LOCUS       sequence-33134-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-BIO-kanMX6
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      296..483
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(351..375)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        558..901
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa cgctggaaag gccggtgaag
       61 gtgaaatccc tgcccctctt gctggtaccg tttctaagat actggtaaaa gaaggtgaca
      121 ctgttaaagc tggtcaaaca gttctggtgc tggaggctat gaaaatggag acagaaatta
      181 acgctcctac tgacggaaaa gttgaaaagg tgttagttaa ggaaagagat gctgttcaag
      241 gtggccaagg tctaatcaag atcggcgttt aatgaggcgc gccacttcta aataagcgaa
      301 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
      361 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
      421 gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac
      481 cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc tcgtccccgc
      541 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
      601 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
      661 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
      721 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
      781 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
      841 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
      901 catgggtaag gaaaagactc acgtttcgag gccgcgatta aattcca
//
