LOCUS       sequence-33137-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-RGS18HIS-kanMX6
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..72
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             46..75
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             49..78
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..81
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             55..84
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             58..87
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             61..90
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             64..93
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             67..96
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      123..310
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(178..202)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        385..728
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(796..815)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacaggggtt cacatcatca ccaccatcat
       61 catcatcacc accatcatca tcatcaccac catcattaat gaggcgcgcc acttctaaat
      121 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
      181 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
      241 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
      301 cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg
      361 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
      421 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
      481 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
      541 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
      601 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
      661 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
      721 aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg
      781 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca tgtgcgacaa
      841 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
      901 gcgttgccaa tgatgttaca gatg
//
