LOCUS       sequence-331224-       10265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin resistant LexA driver vector with Mini-white CDS eye 
            marker. Contains an enhancer grammar GB20 entry point for custom 
            enhancers. Standard cut-and-paste cloning can also be used. Vector 
            uses Purple-White bacteria colony screening for identification of 
            correct cloning .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pPuroR-MCS-LexA-VP16-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..122)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(574..645,1047..1320,1395..2049,2111..2426,2630..2761,
                     2832..3446)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     rep_origin      complement(4213..4801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4293..4312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5269..6060
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6115..6570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6202..6221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6412..6433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    6693..6762
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        6790..7241
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7189..7210
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        7995..8013
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7995..8012
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8024..8041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      8849..8943
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    8950..9006
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(9068..9086)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(9249..9500,9501..9854)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             9906..10139
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDTADFEFEQMFTDALGIDEYGG"
     polyA_signal    join(10147..10265,1..16)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10171..10190)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10225..10244
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttcgc tgtcaggagg aattctagta tgtatgtaag ttaataaaac
      121 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
      181 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
      241 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
      301 tctctgtcca cagaaatatc gccgtcactt tcgccgctgc gtccgctatc tctttcgcca
      361 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
      421 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
      481 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
      541 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
      601 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
      661 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
      721 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
      781 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
      841 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
      901 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
      961 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     1021 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     1081 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     1141 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     1201 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     1261 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     1321 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     1381 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     1441 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     1501 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     1561 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     1621 ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg
     1681 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     1741 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     1801 cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     1861 ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     1921 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     1981 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     2041 cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac
     2101 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     2161 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     2221 gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     2281 atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     2341 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     2401 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     2461 cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc
     2521 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc
     2581 atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
     2641 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa
     2701 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
     2761 tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg
     2821 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
     2881 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca
     2941 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
     3001 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
     3061 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
     3121 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
     3181 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
     3241 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
     3301 ccagttcggg caaggtcatc ctggagacac ttaacttctc cgccgccgat ctgccgctgg
     3361 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
     3421 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
     3481 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
     3541 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
     3601 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
     3661 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
     3721 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
     3781 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
     3841 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
     3901 tcggctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
     3961 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
     4021 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
     4081 gtgtttgttc cttgtgtaga tgcatcgctt gacacggtta tccacagaat caggggataa
     4141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4621 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgtgaccagc
     4921 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     4981 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     5041 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     5101 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     5161 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     5221 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     5281 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     5341 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     5401 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     5461 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     5521 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     5581 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     5641 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     5701 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     5761 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     5821 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     5881 aaggatgtag ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     5941 cttgaagcta gacaggctta tcttggacaa gaagaagatc gtttggcctc gcgcgcagat
     6001 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     6061 tcactagacc aatgttacac atatatactt tagattgatt taaaacttca tgttacgcgc
     6121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccagcgc
     6361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6421 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6541 attttaacaa aatattaacg cttacaattt taacggatca gcgcagctgc ccgggtctag
     6601 agtcgacgca tgcctcctga cagcgagatg tcctaatatg gacatcctct ccggatccca
     6661 gggaggaatt ggtcgacgat gtaggtcact gtctcgaagc cgcggtgcgg gtgccagggc
     6721 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgcgc
     6781 tgtcaggagc tagaatccca aaacaaactg gttattgtgg taggtcattt gtttggcaga
     6841 aagaaaactc gagaaatttc tctggccgtt attcgttatt ctctcttttc tttttgggtc
     6901 gctccctctc tgcactaatg ctctctcact ctgtcacaca gtaaacggca tactgctctc
     6961 gttggttcga gagagcgcgc ctcgaatgtt cgcgaaaaga gcgccggagt ataaatagag
     7021 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     7081 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gtaaagtgca
     7141 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa
     7201 atctgccaag aagtaattat tgaatacaag aagagaactc tcaatactcg aatccatgtg
     7261 ggagtttatt cttgacacag atatttatga tataataact gagtaagctt aacataagga
     7321 ggaaaacaaa atgaccgagt ataagcccac ggtgcgtctg gccacccgtg atgatgtgcc
     7381 ccgtgccgtg cgaaccctgg ctgctgcctt cgccgattac ccagccaccc gtcacaccgt
     7441 ggacccagac cgtcacattg agcgtgtgac cgagctccag gagctgttcc tgacacgcgt
     7501 gggcctggat atcggcaagg tctgggtggc cgatgatgga gctgccgtgg ccgtgtggac
     7561 cacccccgag agcgtggagg ctggagccgt gttcgccgag attggacccc gtatggccga
     7621 gctgagtggc tcccgtctgg ctgcccagca gcagatggag ggcctgctgg ccccacaccg
     7681 tccaaaggag cccgtctggt tcctggccac cgtgggcgtg agccccgacc accagggcaa
     7741 gggcctgggc agtgccgtgg tgctgccagg agtggaagct gccgagcgtg ctggagtgcc
     7801 agccttcctg gaaaccagtg ccccacgcaa tctgcccttc tacgagcgtc tgggcttcac
     7861 cgtgacggcc gatgtggagg tgcccgaggg cccacgcacc tggtgtatga cccgtaagcc
     7921 aggagcctaa gcttggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     7981 tccgctgtca ggagatttag gtgacactat agacttccgc ttcctcgctc actgactcgc
     8041 tgcgctcggt cgttcggctg tcgggagaga gaccaatcca tgtgggagtt tattcttgac
     8101 acagatattt atgatataat aactgagtaa gcttaacata aggaggaaaa caaaatggcg
     8161 agcttggtta agaaagatat gtgtgttaag atgacgatgg aaggtactgt gaacggttat
     8221 cactttaagt gcgttggcga gggtgaaggc aagccgttcg agggcacgca gaacatgcgc
     8281 attcgtgtca ccgagggcgg tccgctgcct tttgcattcg acatcctggc cccgtgctgt
     8341 atgtacggct ctaagacctt cattaaacac gtgagcggta tcccggatta ctttaaagag
     8401 tcctttccag agggcttcac ttgggaacgt acccagattt ttgaggacgg tggtgttctg
     8461 accgcgcacc aagacaccag cctggaaggt aattgcctga tctataaagt gaaggttctg
     8521 ggtaccaatt tcccggcgaa tggtccggtg atgcaaaaga aaaccgcggg ttgggagccg
     8581 tgcgtcgaga tgctgtatcc gcgtgacggc gtcttgtgtg gtcagagctt gatggcgctg
     8641 aagtgcaccg atggcaatca tctgaccagc cacctgcgca cgacgtatcg tagccgtaaa
     8701 ccgagcaacg ccgttaacat gccggagttc cattttggtg accatcgcat cgaaatcctg
     8761 aaagctgagc agggcaaatt ctacgaacaa tacgaatcgg ctgtcgcacg ttacagcgat
     8821 gtgccggaaa aagcgacgta atgagcttga ctcctgttga tagatccagt aatgacctca
     8881 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     8941 aattacttca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     9001 gaattcggtc tcttcccgct tgtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     9061 catcaggcct atagtgagtc gtattatccc gagctcgccc ggggatcgag cgcagcggta
     9121 taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag ccgagcagac
     9181 gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca agacgcatac
     9241 caaaccaaat gaaggctctc acggcccgac aacaggaagt ttttgatttg atacgggatc
     9301 atatatccca aacgggtatg cctccgaccc gcgcagagat agcacagcga ctgggctttc
     9361 gatcgcctaa cgccgcggag gagcacttga aggcactggc ccgcaagggt gtcattgaaa
     9421 tcgtgtccgg tgcgagccgc ggaatccggc tgttgcagga ggaggaagag ggcctgccac
     9481 tggtgggacg cgtggccgct ggcgagccgc tgctggccca gcaacacata gagggacact
     9541 atcaggtgga cccctccttg tttaagccaa atgctgattt cctgttgcgg gtgtcgggaa
     9601 tgtccatgaa ggacatcggt attatggatg gtgacctcct cgccgtccat aagacacagg
     9661 atgtcaggaa cggccaggta gtcgttgcca ggatagacga tgaggtcact gtgaaacgcc
     9721 tcaagaagca aggcaataag gtcgagctgc tgccggagaa tagcgagttc aagccgatcg
     9781 tggtggatct gcgacagcag tcctttacta tcgagggctt ggccgtgggt gtgatccgca
     9841 acggagattg gctgagtggc ggaggttccg gtggcggcag cggcgggggt tcgggaggtg
     9901 gctcggcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg
     9961 tggccatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg
    10021 attccccggg gccgggattt accccccacg actccgcccc ctacggcgct ctggatacgg
    10081 ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggt
    10141 aagcttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
    10201 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
    10261 ataag
//
