LOCUS       sequence-33133-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-HTB-TRP1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      407..594
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(462..486)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        630..777
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cgtacgctgc aggtcgacgg atccccgggt taattaacag gggttcacat catcaccacc
       61 atcatgacta cgatataccc acaaccgcca gcgagaatct gtactttcaa tcagagttaa
      121 aaaccgccgc actagctcaa catgacgagg ctgctggaaa ggccggtgaa ggtgaaatcc
      181 ctgcccctct tgctggtacc gtttctaaga tactggtaaa agaaggtgac actgttaaag
      241 ctggtcaaac agttctggtg ctggaggcta tgaaaatgga gacagaaatt aacgctccta
      301 ctgacggaaa agttgaaaag gtgttagtta aggaaagaga tgctgttcaa ggtggtcaag
      361 gtctaatcaa gatcggcgtt taatgaggcg cgccacttct aaataagcga atttcttatg
      421 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      481 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
      541 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc
      601 cgctagggat aacagggtaa tatagatctg tacaatcttg atccggagct tttctttttt
      661 tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat
      721 tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttgnagtatg
      781 tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc
      841 acagaggccg cagaatgtgc tctagattcc
//
