LOCUS       sequence-331351-        4207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in E coli and perform ligand binding in solution.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Qing R, Tao F, Chatterjee P, Yang G, Han Q, Chung H, Ni J, Suter BP,
            Kubicek J, Maertens B, Schubert T, Blackburn C, Zhang S
  TITLE     Non-full-length Water-Soluble CXCR4(QTY) and CCR5(QTY) Chemokine 
            Receptors: Implication for Overlooked Truncated but Functional 
            Membrane Receptors.
  JOURNAL   iScience. 2020 Oct 28;23(12):101670. doi: 
            10.1016/j.isci.2020.101670. eCollection 2020 Dec 18.
  PUBMED    33376963
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-10-28"; volume: "23(12):101670. doi"; pages: " 
            10.1016/j.isci.2020.101670. eCollection 2020 Dec 18"
COMMENT     Sequence Label: CCR5-SZ190b QTY
FEATURES             Location/Qualifiers
     source          1..4207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(185..214)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             792..814
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(845..864)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(846..864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1373..1564
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1558..1580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1666..1805
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1721..1740
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1820..1837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1991..2579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2071..2090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2750..3541,3542..3610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3373..3392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3611..3715)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3741..4196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3878..3899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4090..4109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttagtcg acaagcttgg
      181 atccgtggtg gtggtgatga tggtgatgat ggtgcaggcc aacgctaatt tcctgctcgc
      241 cggtgctacg ggtgtacacg ctgctcgcac gttccggcgc ttcctgttgg aaaatgctgc
      301 agcacttgca aaaacgtttc gcgatgtgct tttgaaagaa aaccagcaga tagttacgga
      361 atttttcgcc cacaaacgcg tagatggtcg ggttggtgca gcagtgggtc ataccctggg
      421 tctcggtaac ttgcatcgcc tggtccagac ggttgctgct gctgcagttg ttcagaccaa
      481 agaattcttg aaaggtgttc tgttgctgca cggtgttata cggcgcccag tacggggtgg
      541 tttgctgaaa gaactgatcg ctaatcgcct ggttttgcag atagatgtcg gtcatgcttt
      601 tcagacgctt gcagttgatc tgggtttggg tggtctgcat gttgccggtg aaaccaaagg
      661 tgaaggtttg gctgtactgc ggcggttgca gacgcgccgc aatttgttta acgttgatct
      721 tctggcacgg ctcgctggta tagtagttaa tatcatagat cgggctgctc acttggtagt
      781 ccatatgtat atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg
      841 gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgggca gcgttgggtc
      901 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
      961 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     1021 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     1081 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     1141 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     1201 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     1261 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     1321 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa
     1381 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
     1441 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     1501 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1561 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1621 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     1681 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     1741 ctgagagtgc accattgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1801 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2401 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3061 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3361 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3601 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3661 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3721 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3781 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3841 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3901 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3961 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4021 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4081 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4141 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     4201 ttcgcca
//
