LOCUS       sequence-331257-        3705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-343c-3X
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(192..209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(192..208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(201..223)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(417..436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     536..558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(596..614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        682..786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(787..855,856..1647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1005..1024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1818..2406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2307..2326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2560..2577
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2694..2715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2730..2735,2736..2753,2754..2760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2768..2784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2773..2795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2792..2808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2792..2808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttttttcat gtgccggtct cacacgtgcc tggagactag taagcgcttc tagtggcggt
       61 taaaacgcgg tagacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa
      121 ttgagcggcc tcggcaccgg gatgcgtttt ttttcagcgg taccggatcc ccgggtaccg
      181 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      241 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      301 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      361 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
      421 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
      481 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      541 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
      601 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      661 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      721 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      781 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      841 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      901 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      961 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1021 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1081 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1141 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1201 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1261 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1321 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1381 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1441 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1501 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1561 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1621 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2641 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2701 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2761 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2821 aagcttggag atctggtacc gctgtttttt ttcatgtgcc ggtctcacac gtgcctggag
     2881 actagtaagc gcttctagtg gcggttaaaa cgcggtagac agcgcgtacg tgcgtttaag
     2941 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggatgc gttttttttt
     3001 tcatactcga gcatgctttt ttttttcatg tgccggtctc acacgtgcct ggagactagt
     3061 aagcgcttct agtggcggtt aaaacgcggt agacagcgcg tacgtgcgtt taagcggtgc
     3121 tagagctgtc tacgaccaat tgagcggcct cggcaccggg atgcgttttt tttcagcggt
     3181 accgctgttt tttttcatgt gccggtctca cacgtgcctg gagactagta agcgcttcta
     3241 gtggcggtta aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct agagctgtct
     3301 acgaccaatt gagcggcctc ggcaccggga tgcgtttttt ttttcatact cgagcatgct
     3361 tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg
     3421 gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
     3481 aattgagcgg cctcggcacc gggatgcgtt ttttttcagc ggtaccgctg ttttttttca
     3541 tgtgccggtc tcacacgtgc ctggagacta gtaagcgctt ctagtggcgg ttaaaacgcg
     3601 gtagacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca attgagcggc
     3661 ctcggcaccg ggatgcgttt tttttttcat actcgagcat gcttt
//
