LOCUS       sequence-33127-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HBH-kanMX6
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             292..309
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      336..523
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(391..415)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacaggg gttcacatca tcaccaccat
       61 catgctggaa aggccggtga aggtgaaatc cctgcccctc ttgctggtac cgtttctaag
      121 atactggtaa aagaaggtga cactgttaaa gctggtcaaa cagttctggt gctggaggct
      181 atgaaaatgg agacagaaat taacgctcct actgacggaa aagttgaaaa ggtgttagtt
      241 aaggaaagag atgctgttca aggtggtcaa ggtctaatca agatcggcgt tcatcatcac
      301 caccatcatt aatgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
      361 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      421 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      481 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
      541 acagggtaat atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
      601 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
      661 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
      721 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
      781 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
      841 ataaaaggtt ag
//
