LOCUS       sequence-331246-       11213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSINAPS; single molecule translation reporter containing a FLAG tag,
            Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Wu B, Eliscovich C, Yoon YJ, Singer RH
  TITLE     Translation dynamics of single mRNAs in live cells and neurons.
  JOURNAL   Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084.
            Epub 2016 May 5.
  PUBMED    27313041
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf1084"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1430-5"
COMMENT     Sequence Label: 
            pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
FEATURES             Location/Qualifiers
     source          1..11213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2694..2750
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2766..2822
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2838..2894
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2910..2966
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2982..3038
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3054..3110
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3126..3182
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3198..3254
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3270..3326
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3342..3398
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3414..3470
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3486..3542
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3558..3614
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3630..3686
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3702..3758
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3774..3830
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3846..3902
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3918..3974
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3990..4046
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4062..4118
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4134..4190
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4206..4262
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4350..4406
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             join(4458..4460,4461..4463,4464..5174)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4503..4524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             5181..5864
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNN
                     EPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSG
                     GPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMN
                     ERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAME
                     KCKSRA"
     misc_feature    7603..8191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7656..7676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8074..8085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8266..8499
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8618..8636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8704..8808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8809..8877,8878..9669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9027..9046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9840..10428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10329..10348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10582..10599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10839..10860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10875..10880,10881..10898,10899..10905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10913..10929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10918..10940
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10937..10953
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10937..10953
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11003..11022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gattacaagg
     2641 atgacgacga taagggcgga ccgggtggat ctggaggtgg aggttctgga ggagaagaac
     2701 ttttgagcaa gaattatcat cttgagaacg aagtggctcg tcttaagaaa ggttctggca
     2761 gtggagaaga actgctttca aagaattacc acctggaaaa tgaggtagct agactgaaaa
     2821 aggggagcgg aagtggggag gagttgctga gcaaaaatta tcatttggag aacgaagtag
     2881 cacgactaaa gaaagggtcc ggatcgggtg aggagttact ctcgaaaaat tatcatctcg
     2941 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg agaagagcta ttatctaaaa
     3001 actaccacct cgaaaatgag gtggcacgct taaaaaaggg aagtggcagt ggtgaagagc
     3061 tactatccaa gaattatcat cttgagaacg aggtagcgcg tttgaagaag ggttccggct
     3121 caggagagga actgctctcg aagaactatc atcttgaaaa tgaggtcgct cgattaaaaa
     3181 agggatcggg cagtggtgag gaactacttt caaagaatta ccacctcgaa aacgaagtag
     3241 ctcgattaaa gaaaggttca gggtcgggtg aagaattact gagtaaaaat tatcatctgg
     3301 aaaatgaggt agcgagacta aaaaagggga gtggttctgg cgaagagttg ctatcgaaaa
     3361 attatcatct tgagaacgaa gttgctaggc tcaaaaaggg ctcaggctca ggcgaggagt
     3421 tgctctcgaa aaactaccac ttggaaaatg aggtcgcgag gttgaaaaag gggagcgggt
     3481 cgggcgagga gttattgagc aaaaactatc atttagagaa cgaagtcgcg cgcttaaaga
     3541 aaggctcggg ctcgggcgaa gaactcttat cgaagaacta ccacctcgaa aatgaggtcg
     3601 ccaggttgaa aaagggcagt ggcagcgggg aggaactctt gagcaagaac taccacttgg
     3661 agaatgaggt cgcgagattg aagaaagggt cggggagcgg cgaggaattg ctcagcaaga
     3721 attatcattt ggagaacgaa gtcgccaggc tcaagaaagg ctcggggtcg ggggaggaat
     3781 tgttgagtaa aaactaccac ttggaaaatg aagtcgccag gctcaaaaaa gggagtggga
     3841 gcggcgaaga gttattgagc aaaaattacc acttggagaa cgaagtggca aggctcaaga
     3901 aagggagcgg cagcggggag gagctcttat cgaagaacta ccacttagag aatgaagtcg
     3961 cccgcttgaa gaaaggctcg gggagcgggg aagagctctt gagcaagaac taccacttgg
     4021 aaaatgaggt ggcgcgcttg aagaaaggga gcgggagcgg ggaagagtta ctatctaaga
     4081 attatcatct cgagaacgag gtggctcgac taaagaaggg ctccggcagt ggggaggaac
     4141 tcctgtcgaa gaactatcat cttgaaaatg aggttgcaag acttaaaaag gggtccggat
     4201 caggtgagga actactcagt aagaattacc acctggaaaa cgaagttgca cgtttgaaga
     4261 aaggatcagg atcaggcgaa gaactgctct caaaagatta tcatttggaa aatgaggttg
     4321 cacgtttaaa aaagggaagt ggcagtggtg aggaacttct gtcgaaaaat tatcatctcg
     4381 agaatgaagt agcccgactt aaaaagggtt ctggctcggg tggaggcggt ggatcaggtg
     4441 gaggaggttc actgcagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4501 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg
     4561 agggcgatgc caccaacggc aagctgaccc tgaagttcat cagtactacc ggcaagctgc
     4621 ccgtgccctg gcccaccctc gtgaccaccc tgagccacgg cgtgcaggtg ttcgcccgct
     4681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4741 aggagcgcac catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt
     4801 tcgagggcga caccctggtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg
     4861 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg
     4921 ccgtcaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac gtggaggacg
     4981 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5041 tgctgcccga cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga
     5101 agcgcgatca catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg
     5161 acgagctgta caaggctaga atgggcagtg tcgagctgaa tctgagggag actgagctgt
     5221 gtcttggtct tcccggtgga gatacagtgg ctccggtaac cggaaacaag agagggttct
     5281 cagagacggt tgatctgaag ctaaatctga ataatgagcc tgcaaacaag gaaggatcta
     5341 cgactcatga cgtcgtgact tttgattcca aggagaagag tgcttgtcct aaagatccag
     5401 ccaaacctcc ggccaaggca caagttgtgg gatggccacc ggtgagatca taccggaaga
     5461 acgtgatggt ttcctgccaa aaatcaagcg gtggcccgga ggcggcggcg ttcgtgaagg
     5521 tatcaatgga cggagcaccg tacttgagga aaatcgattt gaggatgtat aaaagctacg
     5581 atgagctttc taatgctttg tccaacatgt tcagctcttt taccatgggc aaacatggag
     5641 gagaagaagg aatgatagac ttcatgaatg agaggaaatt gatggatttg gtgaatagct
     5701 gggactatgt tccctcttat gaagacaaag acggtgattg gatgctcgtc ggcgacgttc
     5761 cttggccaat gttcgtcgat acatgcaagc gtttacgtct catgaaagga tcggatgcca
     5821 ttggtctcgc tccgagggcg atggagaagt gcaagagcag agctgctagc taggcggact
     5881 gttactgagc tgcgttttac accctttctt tgacaaaacc taacttgcgc agaaaaaaaa
     5941 aaaataagag acaacattgg catggctttg tttttttaaa ttttttttaa agtttttttt
     6001 tttttttttt ttttttaagt ttttttgttt tgttttggcg cttttgactc aggatttaaa
     6061 aactggaacg gtgaaggcga cagcagttgg ttggagcaaa catcccccaa agttctacaa
     6121 atgtggctga ggactttgta cattgttttg tttttttttt ttttggtttt gtcttttttt
     6181 aatagtcatt ccaagtatcc atgaaataag tggttacagg aagtccctca ccctcccaaa
     6241 agccaccccc actcctaaga ggaggatggt cgcgtccatg ccctgagtcc accccgggga
     6301 aggtgacaac cggtaaccta caaacgggtg gaggatcacc ccacccgaca cttcacaatc
     6361 aaggggtaca atacacaagg gtggaggaac accccaccct ccagacacat tacacagaaa
     6421 tccaatcaaa cagaagcacc atcagggctt ctgctaccaa atttatctca aaaaactaca
     6481 acaaggaatc accatcaggg attccctgtg caatatacgt caaacgaggg ccacgacggg
     6541 aggacgatca cgcctcccga atatcggcat gtctggcttt cgaattcagt gcgtggagca
     6601 tcagcccacg cagccaatca gagtcgaata caagtcgact ttcgcgaaga gcatcagcct
     6661 tcgcgccatt cttacacaaa ccacactctc ccctacagga acagcatcag cgttcctgcc
     6721 cagtacccaa ctcaagaaaa tttatgtccc catgcagcat cagcgcatgg gccccaagaa
     6781 tacatcccca acaaaatcac atccgagcac caacagggct cggagtgttg tttcttgtcc
     6841 aactggacaa accctccatg gaccatcagg ccatggactc tcaccaacaa gacaaaaact
     6901 actcttctcg aagcagcatc agcgcttcga aacactcgag catacattgt gcctatttct
     6961 tgggtggacg atcacgccac ccatgctctc acgaatttca aaacacggac aaggacgagc
     7021 accaccaggg ctcgtcgttc cacgtccaat acgattactt acctttcggg atcacgatca
     7081 cggatcccgc agctacatca cttccactca ggacattcaa gcatgcacga tcacggcatg
     7141 ctccacaagt ctcaaccaca gaaactacca aatgggttca gcaccagcga acccactcct
     7201 acctcaaacc tcttcccaca aaactggcaa gcaggatcac cgcttgccca ttccaacata
     7261 ccaaatcaaa aacaattact ggtacagcat cagcgtacca gcccacatct ctcactacta
     7321 tcaaaaacca aaccgttcag caacagcgaa cggtacacac ggaaaaatca actggtttac
     7381 aaatacgaaa gacgatcacg ctttcgtcca gcgcaaacta ttacgaaaaa catccgacgg
     7441 gaagagcaac agccttcccg cggcggaaaa cctcacaaaa acacgacaaa cggatgcacg
     7501 aacacggcat ccgccgacaa cccacaaact tacaaccagg caaacggtgc aggatcaccg
     7561 caccgtacat caaacacctc agatctcata tcgatagatc ctaatcaacc tctggattac
     7621 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7681 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7741 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7801 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7861 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7921 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7981 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8041 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8101 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8161 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca
     8221 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8281 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8341 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8401 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8461 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8521 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa
     8581 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     8641 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8701 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8761 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8821 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8881 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8941 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9001 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     9061 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9121 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9181 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9241 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9301 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9361 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9421 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9481 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9541 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9601 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9661 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9721 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9781 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9961 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10501 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10561 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10621 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
    10681 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10741 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
    10801 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10861 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10921 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
    10981 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11041 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11101 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    11161 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
