LOCUS       sequence-33136-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-BIO-TRP1
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      291..478
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(346..370)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        514..661
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacgctg gaaaggccgg tgaaggtgaa
       61 atccctgccc ctcttgctgg taccgtttct aagatactgg taaaagaagg tgacactgtt
      121 aaagctggtc aaacagttct ggtgctggag gctatgaaaa tggagacaga aattaacgct
      181 cctactgacg gaaaagttga aaaggtgtta gttaaggaaa gagatgctgt tcaaggtggc
      241 caaggtctaa tcaagatcgg cgtttaatga ggcgcgccac ttctaaataa gcgaatttct
      301 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
      361 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
      421 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
      481 gatccgctag ggataacagg gtaatataga tctgtacaat cttgatccgg agcttttctt
      541 tttttgccga ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg
      601 gcattggtga ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag
      661 tatgtctgtt attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca
      721 gagcacagag gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg
      781 tgtgcccaat agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt
      841 aaaagcatat aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca
      901 acctaanagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaac
//
