LOCUS       sequence-331346-        2973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target plasmid containing PmcPSP1 and attachment site for PmcCAST..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS30: pTarget(PmcCAST)
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     249..266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             834..1493
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(900..919)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1924..1943
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      join(2560..2973,1..132)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
       61 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
      121 agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg
      181 catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga
      241 ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct
      301 gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc
      361 agtgccaaca tagtaagcca gtatacactc cgctagcgct gatgtccggc ggtgcttttg
      421 ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc
      481 actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccga
      541 cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc
      601 tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga
      661 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac tgggcagtac
      721 cagactcgaa ctgatgacat cctgcttgta aggcaggcgc tctaccaact gagctaaccg
      781 ccccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
      841 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      901 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      961 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     1021 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     1081 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     1141 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     1201 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     1261 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1321 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1381 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1441 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     1501 aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc
     1561 ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg
     1621 ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt
     1681 gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat
     1741 aattgacgat atgatcattt attctgcctc ccagagcctg ataaaaacgg ttagcgcttc
     1801 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1861 aacataatgg tgcagggcgc ttgtttcggc gtgggtatgg tggcaggccc gatcttcccc
     1921 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     1981 cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga tcgcgtagtc
     2041 gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag
     2101 tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc
     2161 atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg
     2221 cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc
     2281 attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc
     2341 gcattaaagc ttccgcctca agctgttgga aatcgctgcg tatgtgggcg ctgcccatta
     2401 aagacgtagc ccagcgcgtc ggccgctacg tatcgtagat atatctacgc gtagatatat
     2461 ctacgtttaa cagtggcctt attaaatgac ttctccatga tctacgtatc gtagatacta
     2521 cgtatcgtag atactacgta tattaataag atgatcttct tgagatcgtt ttggtctgcg
     2581 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     2641 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     2701 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     2761 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     2821 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     2881 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     2941 aacagcggaa tgacaccggt aaaccgaaag gca
//
