LOCUS       sequence-331258-        3498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-169ak-4X
FEATURES             Location/Qualifiers
     source          1..3498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(362..378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    386..402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(410..416,417..434,435..440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    455..476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(593..610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(764..1352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(844..863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1523..2314,2315..2383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2146..2165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2384..2488)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2556..2574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2612..2634)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2734..2753
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2947..2969
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2961..2978
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2962..2978
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattgt gaaaaaaaaa
       61 agggtaccag atccgaatcc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa
      121 ttgagcggcc tcggcaccgg gattgtgaaa aaaaaaaggg taccggatct gattccgtac
      181 gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg caccgggatt
      241 gtgaaaaaaa aaagggtacc agatcttttt ttaagcttgg cgtaatcatg gtcatagctg
      301 tttcatcgga tcccgggccc gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat
      361 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      421 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      481 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      541 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      601 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      661 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1201 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1441 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1501 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1561 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1621 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1681 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1741 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     1801 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     1861 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     1921 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     1981 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2041 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2101 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2161 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2221 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2281 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2341 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2401 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2461 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     2521 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2581 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2641 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     2701 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     2761 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     2821 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2881 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2941 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcggtacc ggatccgaat
     3001 ccgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
     3061 gggattgtga aaaaaaaaag ggtaccggat ctgattccgt acgtgcgttt aagcggtgct
     3121 agagctgtct acgaccaatt gagcggcctc ggcaccggga ttgtgaaaaa aaaaagggta
     3181 ccagatccga atccgtacgt gcgtttaagc ggtgctagag ctgtctacga ccaattgagc
     3241 ggcctcggca ccgggattgt gaaaaaaaaa agggtaccgg atctgattcc gtacgtgcgt
     3301 ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gattgtgaaa
     3361 aaaaaaaggg taccagatcc gaatccgtac gtgcgtttaa gcggtgctag agctgtctac
     3421 gaccaattga gcggcctcgg caccgggatt gtgaaaaaaa aaagggtacc ggatctgatt
     3481 ccgtacgtgc gtttaagc
//
