LOCUS       sequence-331280-       10247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Lee SH, Mayr C
  TITLE     Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated 
            Protein Complex Formation.
  JOURNAL   Mol Cell. 2019 May 16;74(4):701-712.e9. doi: 
            10.1016/j.molcel.2019.03.006. Epub 2019 Apr 1.
  PUBMED    30948266
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.03.006"; journalName: "Mol Cell"; date: 
            "2019-05-16- 16"; volume: "74"; issue: "4"; pages: "701-712.e9"
COMMENT     Sequence Label: pcDNA-GFP-BIRC3-LU
FEATURES             Location/Qualifiers
     source          1..10247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     537..557
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     631..650
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        631..649
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(703..705,706..708,709..1419)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(748..769)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1009..1028)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1356..1377
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5606..5623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5612..5836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5882..6310
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6319..6339)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6324..6653
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6504..6639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6566..6585
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6720..7514
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6774..6793)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7384..7403
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7688..7809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7725..7744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7779..7798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7858..7874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7858..7874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7871..7893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7882..7898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7906..7912,7913..7930,7931..7936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7951..7972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8089..8106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8260..8848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8340..8359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9019..9810,9811..9879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9642..9661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9880..9984)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10059..10078)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      421 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      481 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
      601 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
      661 tggctagcgt ttaaacttaa gcttggtacc gagctcggat ccatggtgag caagggcgag
      721 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      781 aagttcagcg tgtctggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      841 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      901 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      961 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1021 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1081 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1141 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc
     1201 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1321 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1381 gccgccggga tcactctcgg catggacgag ctgtacaaga acatagtaga aaacagcata
     1441 ttcttatcaa atttgatgaa aagcgccaac acgtttgaac tgaaatacga cttgtcatgt
     1501 gaactgtacc gaatgtctac gtattccact tttcctgctg gggttcctgt ctcagaaagg
     1561 agtcttgctc gtgctggttt ctattacact ggtgtgaatg acaaggtcaa atgcttctgt
     1621 tgtggcctga tgctggataa ctggaaaaga ggagacagtc ctactgaaaa gcataaaaag
     1681 ttgtatccta gctgcagatt cgttcagagt ctaaattccg ttaacaactt ggaagctacc
     1741 tctcagccta cttttccttc ttcagtaaca aattccacac actcattact tccgggtaca
     1801 gaaaacagtg gatatttccg tggctcttat tcaaactctc catcaaatcc tgtaaactcc
     1861 agagcaaatc aagatttttc tgccttgatg agaagttcct accactgtgc aatgaataac
     1921 gaaaatgcca gattacttac ttttcagaca tggccattga cttttctgtc gccaacagat
     1981 ctggcaaaag caggctttta ctacatagga cctggagaca gagtggcttg ctttgcctgt
     2041 ggtggaaaat tgagcaattg ggaaccgaag gataatgcta tgtcagaaca cctgagacat
     2101 tttcccaaat gcccatttat agaaaatcag cttcaagaca cttcaagata cacagtttct
     2161 aatctgagca tgcagacaca tgcagcccgc tttaaaacat tctttaactg gccctctagt
     2221 gttctagtta atcctgagca gcttgcaagt gcgggttttt attatgtggg taacagtgat
     2281 gatgtcaaat gcttttgctg tgatggtgga ctcaggtgtt gggaatctgg agatgatcca
     2341 tgggttcaac atgccaagtg gtttccaagg tgtgagtact tgataagaat taaaggacag
     2401 gagttcatcc gtcaagttca agccagttac cctcatctac ttgaacagct gctatccaca
     2461 tcagacagcc caggagatga aaatgcagag tcatcaatta tccattttga acctggagaa
     2521 gaccattcag aagatgcaat catgatgaat actcctgtga ttaatgctgc cgtggaaatg
     2581 ggctttagta gaagcctggt aaaacagaca gttcagagaa aaatcctagc aactggagag
     2641 aattatagac tagtcaatga tcttgtgtta gacttactca atgcagaaga tgaaataagg
     2701 gaagaggaga gagaaagagc aactgaggaa aaagaatcaa atgatttatt attaatccgg
     2761 aagaatagaa tggcactttt tcaacatttg acttgtgtaa ttccaatcct ggatagtcta
     2821 ctaactgccg gaattattaa tgaacaagaa catgatgtta ttaaacagaa gacacagacg
     2881 tctttacaag caagagaact gattgatacg attttagtaa aaggaaatat tgcagccact
     2941 gtattcagaa actctctgca agaagctgaa gctgtgttat atgagcattt atttgtgcaa
     3001 caggacataa aatatattcc cacagaagat gtttcagatc taccagtgga agaacaattg
     3061 cggagactac aagaagaaag aacatgtaaa gtgtgtatgg acaaagaagt gtccatagtg
     3121 tttattcctt gtggtcatct agtagtatgc aaagattgtg ctccttcttt aagaaagtgt
     3181 cctatttgta ggagtacaat caagggtaca gttcgtacat ttctttcatg agagctcaga
     3241 agaaccaaaa catcgtctaa actttagaat taatttatta aatgtattat aactttaact
     3301 tttatcctaa tttggtttcc ttaaaatttt tatttattta caactcaaaa aacattgttt
     3361 tgtgtaacat atttatatat gtatctaaac catatgaaca tatatttttt agaaactaag
     3421 agaatgatag gcttttgttc ttatgaacga aaaagaggta gcactacaaa cacaatattc
     3481 aatcaaaatt tcagcattat tgaaattgta agtgaagtaa aacttaagat atttgagtta
     3541 acctttaaga attttaaata ttttggcatt gtactaatac cgggaacatg aagccaggtg
     3601 tggtggtatg tgcctgtagt cccaggctga ggcaagagaa ttacttgagc ccaggagttt
     3661 gaatccatcc tgggcagcat actgagaccc tgcctttaaa aacaaacaga acaaaaacaa
     3721 aacaccaggg acacatttct ctgtcttttt tgatcagtgt cctatacatc gaaggtgtgc
     3781 atatatgttg aatgacattt tagggacatg gtgtttttat aaagaattct gtgagaaaaa
     3841 atttaataaa gcaacaaaaa ttactcttat tcttcattgc tttatttcaa tgacattgga
     3901 tagtttagtc actcccagac tctttccata ccttcttaaa gcctctcaaa tattgaacta
     3961 cagtttatac tccttcccat aagatgcttc ttcattgaca cttgtagaac acggggtcaa
     4021 cacatcataa aatctattat ggaatgcctg agacaagaat caaacagtcc ctttagtaag
     4081 tttgtttatt cacttctcta ttgattcatt caagaagtct catgccagcc ccacctattg
     4141 gaagaaggtc tgagttttat tcttatctct ttggtattaa ttctgaaact tagaaagtac
     4201 actggttagc aatgcttggg accaacaggt tgttctggta aataaatctg tttcatattg
     4261 tcagtgcaac aaaatgtccc cctctgcatt atgttattgg tactcaacac gtccgagtca
     4321 taactctgtc ctttgcttct tatagaggta ttaggtcttc aagagcagaa gtaagactgt
     4381 aatagggaat actcagggga aggcaggcaa aggctagtca tctaaaccag ttctagatgt
     4441 ctgtataggg gcagatggct ctgtaagggc agaagggaaa gaccccttca taagggtcac
     4501 agctgacaat cctataacaa aagacaggtt aacaagagaa aaacttaaca aatttattta
     4561 atcacagatt tacatcaccg gggagccttc gtaatgaaga tccaaaatta caggggaaac
     4621 tgtgcatttt tatgcttagg tttgataatg aatggacagc cctgaagaat agtgattgga
     4681 aaaaaaggat atgatctaat gggaatagac acaggttggg gacccagcaa ggcctgtctg
     4741 ttcagattat tcttggtctc tgtgcagcat tccttcctcc tggatatagg gcagggcctg
     4801 tatgggatgg ggatattata acctgctatc aagcaaggta ggtcagagaa tttatttatg
     4861 gccagctctt acatagttag gtgaggaaag attagagtac tatctttaag atgtaagtct
     4921 ggcattgtgg aaagatggtt ccagtttcta tgacctacct tggggaagag gaattcaagt
     4981 ttctgtggct tgccttcagg gagaatgagg ctgagacagg agggcaggat aacatcagag
     5041 aaaaactttg cttctgaggc cttcactttg ggttttctga gccccaacat ctgctagtgt
     5101 tgtaaagaga acaattaggg accaagtgag gggaggaaag aatccatctc tgcattctga
     5161 tgctgggaga cttatttcct tgaaatgcaa ttgattttgc ctctgctaag aggctctgct
     5221 ggctacccat gtactagcca gtgtcctgca tgggtgctag gctgaattat ttgtaattgt
     5281 gcttaggtga tttgtaactc aggtataggg tatttaaata gtaggcaccc tttttgcacc
     5341 atgtgttttt ttttttatct agttcttgta tactacagat aatatttgaa ctttgtcatc
     5401 tcactgtaaa acttttgttc atttctcatt atggtaataa atagctatta taaccaaccc
     5461 atttattcaa atatgttatt tccctaagtg ttattttgac attttgtttt ggaaaaaata
     5521 aatcaccata gataataatg gtagatcact agtgttctag agcggccgct cgagtctaga
     5581 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5641 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5701 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5761 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5881 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6301 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6541 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6601 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6661 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     6721 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6781 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6841 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6901 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6961 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7021 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7081 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7141 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7201 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     7261 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7321 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7381 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7441 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7501 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7561 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7621 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7681 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7801 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7861 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7921 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8701 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10021 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10081 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10141 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    10201 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcg
//
