LOCUS       sequence-331192-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 6 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B6
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(6679..6685,6686..6703,6704..6709))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    6724..6745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6865..6882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7036..7624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7116..7135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7795..8586,8587..8655))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8418..8437
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8656..8760)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8828..8846
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaagc gacgcaaact aatgatggcc gcagtgctcg ttaccgtgga
      421 gcttgagtgc atgcagcggt tctttgctga cccggagatg cagcgcaagc tagaggaaac
      481 attgcactac acctttcgac agggctacgt acgccaggcc tgcaagatct ccaacgtgga
      541 gctctgcaac ctggtctcct accttggaat tttgcacgaa aaccgccttg ggcaaaacgt
      601 gcttcattcc acgctcaagg gcgaggcgcg ccgcgactac gtccgcgact gcgtttactt
      661 atttctatgc tacacctggc agacggccat gggcgtttgg cagcagtgct tggaggagtg
      721 caacctcaag gagctgcaga aactgctaaa gcaaaacttg aaggacctat ggacggcctt
      781 caacgagcgc tccgtggccg cgcacctggc ggacatcatt ttccccgaac gcctgcttaa
      841 aaccctgcaa cagggtctgc cagacttcac cagtcaaagc atgttgcaga actttaggaa
      901 ctttatccta gagcgctcag gaatcttgcc cgccacctgc tgtgcacttc ctagcgactt
      961 tgtgcccatt aagtaccgcg aatgccctcc gccgctttgg ggccactgct accttctgca
     1021 gctagccaac taccttgcct accactctga cataatggaa gacgtgagcg gtgacggtct
     1081 actggagtgt cactgtcgct gcaacctatg caccccgcac cgctccctgg tttgcaattc
     1141 gcagctgctt aacgaaagtc aaattatcgg tacctttgag ctgcagggtc cctcgcctga
     1201 cgaaaagtcc gcggctccgg ggttgaaact cactccgggg ctgtggacgt cggcttacct
     1261 tcgcaaattt gtacctgagg actaccacgc ccacgagatt aggttctacg aagaccaatc
     1321 ccgcccgcct aatgcggagc ttaccgcctg cgtcattacc cagggccaca ttcttggcca
     1381 attgcaagcc atcaacaaag cccgccaaga gtttctgcta cgaaagggac ggggggttta
     1441 cttggacccc cagtccggcg aggagctcaa cccaatcccc ccgccgccgc agccctatca
     1501 gcagcagccg cgggcccttg cttcccagga tggcacccaa aaagaagctg cagctgccgc
     1561 cgccacccac ggacgaggag gaatactggg acagtcaggc agaggaggtt ttggacgagg
     1621 aggaggagga catgatggaa gactgggaga gcctagacga ggaagcttcc gaggtcgaag
     1681 aggtgtcaga cgaaacaccg tcaccctcgg tcgcattccc ctcgccggcg ccccagaaat
     1741 cggcaaccgg ttccagcatg gctacaacct ccgctcctca ggcgccgccg gcactgcccg
     1801 ttcgccgacc caaccgtaga tgggacacca ctggaaccag ggccggtaag tccaagcagc
     1861 cgccgccgtt agcccaagag caacaacagc gccaaggcta ccgctcatgg cgcgggcaca
     1921 agaacgccat agttgcttgc ttgcaagact gtgggggcaa catctccttc gcccgccgct
     1981 ttcttctcta ccatcacggc gtggccttcc cccgtaacat cctgcattac taccgtcatc
     2041 tctacagccc atactgcacc ggcggcagcg gcagcaacag cagcggccac acagaagcaa
     2101 aggcgaccgg atagcaagac tctgacaaag cccaagaaat ccacagcggc ggcagcagca
     2161 ggaggaggag cgctgcgtct ggcgcccaac gaacccgtat cgacccgcga gcttagaaac
     2221 aggatttttc ccactctgta tgctatattt caacagagca ggggccaaga acaagagctg
     2281 aaaataaaaa acaggtctct gcgatccctc acccgcagct gcctgtatca caaaagcgaa
     2341 gatcagcttc ggcgcacgct ggaagacgcg gaggctctct tcagtaaata ctgcgcgctg
     2401 actcttaagg actagtttcg cgccctttct caaatttaag cgcgaaaact acgtcatctc
     2461 cagcggccac acccggcgcc agcacctgtt gtcagcgcca ttatgagcaa ggaaattccc
     2521 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac
     2581 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga
     2641 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt
     2701 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc
     2761 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg
     2821 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg
     2881 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc
     2941 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag
     3001 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg
     3061 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc
     3121 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac
     3181 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc
     3241 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat
     3301 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc
     3361 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt
     3421 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc
     3481 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt
     3541 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa
     3601 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa
     3661 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg
     3721 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt
     3781 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag
     3841 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg
     3901 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc
     3961 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt
     4021 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat
     4081 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga
     4141 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg
     4201 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt
     4261 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa
     4321 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta
     4381 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt
     4441 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa
     4501 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca
     4561 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag
     4621 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct
     4681 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg
     4741 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca
     4801 gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac
     4861 atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat
     4921 gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg
     4981 cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa
     5041 caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg
     5101 attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gctttttttg
     5161 tgcgtgctcc acattggctg cggtttctca catcgaagta gactgcattc cagccttcac
     5221 agtctatttg ctttacggat ttgtcaccct cacgctcatc tgcagcctca tcactgtggt
     5281 catcgccttt atccagtgca ttgactgggt ctgtgtgcgc tttgcatatc tcagacacca
     5341 tccccagtac agggacagga ctatagctga gcttcttaga attctttaat tatgaaattt
     5401 actgtgactt ttctgctgat tatttgcacc ctatctgcgt tttgttcccc gacctccaag
     5461 cctcaaagac atatatcatg cagattcact cgtatatgga atattccaag ttgctacaat
     5521 gaaaaaagcg atctttccga agcctggtta tatgcaatca tctctgttat ggtgttctgc
     5581 agtaccatct tagccctagc tatatatccc taccttgaca ttggctggaa cgcaatagat
     5641 gccatgaacc acccaacttt ccccgcgccc gctatgcttc cactgcaaca agttgttgcc
     5701 ggcggctttg tcccagccaa tcagcctcgc ccaccttctc ccacccccac tgaaatcagc
     5761 tactttaatc taacaggagg agatgactga caccctagat ctagaaatgg acggaattat
     5821 tacagagcag cgcctgctag aaagacgcag ggcagcggcc gagcaacagc gcatgaatca
     5881 agagctccaa gacatggtta acttgcacca gtgcaaaagg ggtatctttt gtctggtaaa
     5941 gcaggccaaa gtcacctacg acagtaatac caccggacac cgccttagct acaagttgcc
     6001 aaccaagcgt cagaaattgg tggtcatggt gggagaaaag cccattacca taactcagca
     6061 ctcggtagaa accgaaggct gcattcactc accttgtcaa ggacctgagg atctctgcac
     6121 ccttattaag accctgtgcg gtctcaaaga tcttattccc tttaactaat aaaaaaaaat
     6181 aataaagcat cacttactta aaatcagtta gcaaatttct gtccagttta ttcagcattc
     6241 gaacatcttt ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct
     6301 tcttttattc tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta
     6361 accacctcat caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga
     6421 aaactacgag ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt
     6481 gaacgaggtt tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat
     6541 tttgaagaaa gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc
     6601 ttaacaaaag acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattatta
     6661 tccgctcata attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     6721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc
     6781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7801 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7861 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7921 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7981 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8041 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8101 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8161 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8221 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8281 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8341 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8401 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8461 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8521 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8581 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8641 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8701 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8761 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8821 ctataaaaat aggcgtatca cgaggcc
//
