LOCUS       sequence-33132-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HTB-hphMX4
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      409..596
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(464..488)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ctcgtacgct gcaggtcgac ggatccccgg gttaattaac aggggttcac atcatcacca
       61 ccatcatgac tacgatatac ccacaaccgc cagcgagaat ctgtactttc aatcagagtt
      121 aaaaaccgcc gcactagctc aacatgacga ggctgctgga aaggccggtg aaggtgaaat
      181 ccctgcccct cttgctggta ccgtttctaa gatactggta aaagaaggtg acactgttaa
      241 agctggtcaa acagttctgg tgctggaggc tatgaaaatg gagacagaaa ttaacgctcc
      301 tactgacgga aaagttgaaa aggtgttagt taaggaaaga gatgctgttc aaggtggtca
      361 aggtctaatc aagatcggcg tttaatgagg cgcgccactt ctaaataagc gaatttctta
      421 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
      481 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
      541 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
      601 tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca
      661 cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
      721 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt
      781 tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgcg
      841 gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcc
//
