LOCUS       sequence-331314-        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. 
            Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pQlinkH-PTPIP51(236-470)
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(47..61,62..67,68..84,85..91)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    67..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    99..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             134..145
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             158..178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    191..215
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             215..235
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(960..984)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1006..1100
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcatttcggc tactcgagct tattaacaac accatttgtc gagaaatcat aaaaaattta
       61 tttgctttgt gagcggataa caattataat agattcaatt gtgagcggat aacaatttca
      121 cacagaattc attaaagagg agaaattaac tatgaaacat caccatcacc atcaccatgg
      181 tgctgcaggt acaagtttgt acaaaaaagc aggcgagaat ctttattttc agggatccga
      241 ggatgtgctg cccctcctgc agcaggccga cgagctgcac aggggtgatg agcaaggcaa
      301 gcgggagggc ttccagctgc tgctcaacaa caagctggtg tatggaagcc ggcaggactt
      361 tctctggcgc ctggcccgag cctacagtga catgtgtgag ctcactgagg aggtgagcga
      421 gaagaagtca tatgccctag atggaaaaga agaagcagag gctgctctgg agaaggggga
      481 tgagagtgct gactgtcacc tgtggtatgc ggtgctttgt ggtcagctgg ctgagcatga
      541 gagcatccag aggcgcatcc agagtggctt tagcttcaag gagcatgtgg acaaagccat
      601 tgctctccag ccagaaaacc ccatggctca ctttcttctt ggcaggtggt gctatcaggt
      661 ctctcacctg agctggctag aaaaaaaaac tgctacagcc ttgcttgaaa gccctctcag
      721 tgccactgtg gaagatgccc tccagagctt cctaaaggct gaagaactac agccaggatt
      781 ttccaaagca ggaagggtat atatttccaa gtgctacaga gaactaggga aaaactctga
      841 agctagatgg tggatgaagt tggccctgga gctgccagat gtcacgaagg aggatttggc
      901 tatccagaag gacctggaag aactggaagt cattttacga gactaggcgg ccgcctagga
      961 cccagctttc ttgtacaaag tggtaagctt aattagctga gcttggactc ctgttgatag
     1021 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     1081 cgttttttat tggtgagaat ccagtaacaa caccatttaa atggagtggt tacaaatgga
     1141 gtggttaatt aaggctagct tggcgagatt ttcaggagct aaggaagcta aatgggaaaa
     1201 aaatcctggg atttccaccg ttgaaattcc caagggatcc taagaaaatt ttggggtttc
     1261 cttccgttgc ccatggacct taaacagacc gttccccggg atttaggcct ttttaaaaac
     1321 caaaaaaaaa aaaaacaa
//
