LOCUS       sequence-331315-        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. 
            Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pQlinkH-PTPIP51(236-470)
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(85..179)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     protein_bind    201..225
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(950..970)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(970..994)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1007..1027)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             1040..1051
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    1070..1086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1094..1100,1101..1117,1118..1123,
                     1124..1138))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1102..1118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gggccttggc tctgaatctc gccagctagc cttaattaac cactccattt gtaaccactc
       61 catttaaatg gtgttgttac ttggattctc accaataaaa aacgcccggc ggcaaccgag
      121 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      181 aagctcagct aattaagctt accactttgt acaagaaagc tgggtcctag gcggccgcct
      241 agtctcgtaa aatgacttcc agttcttcca ggtccttctg gatagccaaa tcctccttcg
      301 tgacatctgg cagctccagg gccaacttca tccaccatct agcttcagag tttttcccta
      361 gttctctgta gcacttggaa atatataccc ttcctgcttt ggaaaatcct ggctgtagtt
      421 cttcagcctt taggaagctc tggagggcat cttccacagt ggcactgaga gggctttcaa
      481 gcaaggctgt agcagttttt ttttctagcc agctcaggtg agagacctga tagcaccacc
      541 tgccaagaag aaagtgagcc atggggtttt ctggctggag agcaatggct ttgtccacat
      601 gctccttgaa gctaaagcca ctctggatgc gcctctggat gctctcatgc tcagccagct
      661 gaccacaaag caccgcatac cacaggtgac agtcagcact ctcatccccc ttctccagag
      721 cagcctctgc ttcttctttt ccatctaggg catatgactt cttctcgctc acctcctcag
      781 tgagctcaca catgtcactg taggctcggg ccaggcgcca gagaaagtcc tgccggcttc
      841 catacaccag cttgttgttg agcagcagct ggaagccctc ccgcttgcct tgctcatcac
      901 ccctgtgcag ctcgtcggcc tgctgcagga ggggcagcac atcctcggat ccctgaaaat
      961 aaagattctc gcctgctttt ttgtacaaac ttgtacctgc agcaccatgg tgatggtgat
     1021 ggtgatgttt catagttaat ttctcctctt taatgaattc tgtgtgaaat tgttatccgc
     1081 tcacaattga atctattata attgttatcc gctcacaaag caaataaatt ttttatgatt
     1141 tctcgacaaa gggggttgtt aattaagctc caggtgaaga caaaagggcc tccggaaacg
     1201 cccatttttt aagggtaagg gccggaaaaa aaagggtttt ttaaaactcc gggggggcct
     1261 ttccggggga aaggggggcg ggaaacccat ttgttttttt ttttaaaaaa attcaaaaat
     1321 gtttccc
//
