LOCUS       sequence-33121-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Yu X, Zou J, Ye Z, Hammond H, Chen G, Tokunaga A, Mali P, Li YM, 
            Civin C, Gaiano N, Cheng L
  TITLE     Notch signaling activation in human embryonic stem cells is required
            for embryonic, but not trophoblastic, lineage commitment.
  JOURNAL   Cell Stem Cell. 2008 May 8. 2(5):461-71.
  PUBMED    18462696
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 May 8. 2(5):461-71."
COMMENT     Sequence Label: pCBFRE-luc
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(69..265)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      83..218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    472..593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(483..502)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     537..556
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatt ttaccaacag taccggaatg
       61 ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      121 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      181 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      241 atggttgctg actaattgag atgcagatcc ataaattttt tcccacggcg tgttactcca
      301 gatccataaa ttttttccca cggcgtgtta ctccagatcc ataaattttt tcccacggcg
      361 tgttactcca gatccataaa ttttttccca cggcgtgtta ctccagatct cgagctagca
      421 cgcgtaagag ctcggtacct cccgggttat gttagctcag ttacagtacc ataagataca
      481 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      541 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      601 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
      661 agtaaaacct ccacgtacct taatattact tacttatcat ggtagcttgg gctggcgtaa
      721 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      781 gcaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      841 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      901 gatagggttg agtg
//
