LOCUS       sequence-33120-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Yu X, Zou J, Ye Z, Hammond H, Chen G, Tokunaga A, Mali P, Li YM, 
            Civin C, Gaiano N, Cheng L
  TITLE     Notch signaling activation in human embryonic stem cells is required
            for embryonic, but not trophoblastic, lineage commitment.
  JOURNAL   Cell Stem Cell. 2008 May 8. 2(5):461-71.
  PUBMED    18462696
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 May 8. 2(5):461-71."
COMMENT     Sequence Label: pCBFRE-(mt)-luc
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(68..264)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      82..217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    475..596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(486..505)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     540..559
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(923..944)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccattt taccaacagt accggaatgc
       61 caagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
      121 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
      181 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
      241 tggttgctga ctaattgaga tgcagatcca taaatttttt ccaagcccgt gtttacacca
      301 gatccataaa ttttttccaa gcccgtgttt acaccagatc cataaatttt ttccaagccc
      361 gtgtttacac cagatccata aattttttcc aagcccgtgt ttacaccaga tctcgagcta
      421 gcacgcgtaa gagctcggta cctcccgggt tatgttagct cagttacagt accataagat
      481 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      541 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      601 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
      661 gcaagtaaaa cctccacgta ccttaatatt acttacttat catggtagct tgggctggcg
      721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      781 atggcaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      841 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      901 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactat
//
