LOCUS       sequence-331196-        5903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified block 6, with deletion in E3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B6-deltaE3
FEATURES             Location/Qualifiers
     source          1..5903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(3735..3741,3742..3759,3760..3765))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3780..3801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3921..3938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4092..4680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4172..4191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4851..5642,5643..5711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5474..5493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5712..5816)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5884..5902
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaagc gacgcaaact aatgatggcc gcagtgctcg ttaccgtgga
      421 gcttgagtgc atgcagcggt tctttgctga cccggagatg cagcgcaagc tagaggaaac
      481 attgcactac acctttcgac agggctacgt acgccaggcc tgcaagatct ccaacgtgga
      541 gctctgcaac ctggtctcct accttggaat tttgcacgaa aaccgccttg ggcaaaacgt
      601 gcttcattcc acgctcaagg gcgaggcgcg ccgcgactac gtccgcgact gcgtttactt
      661 atttctatgc tacacctggc agacggccat gggcgtttgg cagcagtgct tggaggagtg
      721 caacctcaag gagctgcaga aactgctaaa gcaaaacttg aaggacctat ggacggcctt
      781 caacgagcgc tccgtggccg cgcacctggc ggacatcatt ttccccgaac gcctgcttaa
      841 aaccctgcaa cagggtctgc cagacttcac cagtcaaagc atgttgcaga actttaggaa
      901 ctttatccta gagcgctcag gaatcttgcc cgccacctgc tgtgcacttc ctagcgactt
      961 tgtgcccatt aagtaccgcg aatgccctcc gccgctttgg ggccactgct accttctgca
     1021 gctagccaac taccttgcct accactctga cataatggaa gacgtgagcg gtgacggtct
     1081 actggagtgt cactgtcgct gcaacctatg caccccgcac cgctccctgg tttgcaattc
     1141 gcagctgctt aacgaaagtc aaattatcgg tacctttgag ctgcagggtc cctcgcctga
     1201 cgaaaagtcc gcggctccgg ggttgaaact cactccgggg ctgtggacgt cggcttacct
     1261 tcgcaaattt gtacatgagg actaccacgc ccacgagatt aggttctacg aagaccaatc
     1321 ccgcccgcct aatgcggagc ttaccgcctg cgtcattacc cagggccaca ttcttggcca
     1381 attgcaagcc atcaacaaag cccgccaaga gtttctgcta cgaaagggac ggggggttta
     1441 cttggacccc cagtccggcg aggagctcaa cccaatcccc ccgccgccgc agccctatca
     1501 gcagcagccg cgggcccttg cttcccagga tggcacccaa aaagaagctg cagctgccgc
     1561 cgccacccac ggacgaggag gaatactggg acagtcaggc agaggaggtt ttggacgagg
     1621 aggaggagga catgatggaa gactgggaga gcctagacga ggaagcttcc gaggtcgaag
     1681 aggtgtcaga cgaaacaccg tcaccctcgg tcgcattccc ctcgccggcg ccccagaaat
     1741 cggcaaccgg ttccagcatg gctacaacct ccgctcctca ggcgccgccg gcactgcccg
     1801 ttcgccgacc caaccgtaga tgggacacca ctggaaccag ggccggtaag tccaagcagc
     1861 cgccgccgtt agcccaagag caacaacagc gccaaggcta ccgctcatgg cgcgggcaca
     1921 agaacgccat agttgcttgc ttgcaagact gtgggggcaa catctccttc gcccgccgct
     1981 ttcttctcta ccatcacggc gtggccttcc cccgtaacat cctgcattac taccgtcatc
     2041 tctacagccc atactgcacc ggcggcagcg gcagcaacag cagcggccac acagaagcaa
     2101 aggcgaccgg atagcaagac tctgacaaag cccaagaaat ccacagcggc ggcagcagca
     2161 ggaggaggag cgctgcgtct ggcgcccaac gaacccgtat cgacccgcga gcttagaaac
     2221 aggatttttc ccactctgta tgctatattt caacagagca ggggccaaga acaagagctg
     2281 aaaataaaaa acaggtctct gcgatccctc acccgcagct gcctgtatca caaaagcgaa
     2341 gatcagcttc ggcgcacgct ggaagacgcg gaggctctct tcagtaaata ctgcgcgctg
     2401 actcttaagg actagtttcg cgccctttct caaatttaag cgcgaaaact acgtcatctc
     2461 cagcggccac acccggcgcc agcacctgtt gtcagcgcca ttatgagcaa ggaaattccc
     2521 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac
     2581 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga
     2641 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt
     2701 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc
     2761 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg
     2821 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg
     2881 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc
     2941 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag
     3001 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg
     3061 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc
     3121 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac
     3181 gactgaatcg gatccggtct caaagatctt attcccttta actaataaaa aaaaataata
     3241 aagcatcact tacttaaaat cagttagcaa atttctgtcc agtttattca gcattcgaac
     3301 atctttctag aagatctcct acaatattct cagctgccat ggaaaatcga tgttcttctt
     3361 ttattctctc aagattttca ggctgtatat taaaacttat attaagaact atgctaacca
     3421 cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc tcatgaaaac
     3481 tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt ttttttgaac
     3541 gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat ttaaattttg
     3601 aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca gcattcttaa
     3661 caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa ttattatccg
     3721 ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3781 tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt ccagtcggga
     3841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5821 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5881 aaaaataggc gtatcacgag gcc
//
