LOCUS       sequence-331256-        3729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-351c-3X
FEATURES             Location/Qualifiers
     source          1..3729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(200..217)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(200..216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(209..231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(425..444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     544..566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(604..622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        690..794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(795..863,864..1655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1013..1032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1826..2414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2315..2334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2568..2585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2702..2723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2738..2743,2744..2761,2762..2768)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2776..2792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2781..2803
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2800..2816
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2800..2816
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cttttttttt tcatgtgccg gtctcacacg tgcctggaga ctagtaagcg cttctagtgg
       61 cggttaaaac gcggtagaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
      121 ccaattgagc ggcctcggca ccgggatgcg tttttttttt cgcagcggta ccggatcccc
      181 gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      241 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      301 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      361 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      421 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      481 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      541 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      601 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      661 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      781 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      841 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      901 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      961 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1021 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1081 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1141 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1201 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1261 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1321 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1381 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1441 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1501 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1561 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1621 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1681 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1741 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1801 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1861 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1921 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1981 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2041 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2101 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2161 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2221 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2281 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2341 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2401 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2461 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2521 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2581 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2641 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2701 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2761 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2821 attacgccaa gcttggagat ctggtaccgc tgcgtttttt ttttcatgtg ccggtctcac
     2881 acgtgcctgg agactagtaa gcgcttctag tggcggttaa aacgcggtag acagcgcgta
     2941 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
     3001 gcgttttttt tttcatactc gagcatgctt ttttttttca tgtgccggtc tcacacgtgc
     3061 ctggagacta gtaagcgctt ctagtggcgg ttaaaacgcg gtagacagcg cgtacgtgcg
     3121 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggatgcgttt
     3181 tttttttcgc agcggtaccg ctgcgttttt tttttcatgt gccggtctca cacgtgcctg
     3241 gagactagta agcgcttcta gtggcggtta aaacgcggta gacagcgcgt acgtgcgttt
     3301 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga tgcgtttttt
     3361 ttttcatact cgagcatgct tttttttttc atgtgccggt ctcacacgtg cctggagact
     3421 agtaagcgct tctagtggcg gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg
     3481 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggatgcgtt ttttttttcg
     3541 cagcggtacc gctgcgtttt ttttttcatg tgccggtctc acacgtgcct ggagactagt
     3601 aagcgcttct agtggcggtt aaaacgcggt agacagcgcg tacgtgcgtt taagcggtgc
     3661 tagagctgtc tacgaccaat tgagcggcct cggcaccggg atgcgttttt tttttcatac
     3721 tcgagcatg
//
