LOCUS       sequence-33126-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Provost E, Rhee J, Leach SD
  TITLE     Viral 2A peptides allow expression of multiple proteins from a 
            single ORF in transgenic zebrafish embryos.
  JOURNAL   Genesis. 2007 Oct . 45(10):625-9.
  PUBMED    17941043
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Oct . 45(10):625-9."
COMMENT     Sequence Label: pEF6V5:eGFP-CAAX-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..167)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(258..279)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     607..626
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat aggaccgggg
      121 ttttcttcca cgtctcctgc ttgctttaac agagagaagt tcgtggctcc ggatcccata
      181 attacacact ttgtctttga cttctttttc ttcttcttgt acagctcgtc catgccgaga
      241 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      301 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      361 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      421 ttgtggcgga tcttgaagtt cgccttgatg ccgttcttct gcttgtcggc catgatatag
      481 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      541 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      601 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      661 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
      721 cactgcacgc cgta
//
