LOCUS       sequence-33129-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HBH-hphMX4
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      334..521
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(389..413)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        596..939
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacaggggt tcacatcatc accaccatca
       61 tgctggaaag gccggtgaag gtgaaatccc tgcccctctt gctggtaccg tttctaagat
      121 actggtaaaa gaaggtgaca ctgttaaagc tggtcaaaca gttctggtgc tggaggctat
      181 gaaaatggag acagaaatta acgctcctac tgacggaaaa gttgaaaagg tgttagttaa
      241 ggaaagagat gctgttcaag gtggtcaagg tctaatcaag atcggcgttc atcatcacca
      301 ccatcattaa tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
      361 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      421 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      481 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
      541 agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
      601 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
      661 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
      721 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcggacct gcgagcaggg
      781 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
      841 aaaaggttag natttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
      901 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaaaag cctgaactca
      961 cncgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtct
//
