LOCUS       sequence-331265-       10784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Smedley MA, Hayta S, Clarke M, Harwood WA
  TITLE     CRISPR-Cas9 Based Genome Editing in Wheat.
  JOURNAL   Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65.
  PUBMED    33687760
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Protoc."; date: "2021-03"; volume: "1(3):e65. doi"; pages: " 
            10.1002/cpz1.65"
COMMENT     Sequence Label: Level 1 P2 OsUbiP:Cas9:NosT
FEATURES             Location/Qualifiers
     source          1..10784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1532..1556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      1648..2279
                     /label=oriV
                     /note="incP origin of replication"
     promoter        2366..2470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2471..2539,2540..3331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2689..2708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3502..4090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3991..4010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4426..5574
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    5730..5754
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(5802..6054)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6070..6090)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6103..10206)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6103..10206)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6103..10206)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 aactcaacct aaagatttta acctaacgag ctattcatct attggaacaa tatcagaaca
       61 atcccatccg aactaagcac aaataagtaa aactaaatct gatcacaaaa ctcaaactat
      121 gtggcaggat caaaacataa ctgtgaacta ctcttattat ccatcattag ggtaatatat
      181 cgaaacaatc cccatctgac acatcattag ggtaatatat cgaaacaatc cccatctgac
      241 acatcattag ggtaatatat cgaaacaatc cccatctgac agaacaatct caaaaccaat
      301 ccatctatta gatcgatata aataacacag atccctacct cgattctcac gaaaccctaa
      361 atcaatgaga gattaagagg attaccctaa aatcgcacga aaccgtaact cgattaagag
      421 gtggatcacc ctaaaatcgc acgaaaccct gagagattga gactaactcg cacgggatcc
      481 tcctacgctt tccaccacga gcatcaaaat cgcgagatta gacgggcacc acgagagatt
      541 cgaggattag gacgaggtga ttgattagtt accttgacga ggcgattaga gaacgcaaag
      601 cttcgctccc cctccccttc ggtcgattcg actcgctatg cgattaatgg agagaaaacc
      661 tctctatata aagcgtgggc aaggcggtgc gaggcagcat ccggtgcggt caccgccacg
      721 cgtacggccc aatggcagca ccacacgtgt ccatgtagtt ggaggcgcga cacgtgtccg
      781 gtcccggtac gaccatccag acggtgcccg gaaccaactc cgcgtcaggt catgccgcct
      841 ctctttgacg aagtcggcat gcctgatcaa gaccgtacgt ttccgatcca acgatcagga
      901 aacatcgtac ttgatctgct ataagctgcg tgtgggcctt aataatcctc tggggaaata
      961 tttgtgggct gaaatgaacg ggatattggg cctagacaaa ccagggccca tgaattttac
     1021 ggcccaatag attgttgaca cctaattggc taagcccaac tcgcgcactc tgaccaaccg
     1081 atcggaagtt cgcgtccacg ccgccctgct cctccgtcgc cgccggctcg gcggctgacc
     1141 cccgcgccgc cgcaccagct gcgcgcgcgc gcgcgcgcgc ccccaccacc gcggcctctc
     1201 cctcccacgc gcagagggca cgggcagagg ccgccgcggc gccgtactcg ccggcgcttc
     1261 gtcggcctcg gcctcgggtc gaggccggtg gcggggcctc gtcagatcgc cccccttcct
     1321 gtgcagtaag tatttcccac ggacccaata tggattcgtt gttctaatcg atagctgcta
     1381 tcagagatgg gttcttctgc gtgatttacc tgttcttcac atcctccaag cttgaattct
     1441 tgcttgtctt cacagagtgg ggcccactgc atccacccca gtacattaaa aacgtccgca
     1501 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     1561 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     1621 catcagtcag atcaggatct cctttgcgac gctcaccggg ctggttgccc tcgccgctgg
     1681 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
     1741 acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg
     1801 aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg
     1861 gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc
     1921 ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg
     1981 gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga
     2041 ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg
     2101 cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt
     2161 ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg
     2221 cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta
     2281 acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa
     2341 aaatggcagc gctggccaat tcgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2401 ttcaaatatg tatccgctca tgagacaata accttgataa atgcttcaat aatattgaaa
     2461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3181 gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcagatc ctagatgtgg
     4141 cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc
     4201 tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc
     4261 aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt
     4321 gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg
     4381 cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
     4441 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
     4501 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
     4561 atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
     4621 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc ttgaacagga ggcggcaggt
     4681 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
     4741 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac
     4801 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
     4861 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
     4921 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
     4981 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
     5041 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
     5101 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
     5161 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
     5221 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
     5281 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata
     5341 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
     5401 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
     5461 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
     5521 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt
     5581 gtggggtcag ttccggctgg gggttcagca gccagcgcct gatctgggga accctgtggt
     5641 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     5701 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     5761 gatagtttaa accacttcgt gcagaagaca atagtagcgt cgatctagta acatagatga
     5821 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     5881 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     5941 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     6001 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg
     6061 acaagctcac accttcctct tcttcttggg gtcagccctg ctgtctccac cgagctgaga
     6121 gaggtcgatt cttgtttcat agagccccgt aattgactga tgaatcagtg tggcgtccag
     6181 gacctccttt gtagaggtgt accgctttct gtctatggtg gtgtcgaagt acttgaaggc
     6241 tgcaggcgcg cccaagttgg tcagagtaaa caagtggata atgttttctg cctgctccct
     6301 gatgggctta tccctgtgct tattgtaagc agaaagcacc ttatcgaggt tagcgtcggc
     6361 gaggatcact cttttggaga attcgcttat ttgctcgatg atctcatcaa ggtagtgttt
     6421 gtgttgttcc acgaacagct gcttctgctc attatcttcg ggagatcctt tgagcttttc
     6481 atagtggctg gccagataca agaaattaac gtatttagag ggcagtgcca gctcgttacc
     6541 tttctgcagc tcgcccgcac tagcgagcat tcgtttccgg ccgttttcaa gctcaaagag
     6601 agagtacttg ggaagcttaa tgatgaggtc ttttttgacc tctttatatc ctttcgcctc
     6661 gagaaagtcg atggggtttt tttcgaagct tgatcgctcc atgattgtga tgcccagcag
     6721 ttccttgacg cttttgagtt ttttagactt ccctttctcc actttggcca caaccagtac
     6781 actgtaagcg actgtaggag aatcgaatcc gccgtatttc ttggggtccc aatctttttt
     6841 gcgtgcgatc agcttgtcgc tgttcctttt cgggaggata ctttccttgg agaagcctcc
     6901 ggtctgtact tcggtctttt taacgatgtt cacctgcggc atggacagga ccttccggac
     6961 tgtcgcgaaa tccctaccct tgtcccacac gatttctcct gtttctccgt ttgtttcgat
     7021 aagtggtcgc ttccgaatct ctccattggc cagtgtaatc tcggtcttga aaaaattcat
     7081 aatattgctg taaaagaagt acttagcggt ggccttgcct atttcctgct cagactttgc
     7141 gatcattttc ctaacatcgt acactttata gtctccgtaa acaaattcag attcaagctt
     7201 gggatatttt ttgataagtg cagtgcctac cactgcattc aggtaggcat catgcgcatg
     7261 gtggtaattg ttgatctctc tcaccttata aaactgaaag tcctttctga aatctgaaac
     7321 cagcttagac ttcagagtaa taactttcac ctctcgaatc agtttgtcat tttcatcgta
     7381 cttggtgttc atgcgtgaat cgagaatttg ggccacgtgc ttggtgatct ggcgtgtctc
     7441 aacaagctgc cttttgatga agccggcttt atccaactca gacaggccac ctcgttcagc
     7501 cttagtcaga ttatcgaact tccgttgtgt gatcagtttg gcgttcagca gctgccgcca
     7561 ataatttttc attttcttga caacttcttc tgaggggacg ttatcactct tccctctatt
     7621 tttatcggat cttgtcaaca ctttattatc aatagaatca tctttgagaa aagactgggg
     7681 cacgatatga tccacgtcgt agtcggagag ccgattgatg tccagttcct gatccacgta
     7741 catgtccctg ccgttctgca ggtagtacag gtagagcttc tcattctgaa gctgggtgtt
     7801 ttcaactggg tgttccttaa ggatttggga ccccagttct tttataccct cttcaatcct
     7861 cttcatcctt tccctactgt tcttctgtcc cttctgggta gtttggttct ctcgggccat
     7921 ctcgataacg atattctcgg gcttatgcct tcccattact ttgacgagtt catccacgac
     7981 cttaacggtc tgcagtattc cctttttgat agctgggcta cctgcaagat tagcgatgtg
     8041 ctcgtggaga ctgtccccct ggccagaaac ttgtgctttc tggatgtcct ccttaaaggt
     8101 gagagagtca tcatggatca actgcatgaa gttccggttg gcaaatccat cggacttaag
     8161 aaaatccagg attgtctttc cactctgctt gtctcggatc ccattgatca gttttcttga
     8221 cagccgcccc catcctgtat atcggcgcct cttgagctgt ttcatgactt tgtcgtcgaa
     8281 gagatgagcg taagttttca agcgttcttc aatcatctcc ctatcttcaa acaacgtaag
     8341 ggtgaggaca atgtcctcaa gaatgtcctc gttctcctca ttgtccagga agtccttgtc
     8401 tttaatgatt ttcaggagat cgtgatacgt tcccagggat gcgttgaagc gatcctccac
     8461 tccgctgatt tcaacagagt cgaaacattc aatctttttg aaataatctt ctttgagctg
     8521 tttcacggta actttccggt tcgtcttgaa gaggaggtcc acgatagctt tcttctgctc
     8581 tccagacagg aatgctggct ttctcatccc ttctgtgacg tatttgacct tggtgagctc
     8641 gttataaact gtgaagtact cgtacagcag agagtgttta ggaagcacct tttcgttagg
     8701 cagattttta tcaaagttag tcatcctttc gatgaaggac tgggcagagg cccccttatc
     8761 cacgacttcc tcgaagttcc agggagtgat agtctcttct gatttgcgag tcatccacgc
     8821 gaatctggaa tttccccggg cgagggggcc tacatagtag ggtatccgaa atgtgaggat
     8881 tttctcaatc ttttccctgt tatctttcaa aaaggggtag aaatcctctt gccgcctgag
     8941 gatagcgtgc agttcgccca ggtgaatctg gtgggggatg cttccattgt cgaaagtgcg
     9001 ctgtttgcgc aacagatctt ctctgttaag ctttaccagc agctcctcgg tgccgtccat
     9061 tttttccaag atgggcttaa taaatttgta aaattcctcc tggcttgctc cgccgtcaat
     9121 gtatccggcg tagccatttt tagactgatc gaagaaaatt tccttgtact tctcaggcag
     9181 ttgctgtctg acaagggcct tcagcaaagt caagtcttgg tggtgctcat catagcgctt
     9241 gatcatacta gcgctcagcg gagctttggt gatctccgtg ttcactcgca gaatatcact
     9301 cagcagaatg gcgtctgaca ggttctttgc cgccaaaaaa aggtctgcgt actggtcgcc
     9361 gatctgggcc agcagattgt cgagatcatc atcgtaggtg tctttgctca gttgaagctt
     9421 ggcatcttcg gccaggtcga agttagattt aaagttgggg gtcagcccga gtgacagggc
     9481 gataagatta ccaaacaggc cgttcttctt ctccccaggg agctgtgcga tgaggttttc
     9541 gagccgccgg gatttggaca gcctagcgct caggattgct ttggcgtcaa ctccggatgc
     9601 gttgatcggg ttctcttcga aaagctgatt gtaagtctga accagttgga taaagagttt
     9661 gtcgacatcg ctgttgtctg ggttcaggtc cccctcgatg aggaagtgtc cccgaaattt
     9721 gatcatatgc gccagcgcga gatagatcaa ccgcaagtca gccttatcag tactgtctac
     9781 aagcttcttc ctcagatgat atatggttgg gtacttttca tggtacgcca cctcgtccac
     9841 gatattgcca aagattgggt ggcgctcgtg ctttttatcc tcctccacca aaaaggactc
     9901 ctccagccta tggaagaaag agtcatccac cttagccatc tcattactaa agatctcctg
     9961 caggtagcag atccgattct ttctgcgggt atatctgcgc cgtgctgttc ttttgagccg
    10021 cgtggcttcg gccgtctccc cggagtcgaa caggagggcg ccaatgaggt tcttctttat
    10081 gctgtggcga tcggtattgc ccagaacttt gaattttttg ctcggcacct tgtactcgtc
    10141 cgtaatgacg gcccagccga cgctgtttgt gccgatatcg agcccaatgg agtacttctt
    10201 gtccatttat ctggatctgg aagaggcaag aaaggattgg aattaacata ggtaaaaaaa
    10261 agcagctgga gaataagttg gcaccagatc ctacgatatc ggtactgtca gctcaaaata
    10321 gatgaacata tcaatcacat catactacac tacaattgga cagcatcatt aatcataaga
    10381 gcgctgctca cagctcacac cacatcaaca agcaacaacc aaaaatatct tcagataata
    10441 acaaaactga cataagtaac tacaggacaa cagatctatg taagaacaca gaataaagcc
    10501 acctagttct agcatctgac agatttgatt tgcacgagta aagcaaataa aacgggtaaa
    10561 aaaacacagt acaacatcag cactacagtt caactaggca aagagccaga tacaatacag
    10621 attaaattga ccttagaacc caagctctac agttcaagta ggtgtacacg atacagatgt
    10681 aattctctaa cccagctcca cacaattcca atccaaatcc aggggaaaaa aactaagaac
    10741 acaattccaa tccaaatcca gaggaaataa actatgtgtc gcct
//
