LOCUS       sequence-331193-        8039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 7 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B7
FEATURES             Location/Qualifiers
     source          1..8039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   379..481
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        complement(join(5871..5877,5878..5895,5896..5901))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    5916..5937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6057..6074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6228..6816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6308..6327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6987..7778,7779..7847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7610..7629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7848..7952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8020..8038
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaaca tcatcaataa tataccttat tttggattga agccaatatg
      421 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      481 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      541 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      601 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      661 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      721 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      781 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      841 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      901 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      961 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
     1021 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
     1081 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
     1141 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
     1201 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
     1261 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1321 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1381 caagcttaca tcggggctac gctggttagc atagctccga gtatgcgtgt cataatcagt
     1441 gtgggttctt ttgtcatggt tcctggcggg gaagtggccg cgctggtccg tgcagacctg
     1501 cacgattatg ttcagctggc cctgcgaagg gacctacggg atcgcggtat ttttgttaat
     1561 gttccgcttt tgaatcttat acaggtctgt gaggaacctg aatttttgca atcatgattc
     1621 gctgcttgag gctgaaggtg gagggcgctc tggagcagat ttttacaatg gccggactta
     1681 atattcggga tttgcttaga gatatattga gaaggtggcg agatgagaat tatttgggca
     1741 tggttgaagg tgctggaatg tttatagagg agattcaccc tgaagggttt agcctttacg
     1801 tccacttgga cgtgagggcc gtttgccttt tggaagccat tgtgcaacat cttacaaatg
     1861 ccattatctg ttctttggct gtagagtttg accacgccac cggaggggag cgcgttcact
     1921 taatagatct tcattttgag gttttggata atcttttgga ataaaaaaaa aaaacatggt
     1981 tcttccagct cttcccgctc ctcccgtgtg tgactcgcag aacgaatgtg taggttggct
     2041 gggtgtggct tattctgcgg tggtggatgt tatcagggca gcggcgcatg aaggagttta
     2101 catagaaccc gaagccaggg ggcgcctgga tgctttgaga gagtggatat actacaacta
     2161 ctacacagag cgatctaagc ggcgagaccg gagacgcaga tctgtttgtc acgcccgcac
     2221 ctggttttgc ttcaggaaat atgactacgt ccggcgttcc atttggcatg acactacgac
     2281 caacacgatc tcggttgtct cggcgcactc cgtacagtag ggatcgtcta cctccttttg
     2341 agacagaaac ccgcgctacc atactggagg atcatccgct gctgcccgaa tgtaacactt
     2401 tgacaatgca caacgtgagt tacgtgcgag gtcttccctg cagtgtggga tttacgctga
     2461 ttcaggaatg ggttgttccc tgggatatgg ttctaacgcg ggaggagctt gtaatcctga
     2521 ggaagtgtat gcacgtgtgc ctgtgttgtg ccaacattga tatcatgacg agcatgatga
     2581 tccatggtta cgagtcctgg gctctccact gtcattgttc cagtcccggt tccctgcagt
     2641 gtatagccgg cgggcaggtt ttggccagct ggtttaggat ggtggtggat ggcgccatgt
     2701 ttaatcagag gtttatatgg taccgggagg tggtgaatta caacatgcca aaagaggtaa
     2761 tgtttatgtc cagcgtgttt atgaggggtc gccacttaat ctacctgcgc ttgtggtatg
     2821 atggccacgt gggttctgtg gtccccgcca tgagctttgg atacagcgcc ttgcactgtg
     2881 ggattttgaa caatattgtg gtgctgtgct gcagttactg tgctgattta agtgagatca
     2941 gggtgcgctg ctgtgcccgg aggacaaggc gccttatgct gcgggcggtg cgaatcatcg
     3001 ctgaggagac cactgccatg ttgtattcct gcaggacgga gcggcggcgg cagcagttta
     3061 ttcgcgcgct gctgcagcac caccgcccta tcctgatgca cgattatgac tctaccccca
     3121 tgtaggcgtg gacttctcct tcgccgcccg ttaagcaacc gcaagttgga cagcagcctg
     3181 tggctcagca gctggacagc gacatgaact taagtgagct gcccggggag tttattaata
     3241 tcactgatga gcgtttggct cgacaggaaa ccgtgtggaa tataacacct aagaatatgt
     3301 ctgttaccca tgatatgatg ctttttaagg ccagccgggg agaaaggact gtgtactctg
     3361 tgtgttggga gggaggtggc aggttgaata ctagggttct gtgagtttga ttaaggtacg
     3421 gtgatctgta taagctatgt ggtggtgggg ctatactact gaatgaaaaa tgacttgaaa
     3481 ttttctgcaa ttgaaaaata aacacgttga aacataacac aaacgattct ttattcttgg
     3541 gcaatgtatg aaaaagtgta agaggatgtg gcaaatattt cattaatgta gttgtggcca
     3601 gaccagtccc atgaaaatga catagagtat gcacttggag ttgtgtctcc tgtttcctgt
     3661 gtaccgttta gtgtaatggt tagtgttaca ggtttagttt tgtctccgtt taagtaaact
     3721 tgactgacaa tgttactttt ggcagtttta ccgtgagatt ttggataagc tgataggtta
     3781 ggcataaatc caacagcgtt tgtataggct gtgccttcag taagatctcc atttctaaag
     3841 ttccaatatt ctgggtccag gaaggaattg tttagtagca ctccattttc gtcaaatctt
     3901 ataataagat gagcactttg aactgttcca gatattggag ccaaactgcc tttaacagcc
     3961 aaaactgaaa ctgtagcaag tatttgactg ccacattttg ttaagaccaa agtgagttta
     4021 gcatctttct ctgcatttag tctacagtta ggagatggag ctggtgtggt ccacaaagtt
     4081 agcttatcat tatttttgtt tcctactgta atggcacctg tgctgtcaaa actaaggcca
     4141 gttcctagtt taggaaccat agccttgttt gaatcaaatt ctaggccatg gccaattttt
     4201 gttttgaggg gatttgtgtt tggtgcatta ggtgaaccaa attcaagccc atctcctgca
     4261 ttaatggcta tggctgtagc gtcaaacatc aaccccttgg cagtgcttag gttaacctca
     4321 agctttttgg aattgtttga agctgtaaac aagtaaaggc ctttgttgta gttaatatcc
     4381 aagttgtggg ctgagtttat aaaaagaggg ccctgtccta gtcttagatt tagttggttt
     4441 tgagcatcaa acggataact aacatcaagt ataaggcgtc tgttttgaga atcaatcctt
     4501 agtcctcctg ctacattaag ttgcatattg ccttgtgaat caaaacccaa ggctccagta
     4561 actttagttt gcaaggaagt attattaata gtcacacctg gaccagttgc tacggtcaaa
     4621 gtgtttaggt cgtctgttac atgcaaagga gccccgtact ttagtcctag ttttccattt
     4681 tgtgtataaa tgggctcttt caagtcaatg cccaagctac cagtggcagt agttagaggg
     4741 ggtgaggcag tgatagtaag ggtactgcta tcggtggtgg tgagggggcc tgatgtttgc
     4801 agggctagct ttccttctga cactgtgagg ggtccttggg tggcaatgct aagtttggag
     4861 tcgtgcacgg ttagcggggc ctgtgattgc atggtgagtg tgttgcccgc gaccattaga
     4921 ggtgcggcgg cagccacagt tagggcttct gaggtaactg tgaggggtgc agatatttcc
     4981 aggtttatgt ttgacttggt ttttttgaga ggtgggctca cagtggttac attttgggag
     5041 gtaaggttgc cggcctcgtc cagagagagg ccgttgccca ttttgagcgc aagcatgcca
     5101 ttggaggtaa ctagaggttc ggataggcgc aaagagagta ccccaggggg actctcttga
     5161 aacccattgg gggatacaaa gggaggagta agaaaaggca cagttggagg accggtttcc
     5221 gtgtcatatg gatacacggg gttgaaggta tcttcagacg gtcttgcgcg cttcatctgc
     5281 aacaacatga agatagtggg tgcggatgga caggaacagg aggaaactga cattccattt
     5341 agattgtgga gaaagtttgc agccaggagg aagctgcaat accagagctg ggaggagggc
     5401 aaggaggtgc tgctgaataa actggacagt tcgaagatct ttctagaaga tctcctacaa
     5461 tattctcagc tgccatggaa aatcgatgtt cttcttttat tctctcaaga ttttcaggct
     5521 gtatattaaa acttatatta agaactatgc taaccacctc atcaggaacc gttgtaggtg
     5581 gcgtgggttt tcttggcaat cgactctcat gaaaactacg agctaaatat tcaatatgtt
     5641 cctcttgacc aactttattc tgcatttttt ttgaacgagg tttagagcaa gcttcaggaa
     5701 actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat
     5761 ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt
     5821 ttaaagttta aacctcctgt gtgaaattat tatccgctca taattccaca cattatacga
     5881 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5941 gcgttgcgct cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6001 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6061 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6121 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6181 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6241 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6361 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6421 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6541 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6601 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6661 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6721 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6781 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7861 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7921 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7981 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcc
//
