LOCUS       sequence-33128-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HBH-kanMX6
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..384)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(459..646)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     567..591
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(673..690)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttatcgcgg cctcgaaacg tgagtctttt ccttacccat ggttgtttat gttcggatgt
       61 gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag
      121 tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc
      181 gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct
      241 tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg
      301 ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag
      361 gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagcta
      421 aacagatcta tattaccctg ttatccctag cggatctgcc ggtagaggtg tggtcaataa
      481 gagcgacctc atactatacc tgagaaagca acctgaccta caggaaagag ttactcaaga
      541 ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt
      601 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgg
      661 cgcgcctcat taatgatggt ggtgatgatg aacgccgatc ttgattagac cttgaccacc
      721 ttgaacagca tctctttcct taactaacac cttttcaact tttccgtcag taggagcgtt
      781 aatttctgtc tccattttca tagcctccag caccagaact gtttgaccag ctttaacagt
      841 gtcaccttct tttaccagta tcttagaaac ggtaccagca agaggggcag g
//
