LOCUS       sequence-331318-        1351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. 
            Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pQlinkG2-PTPIP51(236-470)
FEATURES             Location/Qualifiers
     source          1..1351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(798..822)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      844..938
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1097..1116)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gacatcatcg gatctgggtg ctgcaggtac aagtttgtac aaaaaagcag gcgagaatct
       61 ttattttcag ggatccgagg atgtgctgcc cctcctgcag caggccgacg agctgcacag
      121 gggtgatgag caaggcaagc gggagggctt ccagctgctg ctcaacaaca agctggtgta
      181 tggaagccgg caggactttc tctggcgcct ggcccgagcc tacagtgaca tgtgtgagct
      241 cactgaggag gtgagcgaga agaagtcata tgccctagat ggaaaagaag aagcagaggc
      301 tgctctggag aagggggatg agagtgctga ctgtcacctg tggtatgcgg tgctttgtgg
      361 tcagctggct gagcatgaga gcatccagag gcgcatccag agtggcttta gcttcaagga
      421 gcatgtggac aaagccattg ctctccagcc agaaaacccc atggctcact ttcttcttgg
      481 caggtggtgc tatcaggtct ctcacctgag ctggctagaa aaaaaaactg ctacagcctt
      541 gcttgaaagc cctctcagtg ccactgtgga agatgccctc cagagcttcc taaaggctga
      601 agaactacag ccaggatttt ccaaagcagg aagggtatat atttccaagt gctacagaga
      661 actagggaaa aactctgaag ctagatggtg gatgaagttg gccctggagc tgccagatgt
      721 cacgaaggag gatttggcta tccagaagga cctggaagaa ctggaagtca ttttacgaga
      781 ctaggcggcc gcctaggacc cagctttctt gtacaaagtg gtaagcttaa ttagctgagc
      841 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      901 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagtaacaac accatttaaa
      961 tggagtggtt acaaatggag tggttaatta aggctagctt ggcgagattt tcaggagcta
     1021 aggaagctaa aatggagaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1081 tcgtaaaaaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1141 tcagctggaa attacggcct ttttaaagac cgaaagaaaa taagcccagt tttatccggc
     1201 ctttttccat tcttgcccgc cgaagaaggc ctcccggaat tccttggcat gaaaaacggg
     1261 aactggggaa ttgggaaagg ttccccttgt taccgttttc tgaacaatga aaattttttc
     1321 ccttggagga aaaccagaga attcggggtt t
//
