LOCUS       sequence-331264-        9274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The pSOUP helper plasmid needed for replication of all pGreen 
            (Hellens et al., 2000) based vectors in Agrobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA
  TITLE     An Efficient Agrobacterium-Mediated Transformation Protocol for 
            Hexaploid and Tetraploid Wheat.
  JOURNAL   Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58.
  PUBMED    33656289
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Protoc."; date: "2021-03"; volume: "1(3):e58. doi"; pages: " 
            10.1002/cpz1.58"
COMMENT     Sequence Label: pSOUP
FEATURES             Location/Qualifiers
     source          1..9274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(312..330)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(313..330)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(348..364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(348..364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(361..383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    372..388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(396..402,403..420,421..426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    441..462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(709..714,715..731,732..737)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             785..1975
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1137..1156)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2473..3621
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4750..5721)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein of plasmid pSa"
                     /label=RepA
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
     CDS             6368..7018
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(7457..7476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            7699..7808
                     /note="incP origin of transfer"
     CDS             7841..8212
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      8339..9050
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 attcttggca tagtggtcgc gggtgccgtg ctcgtgttcg ggggtgcgat aaacccagcg
       61 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
      121 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
      181 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatcttttat
      241 atagaagata attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt
      361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      421 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      661 acagaatcag gggataacgc aggaaagaac atgaattaat tctcatgttt gacagcttat
      721 catcgattag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
      781 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
      841 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
      901 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
      961 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     1021 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct
     1081 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     1141 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     1201 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     1261 acatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     1321 cctaatgcag gagtcgcata agggagagcg ccgacccatg cccttgagag ccttcaaccc
     1381 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     1441 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     1501 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     1561 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     1621 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     1681 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     1741 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     1801 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta
     1861 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     1921 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     1981 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
     2041 cggagaactg tgaatgcgca aaccaaccct tgatcgggga agaacagtat gtcgagctat
     2101 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     2161 atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc
     2221 gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc
     2281 aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc
     2341 cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca
     2401 ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg
     2461 caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac
     2521 gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc
     2581 cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc
     2641 gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg
     2701 cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga
     2761 actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag
     2821 gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc
     2881 ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc
     2941 tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc
     3001 attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac
     3061 gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag
     3121 ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg
     3181 tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg
     3241 tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac
     3301 cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt
     3361 gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc
     3421 cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc
     3481 ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc
     3541 gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac
     3601 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc
     3661 agcgctttac tggcatttca ggaacaagcg ggcactgctc gacgcacttg cttcgctcag
     3721 tatcgctcgg gacgcacggc gcgctctacg aactgccgat aaacagagga ttaaaattga
     3781 caattgtgat taaggctcag attcgacggc ttggagcggc cgacgtgcag gatttccgcg
     3841 agatccgatt gtcggccctg aagaaagctc cagagatgtt cgggtccgtt tacgagcacg
     3901 aggagaaaaa gcccatggag gcgttcgctg aacggttgcg agatgccgtg gcattcggcg
     3961 cctacatcga cggcgagatc attgggctgt cggtcttcaa acaggaggac ggccccaagg
     4021 acgctcacaa ggcgcatctg tccggcgttt tcgtggagcc cgaacagcga ggccgagggg
     4081 tcgccggtat gctgctgcgg gcgttgccgg cgggtttatt gctcgtgatg atcgtccgac
     4141 agattccaac gggaatctgg tggatgcgca tcttcatcct cggcgcactt aatatttcgc
     4201 tattctggag cttgttgttt atttcggtct accgcctgcc gggcggggtc gcggcgacgg
     4261 taggcgctgt gcagccgctg atggtcgtgt tcatctctgc cgctctgcta ggtagcccga
     4321 tacgattgat ggcggtcctg ggggctattt gcggaactgc gggcgtggcg ctgttggtgt
     4381 tgacaccaaa cgcagcgcta gatcctgtcg gcgtcgcagc gggcctggcg ggggcggttt
     4441 ccatggcgtt cggaaccgtg ctgacccgca agtggcaacc tcccgtgcct ctgctcacct
     4501 ttaccgcctg gcaactggcg gccggaggac ttctgctcgt tccagtagct ttagtgtttg
     4561 atccgccaat cccgatgcct acaggaacca atgttctcgg cctggcgtgg ctcggcctga
     4621 tcggagcggg tttaacctac ttcctttggt tccgggggat ctcgcgactc gaacctacag
     4681 ttgtttcctt actgggcttt ctcagcccca gatcgatcct tgaccaagcc aagcgtagcg
     4741 ttggcctggt caagtcggag gggggccgat gcgagcgccc ttgccgggtg cgcgggtgac
     4801 atgcaggcgt gtggatttga tgcgcaggca ttcgccgtca tcttcgatgc agtcgcttgc
     4861 ctcgggatag acaatcaaca cttcgcgtag gcgctttttg aagttgtatt tgaagctggc
     4921 gagtgctgcc cgctctgccc gctctcgggc cttatcgtcc agttcgggcg agttgcgtgc
     4981 gcggctgcca taggatgagc cgaattgcgc ttgcagggcg acccaaggga tttgcacgaa
     5041 ggggcggccc ttggcccgca acaggaacac gcgataggtc agccacgtgt aaatgtccat
     5101 cgcaagcgga gactgccgca aggcgtgcag gtagtcgatt cggataggaa ccggtgagcg
     5161 ggtgacttcc tcgaagaaat cgcctgtgag ggtgagggtg ctatcccata gcgcccgatc
     5221 ttctggccgc ttgggattcc agaatagaaa agcgcgcttg gcaatgacga cgttctcaat
     5281 gccgaagtca ttgccttgct cgccggcaag cgaaatcatg gatgaaaaca ggcgttgcgc
     5341 ctgattgcga agggtggccg tgtaacggcc atcggtgtgc attccgagcc tttgtagaaa
     5401 ttccgattgc gaccggccaa ggttcaacac ggggtctttc gttcgcacgg cctcggtgca
     5461 tatccaagca agcaaggtgc gcggcataga accgtagggc aggccgatgc tcggcttgcc
     5521 catgatcgac aaggtgacga tgccattggt gcgctcaaag tagctggtct tggggtcggt
     5581 gtggggcatg gtcgcttgca caaggcaacg ggccatgtag ccgactaagc cagcttcgcg
     5641 ggcatcctcc atttcgagcg cgaggctcgt cttgatgatc tcgttgatac gatggccggg
     5701 ggctttgttg ttcttaggca tgttgttccc tccccggcat ggtgatggtt ggtctagtgt
     5761 ttgtgggttt gatgttccgg cgtttgatga acaggcgcaa ggtgtgaggg ctgacgccta
     5821 acaactcggc tgcgcgactt tgcggcaagc caaggttcac gtatgcctgt acttcatcaa
     5881 tacggctgtc cagcttcaag gcgctcgatt tgctgccctt gggtcgcccg agcgtcttgc
     5941 cgcgctctct ggcgacttgt agcgcctcgg tggtacgtgc ctgaatgaaa tgccgctcga
     6001 tcctctagga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat
     6061 ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca
     6121 gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca
     6181 gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga
     6241 tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa
     6301 cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt
     6361 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa
     6421 cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca
     6481 gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc
     6541 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt
     6601 tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg
     6661 catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg
     6721 cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag
     6781 ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg
     6841 cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt
     6901 cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt
     6961 ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc
     7021 aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct
     7081 ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc
     7141 cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc
     7201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7561 gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     7621 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     7681 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     7741 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     7801 ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc
     7861 aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat
     7921 tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca
     7981 gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa
     8041 tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac
     8101 ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga
     8161 cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta
     8221 gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca
     8281 agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc
     8341 gcctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     8401 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     8461 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     8521 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     8581 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     8641 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     8701 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     8761 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     8821 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     8881 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     8941 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
     9001 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt
     9061 ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc
     9121 cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag
     9181 tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga
     9241 cttcatggcg gggccggcaa tttttacctt gggc
//
