LOCUS       sequence-331313-        1359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cleavable His-tagged PTPIP51_Delta-TPR domain (36-235) in 
            E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pQlinkH-PTPIP51(36-235)
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(85..179)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     protein_bind    201..225
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(845..865)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(865..889)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(902..922)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             935..946
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    965..981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(989..995,996..1012,1013..1018,1019..1033))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    997..1013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1083..1101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggggcttagc tctgaatctc gccagctagc cttaattaac cactccattt gtaaccactc
       61 catttaaatg gtgttgttac ttggattctc accaataaaa aacgcccggc ggcaaccgag
      121 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      181 aagctcagct aattaagctt accactttgt acaagaaagc tgggtcctag gcggccgcct
      241 acaagcctga ggaacctcca gcctccaggg cactggaggc acctgaagct gcctcttcct
      301 ccaagtcaag agaatccttt ctccccatct tcacagtctc acagctcact tcatcttccc
      361 cgtcctcact ttctttgtca gagtcccgct cattgtcaga ctccgcattg gctgttgtgt
      421 aacccccttc actctcagca tctgtgaacg tggctcccga ggaggccgtg aagtagacag
      481 agctggagcc agtggagtca ctcctctccc ggacaaacgg aaaccttcgc cgccgagcca
      541 ctctctggtt ctcttccatg tggcatcgga cctccccaac aatctccccc gcaagccctc
      601 gcaggctgct tctcagcacc tccacctccc gccgcagcgc cacaaggctg gtcagcacaa
      661 agtccaggcg gtccagcacc ttctcctgtc cttcccgtgg aaggctgggc agcactgagg
      721 catctccagc cccacctggg acagcccgca ggagcatcac gtggcgtccg ggatctgaag
      781 tctgcgtata gtccagggag ttgggcaggc tctggctgcg gccatgacgc tgggtccgtt
      841 tggatccctg aaaataaaga ttctcgcctg cttttttgta caaacttgta cctgcagcac
      901 catggtgatg gtgatggtga tgtttcatag ttaatttctc ctctttaatg aattctgtgt
      961 gaaattgtta tccgctcaca attgaatcta ttataattgt tatccgctca caaagcaaat
     1021 aaatttttta tgatttctcg acaaatggtg ttgttaatta agctcgaggt gaagacgaaa
     1081 gggcctcgtg atacgcctat ttttataggt aatgtcatga taataaaggg tttcttagac
     1141 gtcaggtgga cttttcgggg aaatgtgcgc ggaaccccat ttgtttattt tttaaaacat
     1201 tcaaatatgt atccgccctg agacaataac ccggataaag gtttcataaa attgaaaaag
     1261 agaagatgaa tatttaaaat ttccgggtcg cccttttccc tttttttggg tttttgcctc
     1321 cgtttttctc ccccaaaaac ggggaaaaaa aaaagtgaa
//
