LOCUS       sequence-331197-        5456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified block 7, with deletion in E4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B7-deltaE4
FEATURES             Location/Qualifiers
     source          1..5456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   379..481
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        complement(join(3288..3294,3295..3312,3313..3318))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3333..3354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3474..3491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3645..4233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3725..3744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4404..5195,5196..5264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5027..5046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5265..5369)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5437..5455
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaaca tcatcaataa tataccttat tttggattga agccaatatg
      421 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      481 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      541 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      601 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      661 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      721 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      781 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      841 tattttgcct aggcaactag tgtggggcta tactactgaa tgaaaaatga cttgaaattt
      901 tctgcaattg aaaaataaac acgttgaaac ataacacaaa cgattcttta ttcttgggca
      961 atgtatgaaa aagtgtaaga ggatgtggca aatatttcat taatgtagtt gtggccagac
     1021 cagtcccatg aaaatgacat agagtatgca cttggagttg tgtctcctgt ttcctgtgta
     1081 ccgtttagtg taatggttag tgttacaggt ttagttttgt ctccgtttaa gtaaacttga
     1141 ctgacaatgt tacttttggc agttttaccg tgagattttg gataagctga taggttaggc
     1201 ataaatccaa cagcgtttgt ataggctgtg ccttcagtaa gatctccatt tctaaagttc
     1261 caatattctg ggtccaggaa ggaattgttt agtagcactc cattttcgtc aaatcttata
     1321 ataagatgag cactttgaac tgttccagat attggagcca aactgccttt aacagccaaa
     1381 actgaaactg tagcaagtat ttgactgcca cattttgtta agaccaaagt gagtttagca
     1441 tctttctctg catttagtct acagttagga gatggagctg gtgtggtcca caaagttagc
     1501 ttatcattat ttttgtttcc tactgtaatg gcacctgtgc tgtcaaaact aaggccagtt
     1561 cctagtttag gaaccatagc cttgtttgaa tcaaattcta ggccatggcc aatttttgtt
     1621 ttgaggggat ttgtgtttgg tgcattaggt gaaccaaatt caagcccatc tcctgcatta
     1681 atggctatgg ctgtagcgtc aaacatcaac cccttggcag tgcttaggtt aacctcaagc
     1741 tttttggaat tgtttgaagc tgtaaacaag taaaggcctt tgttgtagtt aatatccaag
     1801 ttgtgggctg agtttataaa aagagggccc tgtcctagtc ttagatttag ttggttttga
     1861 gcatcaaacg gataactaac atcaagtata aggcgtctgt tttgagaatc aatccttagt
     1921 cctcctgcta cattaagttg catattgcct tgtgaatcaa aacccaaggc tccagtaact
     1981 ttagtttgca aggaagtatt attaatagtc acacctggac cagttgctac ggtcaaagtg
     2041 tttaggtcgt ctgttacatg caaaggagcc ccgtacttta gtcctagttt tccattttgt
     2101 gtataaatgg gctctttcaa gtcaatgccc aagctaccag tggcagtagt tagagggggt
     2161 gaggcagtga tagtaagggt actgctatcg gtggtggtga gggggcctga tgtttgcagg
     2221 gctagctttc cttctgacac tgtgaggggt ccttgggtgg caatgctaag tttggagtcg
     2281 tgcacggtta gcggggcctg tgattgcatg gtgagtgtgt tgcccgcgac cattagaggt
     2341 gcggcggcag ccacagttag ggcttctgag gtaactgtga ggggtgcaga tatttccagg
     2401 tttatgtttg acttggtttt tttgagaggt gggctcacag tggttacatt ttgggaggta
     2461 aggttgccgg cctcgtccag agagaggccg ttgcccattt tgagcgcaag catgccattg
     2521 gaggtaacta gaggttcgga taggcgcaaa gagagtaccc cagggggact ctcttgaaac
     2581 ccattggggg atacaaaggg aggagtaaga aaaggcacag ttggaggacc ggtttccgtg
     2641 tcatatggat acacggggtt gaaggtatct tcagacggtc ttgcgcgctt catctgcaac
     2701 aacatgaaga tagtgggtgc ggatggacag gaacaggagg aaactgacat tccatttaga
     2761 ttgtggagaa agtttgcagc caggaggaag ctgcaatacc agagctggga ggagggcaag
     2821 gaggtgctgc tgaataaact ggacagttcg aagatctttc tagaagatct cctacaatat
     2881 tctcagctgc catggaaaat cgatgttctt cttttattct ctcaagattt tcaggctgta
     2941 tattaaaact tatattaaga actatgctaa ccacctcatc aggaaccgtt gtaggtggcg
     3001 tgggttttct tggcaatcga ctctcatgaa aactacgagc taaatattca atatgttcct
     3061 cttgaccaac tttattctgc attttttttg aacgaggttt agagcaagct tcaggaaact
     3121 gagacaggaa ttttattaaa aatttaaatt ttgaagaaag ttcagggtta atagcatcca
     3181 ttttttgctt tgcaagttcc tcagcattct taacaaaaga cgtctctttt gacatgttta
     3241 aagtttaaac ctcctgtgtg aaattattat ccgctcataa ttccacacat tatacgagcc
     3301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3361 ttgcgctcac tgccaattgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3421 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3841 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4081 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4321 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4441 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4681 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5341 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5401 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcc
//
