LOCUS       sequence-331230-        8814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for inducible knockdown of human ARNTL(BMAL1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, Friedel 
            RH, Zou H
  TITLE     Circadian clock regulator Bmal1 gates axon regeneration via Tet3 
            epigenetics in mouse sensory neurons.
  JOURNAL   Nat Commun. 2023 Aug 24;14(1):5165. doi: 10.1038/s41467-023-40816-7.
  PUBMED    37620297
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-24"; volume: "14(1):5165. doi"; pages: " 
            10.1038/s41467-023-40816-7"
COMMENT     Sequence Label: pLKO-Tet-On-ARNTL-shRNA2
FEATURES             Location/Qualifiers
     source          1..8814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        190..284
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     201..220
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    266..284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    383..500
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        549..1059
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     978..996
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1068..1640
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1648..1667
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1695..1713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1724..2344
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1797..1816)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2379..2952
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2546..2563)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2773..2792
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2972..3571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2972..2991)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3468..3488
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3699..3932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4004..4125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4041..4060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4095..4114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4165..4300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4321..4339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4349..4366)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4349..4365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4358..4380)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4507..4962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4594..4613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4804..4825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4988..5092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5093..5161,5162..5953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5311..5330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6124..6712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6613..6632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6866..6883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7000..7021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7036..7041,7042..7059,7060..7066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7074..7090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7079..7101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7098..7114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7098..7114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7133..7153
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7135..7153
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7181..7407
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7408..7588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7635..7760
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8253..8486
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8671..8715
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
       61 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      121 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
      181 gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatcc
      241 ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg gcacgcgata
      301 gatggaaagt tctcgagact ttccatctat cgcgtgcttt ttaattctcg acctcgagac
      361 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      421 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      481 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
      541 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
      601 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
      661 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
      721 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
      781 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
      841 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
      901 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
      961 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     1021 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatctgtg agtttgggga
     1081 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
     1141 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     1201 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     1261 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     1321 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     1381 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     1441 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     1501 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     1561 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     1621 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1681 ttggcaaaga attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa
     1741 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     1801 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     1861 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     1921 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     1981 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     2041 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     2101 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     2161 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     2221 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     2281 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     2341 gtccgcgtac agcggctccc gggagttcta gggatctgcc cctctccctc ccccccccct
     2401 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     2461 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     2521 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     2581 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     2641 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     2701 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     2761 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     2821 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     2881 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     2941 acacgatgat aaggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     3001 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3061 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3121 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3181 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3241 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3301 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3361 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3421 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3481 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3541 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     3601 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     3661 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3721 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3901 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     3961 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     4021 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4081 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     4141 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4261 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     4321 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4381 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4441 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4501 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4801 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4861 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4921 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4981 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5041 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5101 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5161 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5221 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5281 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5341 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5461 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6841 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6901 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7081 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7141 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     7201 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     7261 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     7321 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     7381 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     7441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7561 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     7621 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     7681 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     7741 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7801 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7861 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7921 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7981 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8041 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8101 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8161 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8221 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8281 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     8341 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     8401 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     8461 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     8521 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     8581 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     8641 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     8701 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8761 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actt
//
