LOCUS       sequence-33131-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HTB-kanMX6
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      404..591
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(459..483)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaacagggg ttcacatcat caccaccatc
       61 atgactacga tatacccaca accgccagcg agaatctgta ctttcaatca gagttaaaaa
      121 ccgccgcact agctcaacat gacgaggctg ctggaaaggc cggtgaaggt gaaatccctg
      181 cccctcttgc tggtaccgtt tctaagatac tggtaaaaga aggtgacact gttaaagctg
      241 gtcaaacagt tctggtgctg gaggctatga aaatggagac agaaattaac gctcctactg
      301 acggaaaagt tgaaaaggtg ttagttaagg aaagagatgc tgttcaaggt ggtcaaggtc
      361 taatcaagat cggcgtttaa tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt
      421 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
      481 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
      541 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
      601 tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg
      661 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcanggg
      721 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
      781 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
      841 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
      901 agagaaatat aaaaggttag natttgccac tgaggttctt ctttcatata cttcctttta
      961 aatcttgcta ggatacagtt ctcacatca
//
