LOCUS       sequence-331350-        4291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in E coli and perform ligand binding in solution.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Qing R, Tao F, Chatterjee P, Yang G, Han Q, Chung H, Ni J, Suter BP,
            Kubicek J, Maertens B, Schubert T, Blackburn C, Zhang S
  TITLE     Non-full-length Water-Soluble CXCR4(QTY) and CCR5(QTY) Chemokine 
            Receptors: Implication for Overlooked Truncated but Functional 
            Membrane Receptors.
  JOURNAL   iScience. 2020 Oct 28;23(12):101670. doi: 
            10.1016/j.isci.2020.101670. eCollection 2020 Dec 18.
  PUBMED    33376963
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-10-28"; volume: "23(12):101670. doi"; pages: " 
            10.1016/j.isci.2020.101670. eCollection 2020 Dec 18"
COMMENT     Sequence Label: CCR5-SZ218a QTY
FEATURES             Location/Qualifiers
     source          1..4291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(185..214)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             876..898
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(929..948)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(930..948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1457..1648
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1642..1664)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1750..1889
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1805..1824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1904..1921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2075..2663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2155..2174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2834..3625,3626..3694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3457..3476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3695..3799)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3825..4280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3962..3983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4174..4193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttagtcg acaagcttgg
      181 atccgtggtg gtggtggtga tgatggtgat gatgcagacc aacgctaatt tcctgctcgc
      241 cggtgctacg ggtatacacg ctgctcgcac gttccggcgc ttcctgttga aaaatgctgc
      301 agcacttgca gaaacgtttc gcgatgtgct tctgaaagaa aaccagcagg tagttacgga
      361 atttttcgcc cacatacgcg taggtggtcg ggttgatgca gcagtgggtc ataccctggg
      421 tctcggtggt ctgcatcgct tgatccagac ggttgctgct gctgcagttg ttcagaccaa
      481 agaattcttg aaaggtgttc agctgttggg tggtatagta ggtgccgcta tagtaaccgg
      541 tgaaatactg gccggtctgt tgctggcaca tggtgttacc aaagtcccat tgcgccgccg
      601 catagtgcgc ccaaaacggg gtggtttgct gaaagaactg atcgctaatc gcctggtttt
      661 gcaggtagat gtcggtcatg cttttcagac gcttgcagtt gatctgggtt tgggtggttt
      721 gcatgttgcc ggtaaaaccg aaggtatagg tctggctgta ttgcggcggt tgctgacgcg
      781 ccgcaatctg tttaacgttg atcttttggc acggctcgct ggtatagtag ttaatatcat
      841 agatcgggct gctcacttgg tagtccatat gtatatctcc ttcttaaagt taaacaaaat
      901 tatttctaga gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat
      961 cgagatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
     1021 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
     1081 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
     1141 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
     1201 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
     1261 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
     1321 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
     1381 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
     1441 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     1501 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     1561 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     1621 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1681 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1741 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     1801 tatgcggcat cagagcagat tgtactgaga gtgcaccatt gcggtgtgaa ataccgcaca
     1861 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     1921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3121 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     3181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     3841 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3901 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3961 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4021 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4081 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4141 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4201 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4261 tgcgccgcta cagggcgcgt cccattcgcc a
//
