LOCUS       sequence-331201-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for inducible knockdown of human ARNT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, Friedel 
            RH, Zou H
  TITLE     Circadian clock regulator Bmal1 gates axon regeneration via Tet3 
            epigenetics in mouse sensory neurons.
  JOURNAL   Nat Commun. 2023 Aug 24;14(1):5165. doi: 10.1038/s41467-023-40816-7.
  PUBMED    37620297
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-24"; volume: "14(1):5165. doi"; pages: " 
            10.1038/s41467-023-40816-7"
COMMENT     Sequence Label: pLKO-Tet-On-ARNT-shRNA1
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..75
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    192..213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(228..233,234..251,252..258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    266..282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     271..293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     290..306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     290..306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     325..345
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        327..345
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        373..599
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             600..780
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    827..952
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1445..1678
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1863..1907
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2196..2290
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2207..2226
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2244..2262
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2390..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2556..3066
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2985..3003
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3075..3647
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3655..3674
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3702..3720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3731..4351
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3804..3823)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4386..4959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4553..4570)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4780..4799
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4979..5578
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4979..4998)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5475..5495
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5706..5939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6011..6132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6048..6067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6102..6121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6172..6307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6328..6346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6356..6373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6356..6372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6365..6387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6514..6969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6601..6620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6811..6832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6995..7099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7100..7168,7169..7960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7318..7337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8131..8719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8620..8639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
       61 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      121 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      181 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      241 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      301 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      361 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      421 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      481 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      541 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      601 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      661 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      721 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      781 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      841 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      901 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      961 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1021 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1081 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1141 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1201 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1261 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1321 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1381 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1441 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1501 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1561 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1621 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1681 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1741 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1801 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1861 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1921 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1981 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2041 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2101 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2161 ggtatcgatc acgagactag cctcgagcgg ccgcaatatt tgcatgtcgc tatgtgttct
     2221 gggaaatcac cataaacgtg aaatccctat cagtgataga gacttataag ttccctatca
     2281 gtgatagaga caccgggaga agtcagatgg tttatttctc gagaaataaa ccatctgact
     2341 tctcttttta attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
     2401 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2461 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2521 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
     2581 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     2641 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     2701 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     2761 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     2821 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     2881 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     2941 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     3001 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     3061 ccccaggggg atctgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
     3121 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc
     3181 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
     3241 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
     3301 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
     3361 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
     3421 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
     3481 ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct
     3541 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
     3601 cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3661 tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg
     3721 gcgaattgat atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     3781 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     3841 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     3901 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     3961 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     4021 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     4081 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     4141 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     4201 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     4261 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     4321 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttctaggg
     4381 atctgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4441 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     4501 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4561 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4621 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4681 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4741 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4801 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4861 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4921 cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat
     4981 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5041 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5101 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5161 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5221 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5281 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5341 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5401 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5461 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5521 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     5581 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc
     5641 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5701 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5761 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5821 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5881 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5941 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6001 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6061 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6121 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     6181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6301 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     6361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6481 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6841 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6901 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6961 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7021 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7081 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7141 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7201 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7261 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7321 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7381 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7441 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7501 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7561 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7621 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7681 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7741 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7801 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7861 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7921 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7981 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8041 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8101 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8161 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8221 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8281 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8341 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8401 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8461 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8521 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8581 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8641 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8701 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8761 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataac
//
