LOCUS       sequence-33130-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HBH-TRP1
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      334..521
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(389..413)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        557..704
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacaggggt tcacatcatc accaccatca
       61 tgctggaaag gccggtgaag gtgaaatccc tgcccctctt gctggtaccg tttctaagat
      121 actggtaaaa gaaggtgaca ctgttaaagc tggtcaaaca gttctggtgc tggaggctat
      181 gaaaatggag acagaaatta acgctcctac tgacggaaaa gttgaaaagg tgttagttaa
      241 ggaaagagat gctgttcaag gtggtcaagg tctaatcaag atcggcgttc atcatcacca
      301 ccatcattaa tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
      361 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      421 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      481 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
      541 agggtaatat agatctgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa
      601 ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
      661 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
      721 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
      781 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
      841 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
      901 gttcagg
//
