1084/jem.20201137.
  PUBMED    33112375
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2021-02-1"; volume: "218(2). pii: 211509. doi"; pages:
            " 10.1084/jem.20201137"
COMMENT     Sequence Label: Lenti_CRISPRi_sgNeg2
FEATURES             Location/Qualifiers
     source          1..9550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..239
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             240..420
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    467..592
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1085..1318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1503..1547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1725..1973
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1725..1745
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1896..1915
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1996..2071
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2127..2244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2383..3561
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2614..3552
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3509..3529
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3568..3577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3583..4599
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK"
     CDS             4624..4680
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4681..4683,4684..4686,4687..5400)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4681..4683,4684..4686,4687..5397)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4726..4747)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4987..5006)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5334..5355
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5415..6003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5468..5488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5886..5897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6075..6308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6386..6507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6423..6442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6477..6496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6547..6682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6702..6721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6703..6721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6731..6748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6731..6747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6740..6762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6889..7344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6976..6995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7186..7207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7370..7474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7475..7543,7544..8335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7693..7712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8506..9094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8995..9014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9248..9265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9382..9403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9418..9423,9424..9441,9442..9448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9456..9472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9461..9483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9480..9496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9480..9496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9515..9535
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9517..9535
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
       61 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      121 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      181 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      421 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      481 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      541 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      601 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      661 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      721 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      781 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      841 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      901 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca
     1741 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     1801 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     1861 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     1921 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgtgcgat
     1981 gggggggtgg gtagcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     2041 tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg
     2101 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2161 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2221 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     2281 cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     2341 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     2401 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     2461 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     2521 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     2581 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     2641 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     2701 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     2761 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     2821 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     2881 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     2941 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     3001 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     3061 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     3121 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     3181 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     3241 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     3301 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     3361 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     3421 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     3481 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     3541 cttccatttc aggtgtcgtg acgtacggcc accatggaat tcaaaaagcc tgaactcacc
     3601 gctacctctg tcgagaagtt tctgatcgaa aagtttgaca gtgtatccga cctgatgcag
     3661 ctctccgagg gcgaagaatc tcgggctttc agcttcgatg tgggagggcg tggatatgtc
     3721 ctgcgggtga atagctgcgc cgatggtttc tacaaagatc gctatgttta tcggcacttt
     3781 gcatccgccg ctctccctat tcccgaagtg cttgacattg gggagttcag cgagagcctg
     3841 acctattgca tctcccgccg tgcacagggt gtcaccttgc aagacctgcc tgaaaccgaa
     3901 ctgcccgctg ttctccagcc cgtcgccgag gccatggatg ccatcgctgc cgccgatctt
     3961 agccagacca gcgggttcgg cccattcgga cctcaaggaa tcggtcaata cactacatgg
     4021 cgcgatttca tctgcgctat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     4081 gacaccgtca gtgcctccgt cgcccaggct ctcgatgagc tgatgctttg ggccgaggac
     4141 tgccccgaag tccggcacct cgtgcacgcc gatttcggct ccaacaatgt cctgaccgac
     4201 aatggccgca taacagccgt cattgactgg agcgaggcca tgttcgggga ttcccaatac
     4261 gaggtcgcca acatcttctt ctggaggccc tggttggctt gtatggagca gcagacccgc
     4321 tacttcgagc ggaggcatcc cgagcttgca ggatctcctc ggctccgggc ttatatgctc
     4381 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     4441 tgggctcagg gtcgctgcga cgcaatcgtc cggtccggag ccgggactgt cgggcgtaca
     4501 caaatcgccc gcagaagcgc tgccgtctgg accgatggct gtgtggaagt gctcgccgat
     4561 agtggaaaca gacgccccag cactcgtcct agggcaaagg atctgcagtc tagaggatct
     4621 ggtgccacga atttctcact actcaagcag gccggtgatg tcgaggaaaa ccctggtcct
     4681 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4741 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4801 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4861 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4921 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4981 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5041 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5101 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5161 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5221 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5281 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5341 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     5401 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5461 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5521 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5581 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5641 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5701 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5761 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5821 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5881 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5941 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6001 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6061 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6121 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6181 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6241 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6301 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6361 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6421 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6481 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6541 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6601 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6661 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6721 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6781 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6841 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6901 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6961 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7021 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7081 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7141 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7201 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7261 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7321 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7381 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7441 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7501 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7561 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7621 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7681 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7741 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7801 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7861 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7921 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7981 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8041 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8101 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8161 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8221 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8281 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8341 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8401 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8461 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8521 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8581 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8641 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8701 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8761 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8821 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8881 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8941 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9001 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9061 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9121 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9181 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9241 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9301 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9361 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9421 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9481 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9541 aaagctggag
//
