LOCUS       sequence-331229-       13143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for inducible overexpression of human TET3 short isoform 
            lacking CXXC domain (NM_001366022.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, Friedel 
            RH, Zou H
  TITLE     Circadian clock regulator Bmal1 gates axon regeneration via Tet3 
            epigenetics in mouse sensory neurons.
  JOURNAL   Nat Commun. 2023 Aug 24;14(1):5165. doi: 10.1038/s41467-023-40816-7.
  PUBMED    37620297
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-24"; volume: "14(1):5165. doi"; pages: " 
            10.1038/s41467-023-40816-7"
COMMENT     Sequence Label: pLenti-TRE-FH-TET3 short
FEATURES             Location/Qualifiers
     source          1..13143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    165..282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        304..618
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    348..366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    383..401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    419..437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    455..473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    490..508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    526..544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     591..615
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    696..720
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             744..767
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             780..806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             780..806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    3288..3299
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(5932..5956)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5987..6575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6040..6060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6458..6469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6618..7117
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6653..6676)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7050..7069
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7131..7934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7194..7213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7804..7823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8025..8258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8330..8451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8367..8386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8421..8440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8491..8626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8646..8665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8647..8665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8675..8692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8675..8691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8684..8706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8833..9288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8920..8939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9130..9151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9314..9418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9419..9487,9488..10279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9637..9656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10450..11038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10939..10958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11192..11209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11326..11347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11362..11367,11368..11385,11386..11392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11400..11416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11405..11427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11424..11440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11424..11440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11459..11479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11461..11479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11507..11733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11734..11914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11961..12086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12579..12812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12997..13041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
       61 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
      121 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
      181 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      241 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
      301 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      361 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      421 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      481 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      541 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      601 gtgaaccgtc agatcgcctg gagaattcga gctcggtacc cggggatcca ccggttcgtc
      661 gactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct
      721 ccgcggccgc ccccttcacc atggactaca aggacgacga tgacaagctc gatggaggat
      781 acccctacga cgtgcccgac tacgcccacc agaggaggcc agccgaggtg gaaataaagg
      841 ctggtgaagg agccgggccg tggggacaag gagcggctgt caagacaggc tcagagctca
      901 gcccagttga tggacctgtt ccaggtcaga tggactcagg gccagtgtac catggggact
      961 cacggcagct aagcgcctca ggggtgccgg tcaatggtgc tagagagccc gctggaccca
     1021 gtctgctggg gactgggggt ccttggcggg tagaccaaaa gcccgactgg gaggctgccc
     1081 caggcccagc tcatactgct cgcctggaag atgcccacga tctggtggcc ttttcggctg
     1141 tggccgaagc tgtgtcctct tatggggccc ttagcacccg gctctatgaa accttcaacc
     1201 gtgagatgag tcgtgaggct gggaacaaca gcaggggacc ccggccaggg cctgagggct
     1261 gctctgctgg cagcgaagac cttgacacac tgcagacggc cctggccctc gcgcggcatg
     1321 gtatgaaacc acccaactgc aactgcgatg gcccagaatg ccctgactac ctcgagtggc
     1381 tggaggggaa gatcaagtct gtggtcatgg aaggagggga ggagcggccc aggctcccag
     1441 ggcctctgcc tcctggtgag gccggcctcc cagcaccaag caccaggcca ctcctcagct
     1501 cagaggtgcc ccagatctct ccccaagagg gcctgcccct gtcccagagt gccctgagca
     1561 ttgccaagga aaaaaacatc agcttgcaga ccgccattgc cattgaggcc ctcacacagc
     1621 tctcctctgc cctcccgcag ccttctcatt ccacccccca ggcttcttgc ccccttcctg
     1681 aggccttgtc acctcctgcc cctttcagat ctccccagtc ttacctccgg gctccctcat
     1741 ggcctgtggt tcctcctgaa gagcactcat cttttgctcc tgatagctct gccttccctc
     1801 cagcaactcc tagaactgag ttccctgaag cctggggcac tgacacccct ccagcaacgc
     1861 cccggagctc ctggcccatg cctcgcccaa gccccgatcc catggctgaa ctggagcagt
     1921 tgttgggcag cgccagtgat tacatccagt cagtattcaa gcggcctgag gccctgccta
     1981 ccaagcccaa ggtcaaggtg gaggcaccct cttcctcccc ggccccggcc ccatcccctg
     2041 tacttcagag ggaggctccc acgccatcct cggagcccga cacccaccag aaggcccaga
     2101 ccgccctgca gcagcacctc caccacaagc gcagcctctt cctagaacag gtgcacgaca
     2161 cctccttccc tgctccttca gagccttctg ctcctggctg gtggccccca ccaagttcac
     2221 ctgtcccacg gcttccagac agaccaccca aggagaagaa gaagaagctc ccaacaccag
     2281 ctggaggtcc cgtgggaacg gagaaagctg cccctgggat caagcccagt gtccgaaagc
     2341 ccattcagat caagaagtcc aggccccggg aagcacagcc cctcttccca cctgtccgac
     2401 agattgtcct ggaagggctt aggtccccag cctcccagga agtgcaggct catccaccgg
     2461 cccctctgcc tgcctcacag ggctctgctg tgcccctgcc cccagaacct tctcttgcgc
     2521 tatttgcacc tagtccctcc agggacagcc tgctgccccc tactcaggaa atgaggtccc
     2581 ccagccccat gacagccttg cagccaggct ccactggccc tcttccccct gccgatgaca
     2641 agctggaaga gctcatccgg cagtttgagg ctgaatttgg agatagcttt gggcttcccg
     2701 gccccccttc tgtgcccatt caggaccccg agaaccagca aacatgtctc ccagcccctg
     2761 agagcccctt tgctacccgt tcccccaagc aaatcaagat tgagtcttcg ggggctgtga
     2821 ctgtgctctc aaccacctgc ttccattcag aggagggagg acaggaggcc acacccacca
     2881 aggctgagaa cccactcaca cccaccctca gtggcttctt ggagtcacct cttaagtacc
     2941 tggacacacc caccaagagt ctgctggaca cacctgccaa gagagcccag gccgagttcc
     3001 ccacctgcga ttgcgtcgaa caaatagtgg agaaagatga aggtccatat tatactcact
     3061 tgggatctgg ccccacggtc gcctctatcc gggaactcat ggaggagcgg tatggagaga
     3121 aggggaaagc catccggatc gagaaggtca tctacacggg gaaggaggga aagagctccc
     3181 gcggttgccc cattgcaaag tgggtgatcc gcaggcacac gctggaggag aagctactct
     3241 gcctggtgcg gcaccgggca ggccaccact gccagaacgc tgtgatcgtc atcctcatcc
     3301 tggcctggga gggcattccc cgtagcctcg gagacaccct ctaccaggag ctcaccgaca
     3361 ccctccggaa gtatgggaac cccaccagcc ggagatgcgg cctcaacgat gaccggacct
     3421 gcgcttgcca aggcaaagac cccaacacct gtggtgcctc cttctccttt ggttgttcct
     3481 ggagcatgta cttcaacggc tgcaagtatg ctcggagcaa gacacctcgc aagttccgcc
     3541 tcgcagggga caatcccaaa gaggaagaag tgctccggaa gagtttccag gacctggcca
     3601 ccgaagtcgc tcccctgtac aagcgactgg cccctcaggc ctatcagaac caggtgacca
     3661 acgaggaaat agcgattgac tgccgtctgg ggctgaagga aggacggccc ttcgcggggg
     3721 tcacggcctg catggacttc tgtgcccacg cccacaagga ccagcataac ctctacaatg
     3781 ggtgcaccgt ggtctgcacc ctgaccaagg aagacaatcg ctgcgtgggc aagattcccg
     3841 aggatgagca gctgcatgtt ctccccctgt acaagatggc caacacggat gagtttggta
     3901 gcgaggagaa ccagaatgca aaggtgggca gcggagccat ccaggtgctc accgccttcc
     3961 cccgcgaggt ccgacgcctg cccgagcctg ccaagtcctg ccgccagcgg cagctggaag
     4021 ccagaaaggc agcagccgag aagaagaaga ttcagaagga gaagctgagc actccggaga
     4081 agatcaagca ggaggccctg gagctggcgg gcattacgtc ggacccaggc ctgtctctga
     4141 agggtggatt gtcccagcaa ggcctgaagc cctccctcaa ggtggagccg cagaaccact
     4201 tcagctcctt caagtacagc ggcaacgcgg tggtggagag ctactcggtg ctgggcaact
     4261 gccggccctc cgacccttac agcatgaaca gcgtgtactc ctaccactcc tactatgcac
     4321 agcccagcct gacctccgtc aatggcttcc actccaagta cgctctcccg tcttttagct
     4381 actatggctt tccatccagc aaccccgtct tcccctctca gttcctgggt cctggtgcct
     4441 gggggcatag tggcagcagt ggcagttttg agaagaagcc agacctccac gctctgcaca
     4501 acagcctgag cccggcctac ggtggtgctg agtttgccga gctgcccagc caggctgttc
     4561 ccacagacgc ccaccacccc actcctcacc accagcagcc tgcgtaccca ggccccaagg
     4621 agtatctgct tcccaaggcc cccctactcc actcagtgtc cagggacccc tccccctttg
     4681 cccagagctc caactgctac aacagatcca tcaagcaaga gccagtagac ccgctgaccc
     4741 aggctgagcc tgtgcccaga gacgctggca agatgggcaa gacacctctg tccgaggtgt
     4801 ctcagaatgg aggacccagt cacctttggg gacagtactc aggaggccca agcatgtccc
     4861 ccaagaggac taacggtgtg ggtggcagct ggggtgtgtt ctcgtctggg gagagtcctg
     4921 ccatcgtccc tgacaagctc agttcctttg gggccagctg cctggcccct tcccacttca
     4981 cagatggcca gtgggggctg ttccccggtg aggggcagca ggcagcttcc cactctggag
     5041 gacggctgcg aggcaaaccg tggagcccct gcaagtttgg gaacagcacc tcggccttgg
     5101 ctgggcccag cctgactgag aagccgtggg cgctgggggc aggggatttc aactcggccc
     5161 tgaaaggtag tcctgggttc caagacaagc tgtggaaccc catgaaagga gaggagggca
     5221 ggattccagc cgcaggggcc agccagctgg acagggcctg gcagtccttt ggtctgcccc
     5281 tgggatccag cgagaagctg tttggggctc tgaagtcaga ggagaagctg tgggacccct
     5341 tcagcctgga ggaggggccg gctgaggagc cccccagcaa gggagcggtg aaggaggaga
     5401 agggcggtgg tggtgcggag gaggaagagg aggagctgtg gtcggacagt gaacacaact
     5461 tcctggacga gaacatcggc ggcgtggccg tggccccagc ccacggctcc atcctcatcg
     5521 agtgtgcccg gcgggagctg cacgccacca cgccgcttaa gaagcccaac cgctgccacc
     5581 ccacccgcat ctcgctggtc ttctaccagc acaagaacct caaccagccc aaccacgggc
     5641 tggccctctg ggaagccaag atgaagcagc tggcggagag ggcacgggca cggcaggagg
     5701 aggctgcccg gctgggcctg ggccagcagg aggccaagct ctacgggaag aagcgcaagt
     5761 gggggggcac tgtggttgct gagccccagc agaaagagaa gaagggggtc gtccccaccc
     5821 ggcaggcact ggctgtgccc acagactcgg cggtcaccgt gtcctcctat gcctacacga
     5881 aggtcactgg cccctacagc cgctggatct agtctaaggg tgggcgcgcc gacccagctt
     5941 tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgacaatc aacctctgga
     6001 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6061 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6121 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6181 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6241 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6301 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6361 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     6421 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6481 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6541 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggact
     6601 agtgatctaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     6661 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     6721 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     6781 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     6841 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     6901 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     6961 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     7021 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     7081 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg
     7141 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7201 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7261 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7321 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7381 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7441 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7501 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7561 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7621 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     7681 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7741 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7801 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7861 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7921 acgagttctt ctgaggggat ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa
     7981 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     8041 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8101 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8161 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8221 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8281 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8461 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     8641 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     8701 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8761 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8821 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8881 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8941 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9001 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9061 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9121 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9181 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9241 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     9301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9661 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     9721 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9781 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9841 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9901 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9961 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10021 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10081 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10141 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10201 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10261 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10561 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    10621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11221 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11281 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11341 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11401 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
    11461 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
    11521 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
    11581 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
    11641 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
    11701 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
    11761 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11821 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11881 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    11941 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    12001 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    12061 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    12121 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    12181 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    12241 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    12301 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    12361 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    12421 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    12481 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12541 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12601 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12661 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12721 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    12781 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    12841 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    12901 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    12961 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    13021 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    13081 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    13141 tct
//
