LOCUS       sequence-331185-        1186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of human talin-1 head fragment (residues 206-405) in 
            E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and 
            enterokinase cleavage site for tag removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen
            M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orlowski A, Janis 
            J, Maatta JAE, Varjosalo M, Vattulainen I, Rog T, Svergun D, Cheng 
            RH, Wu J, Hytonen VP, Wehrle-Haller B
  TITLE     The F1 loop of the talin head domain acts as a gatekeeper in 
            integrin activation and clustering.
  JOURNAL   J Cell Sci. 2020 Oct 12;133(19). pii: 133/19/jcs239202. doi: 
            10.1242/jcs.239202.
  PUBMED    33046605
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-10-12"; volume: "133(19). pii: 133/19/jcs239202. 
            doi"; pages: " 10.1242/jcs.239202"
COMMENT     Sequence Label: t206-405
FEATURES             Location/Qualifiers
     source          1..1186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     promoter        complement(756..774)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(757..774)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(818..835)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tgggggagna naaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcnnatgg gtcgggatct gtacgacgat gacgataagg atcgatggat
      121 ccgacctcga gcttcccggg accctgtaca gctgaacctc ctgtatgtgc aggcacgaga
      181 tgacatcctg aatggctccc accctgtctc ctttgacaag gcctgtgagt ttgctggctt
      241 ccaatgccag atccagtttg ggccccacaa tgagcagaag cacaaggctg gcttccttga
      301 cctgaaggac ttcctgccca aggagtatgt gaagcagaag ggagagcgta agatcttcca
      361 ggcacacaag aattgtgggc agatgagtga gattgaggcc aaggtccgct acgtgaagct
      421 agcccgttct ctcaagactt acggtgtctc cttcttcctg gtgaaggaaa aaatgaaagg
      481 gaagaacaag ctagtgccca ggcttctggg catcaccaag gagtgtgtga tgcgagtgga
      541 tgagaagacc aaggaagtga tccaggagtg gaacctcacc aacatcaaac gctgggctgc
      601 gtctcccaaa agcttcaccc tggattttgg agattaccaa gatggctatt actcagtaca
      661 gacaactgaa ggggagcaga ttgcacagct cattgccggc tacatcgata tcatcctgaa
      721 gaagaaaaaa agctaatgag tctagagggc cctattctat agtgtcacct aaatgctaaa
      781 gcttgggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
      841 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
      901 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
      961 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaaagactg
     1021 gnntttcgtt ttatctntgt ttgtcgnnga acgctctcct gagtagncaa attcgcnggn
     1081 gngantgaac nntgcnagca ncnnnncgan ggtggcgggn ngacgtngca taactgccag
     1141 natcnntnag cnannnnatc ctggacgnnn ggnccgtttt nggcng
//
