LOCUS       sequence-331371-       10924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and 
            constitutive RFP expression and strong temperature sensitive GFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS
  TITLE     Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E.
            coli Nissle 1917.
  JOURNAL   ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466.
  PUBMED    33301298
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466."
COMMENT     Sequence Label: pM2s2AsR_TS-E10
FEATURES             Location/Qualifiers
     source          1..10924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4276..4305)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4352..4373
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(4453..4521,4522..5313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5324..5382
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(5442..5471)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     promoter        5553..5587
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             5596..5607
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5614..6291
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      6336..6407
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6423..6450
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6457..6477
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      6513..6544
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_RNA        6889..6938
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             6993..7010
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7023..7736
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYKG"
     promoter        7790..7867
                     /gene="lacI"
                     /label=lacI promoter
     CDS             9023..9067
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      9119..9166
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             9338..9355
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9422..9469
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attaaataat gacaatgttg ggttgcagtg aattcttttt gttttgatgt gcaaaaaacc
       61 gacgataatc aaaacaaaca aaaaattaac tatatttgat ggtttgctta aatcagtaaa
      121 gaccaacggc attatgtacg ttgataaaaa agaaagatac tcaccggatt cttttttaca
      181 tgaaacgacc tttaactttc ttgacaccgc accggagtct aagtttttca aaacccatcg
      241 ataccaaatg tatgtataag aacaagttaa aatcaaagcc ccgcagatca ctgacctcaa
      301 tacagaaaat gttaatctgc tatttgaata gtcgagtacg cattgaaatt ttccatccgc
      361 gccagaacac gaagacatgg ccttatctaa aacagaccat acgttatcaa taccagaaaa
      421 atatattgtt atcggtataa ataaaaacaa cattgataag agatacattc taattttcat
      481 ttttgtaaaa tttcctgtac cacgttgatc tacttattcc taaagaaatc cattctccat
      541 ctctaacttt cggccttcca ccaccagagc ttttttttcc acgttgacgc tgaatttcag
      601 aagtatgtgt ttgtttaaca tactcttcaa agccaagctc tgtaaggttc ttacttgtcc
      661 acttagccac acttttagca attcccatga cttcgttctc gtctaaaggt gcggagaact
      721 gcaggttgta ggctttagcg cgttcaatgc aggcttgtag ccattggtca tactgcggcc
      781 agccttggcg gatagcgcgg taagcccact tgcgggtttt atcgaagagg gtgcagttac
      841 ggcctaaacc gtagtccggc aggatttcgc ggtcattggc tgcgccaagg tcgaggtaat
      901 cggctaacca gtcaagggta tagagctctg gctgccagac ggtgatctgc cagtgcaggt
      961 ggttcggatt cttgcaaatt agccctgaat accccacatc tgcgcccaat tttttacgca
     1021 gcgcattctc gatggcggcg gcgtatttaa ggggggcagc tcgaccatcc ggcgcggtac
     1081 gtaccgccgt atgcaaggca tacaacaggt gagcatgtcc gttctcgggg tttttgatgg
     1141 tgagtgtggg cgcaggtgcc cccagatcgg cccaatcaat cgcggctccg gctctgtcca
     1201 cgtcaaagca aagccagtac atggcgtgag gctgattaaa ctggatgtat tttgcgagga
     1261 gagcacgctc tttaccggca atgcgaacac caaactgtaa atcatcggag aagtacggct
     1321 tgtggggtaa ccggtcgtta aaaagcgtta aagcctgatt atccaaggct cccagcctta
     1381 tggcggggct gttgttttgc acgctgcatg tgctaatatc ctttctaggt ttcgacctag
     1441 ccctgaatgt catgtccgct cgccaaagta gagcatgatt tcggggcttt gttttttctg
     1501 ccactaagtt acacctcaac aacggttttt gtcatccccg acaatccgtt attcctgctt
     1561 gttctcgcac ggctttacgc tcatactact tcttgtagat acacttgtca ctacatcaag
     1621 aggtgagatg atggccacga ttaatattcg gatcgatgac gagctgaaaa gccgctctta
     1681 tgccgcactg gaaaagctgg gcgtaacgcc gtccgaggtt ctgcgccaaa cactggaata
     1741 tgtggcccaa agcggacgtt tgccgttcca gcaggttttg ctgaccgagg atgatgccga
     1801 tttgatggct atcgttcggg atcgtctgga aaacccacag gcgggcgtaa aggtgtcact
     1861 ggatgagcta taaccttgaa tttgatcccc gagccctgaa ggaatggcgc aagctcgggg
     1921 atgatgtccg tctgcagttc aagaaaaaac tcgagcaggt tctacaacac ccgcggatcg
     1981 ataaaaatcg cctgcgagag ctgcatgact gctacaaaat caagctccgt gcatccggtt
     2041 atcgcttggt ctatcaggtt cgcgatcaaa ccattacggt attcgtggtg gcggtcggta
     2101 agcgcgagcg ttctgccgct tacgatgcgg ccgataaacg cttataaact catgccgtga
     2161 ccgcgagaat accgctgttc gtgccgcttg gctaattgct caagcggcgc actgttctgt
     2221 ttaagctctc gacttgctgc gccaagccgg tgacttgcat gctcaaggcc tgacaaaccc
     2281 tgtcgttttt cgccaactcc tgcgaggtaa cctcgaacat gcgctgtaag ttggcgtagc
     2341 tgtcctgcca cgcttgctgc tgttgttcgt agtgcctctg taagctctct aatgcgctca
     2401 gaagctgctg ctccatttcg gtcatgaatc tcttcaccct gatagataaa accgcccaga
     2461 atcgattctg tggcgtctga tgaggttatt tggcgctgta cttgatgacc tgacgatgtt
     2521 gagcgttctt gtactcgtcg atcttcttcg ccccctgcgg aaggatcagg taatacacgc
     2581 tcttgttctt ggaatcgtga attatcgata cgccggctcc ggtctggctc tttaagtcct
     2641 gcaggatctg gctctgctcg ctgatttcgt tctggcgttc gaccacgata gtcccgagat
     2701 accaagctac tccaatcaat atcgcaaaca ggatcccact taatgccagg ctgtacagcc
     2761 atgtcattcc gactaagcgg tgtatctgtt ttagctggct gtcgttctct tcttggatag
     2821 cggtctgtat gttccctgag cttaatttca aggcctcggt gatacgtttc tcgtgcttct
     2881 cgaatgcgtt cgcgacgctc gttgcggtag tcttggcttg ctgcttcgat ttgctctcga
     2941 actcccgcgc taaatttaaa atctcgctca tacagcactc cttttaagcg aatattcggg
     3001 ccacctgccg gatcagcaat actgatactg gatttggttt cccgtacgac cgacaatccg
     3061 gcatcggtaa ggtgggaaat caccccttta cgatccgtaa tttctccctg ctcaatcaag
     3121 ctgattagcc ctttggtaat ggcttccgct gcctgctgtt tgttgcgagg aaggtcatta
     3181 gagggggtta atgctcggcg attagcaggg tcattcgggt cgcgtaaccc aagccggtca
     3241 ttggtgaggg tttgccatgc gttaacacga ggccggtcag cccgatcaaa gtaaggttgt
     3301 agccgttttc cgctctgcaa ttcgatgttc gggataacaa aattcaattc aagacgccct
     3361 ttgtcctgat gttgaaccca gaggcaggca tactggtctt tatctagacc ggtcatcaat
     3421 gtctgctccc attcatccat caatcgctgc ttttcgcctt cgggtaaatc actctcctga
     3481 aaagagagca cgccagaggt ataagttcgg gcaaattcgc agccatcaat cagctctttg
     3541 acgtgctcag ggttaccccg taacaccgtc gcttgttcgc gctgacgatc agggcccaga
     3601 aggtaatcga caggaccact cccgccaccg gcaccacgac catgaatctt aacgatcacg
     3661 atgttgctcc agcagttcgg caagatgttg gtcaatgcta ttgagcaccg ctaacaacga
     3721 cacccgttct tgcggcggta agccatgctg attcaagtaa cgggctattt gattgaggtt
     3781 attaccgatc ccgctgacct gacgtaacaa ggtcgggtct acggtaggta gcggacgacg
     3841 ccgagctgta cgcgattcgc ctaagccaac ggctcgtaac cactcggcca aatgcttacg
     3901 gtcacagcgt tcaagtagcc gctgatgctc cgcttcggtg agtctgattt tgatctcttt
     3961 ggtgcgcttt tccatgagaa tccgctgaga aagtttcgca cccaaagtgc gaattttcgc
     4021 agtggatgca aggggtttcg gggggcggcg agccccctga aacagtcaca gacggcacct
     4081 cgaagagggg acgctgtgtg tactccctaa atctagaatg cggtcgagct cgtaaacttg
     4141 gtctgacagc tctagctccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc
     4201 tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg tctaagaaaa ggaatattca
     4261 gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgct
     4321 acgtaattag ttagttagcc cttagtgact cgaattcgcg gccgcttcta gagctaaata
     4381 cattcaaata tctatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4441 aaaaggaaga atatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgccgaagat
     4561 cagttgggtg cacgtgtggg ttacatcgaa ctggacctca acagcggtaa gattcttgag
     4621 agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct gctctgtggc
     4681 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga cggcatgaca
     4801 gtacgcgaat tatgcagcgc tgccataacc atgagtgata acacggcggc caacttactt
     4861 ctgacaacga tcggaggacc gaaggagctt accgcttttt tgcacaacat gggtgatcat
     4921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4981 gacaccacga tgcctgtagc tatggcaaca acgttgcgca aactcttaac tggcgaactt
     5041 cttactctcg cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5161 gagcgtgggt cccgcggtat tattgcagcc ctggggccag atggtaagcc ctcccgtatc
     5221 gtagttatct acacgacggg gagccaggca actatggacg aacgtaatcg ccagatcgct
     5281 gagataggtg cctccctgat taagcattgg taataatact agctccggca aaaaacgggc
     5341 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgtttgccac
     5401 ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag gcccacccgt
     5461 gaaggtgagc cagtgagttg attgctacgt aacaatacgc aaaccgcctc tcgttcctta
     5521 tcatctggcg aatcggaccc acaagagcac tgtttacagc tagctcagtc ctaggtatta
     5581 tgctagctac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
     5641 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
     5701 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     5761 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     5821 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     5881 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
     5941 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     6001 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     6061 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     6121 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     6181 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     6241 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     6301 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     6361 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     6421 ctggctcacc ttcgggtggg cctttctgcg tttatatact agtagcggcc gctgcaggag
     6481 tcactaaggg ttagttagtt agattagcag aaagtcaaaa gcctccgacc ggaggctttt
     6541 gactaaaact tcccttgggg ttatcattgg ggctcactca aaggcggtaa tcagatgcgg
     6601 cgagcggtat cagctcactc aaaggcggta atcagataaa aaaaatcctt agctttcgct
     6661 aaggatgatt tctgctagta cgtacggttt cgaagcttgc tcttcttaag cattactcgc
     6721 atccattctc aggctgtctc gtctcgtctc ctgacctcct gccagcaata gtaagacaac
     6781 acgcaaagtc cgatctcgat cccgcgaaat tttaatttgt ttgttagtta gtttatttgt
     6841 tggtttgttt gtgttataaa ataagcctct agaaataatt ttgtttagct gtcaccggat
     6901 gtgctttccg gtctgatgag tccgtgagga cgaaacagcc tctacaaatt ttgtttaaaa
     6961 ctttaagaag gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc
     7021 caatggtgag caagggcgag gagaccacaa tgggcgtaat caagcccgac atgaagatca
     7081 agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg
     7141 gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc
     7201 ccttctccta cgacattctg accaccgcgt tcagttacgg caacagggcc ttcaccaagt
     7261 accccgacga catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc
     7321 gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg
     7381 aggactcctt catctacgag atacacctca agggcgagaa cttccccccc aacggccccg
     7441 tgatgcagaa ggagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg
     7501 gcgtgctgaa gggcgacgtc aagatgaagc tgctgctgga gggcggcggc caccaccgcg
     7561 ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg
     7621 tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg
     7681 agatcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtacaag ggctgaaagc
     7741 ttgcggccgc ataatgctta agtcgaacag aaagtaatcg tatgagctcg acaccatcga
     7801 atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     7861 ggtgaatgta taagaaggag atatacatat gcgtccggcg acatacgaac cagaacagat
     7921 tattgaagca gggctggccc tgcaggctga aggacggaat atcaccgggt tcgcactacg
     7981 taaccaggtg ggtggcggca atccgacacg tctccgccag atatgggacg aataccaggc
     8041 ttcacagagc acggtcgtca ctgaactcgt tgccgagctg ccagtggaag tggctgaaga
     8101 agtgaaggcc gtctccgccg cgctgtccga acgcatcacc cagctggcga cagaactgaa
     8161 tgacaaggcg gtccgggctg cagaacgccg ggttgcggaa gtcacgcgtg ctgccggtga
     8221 acagaccgca caggcagagc gggagctggc cgacgccgcg cagacagtcg acgacctgga
     8281 agaaaaactg gttgaactgc aggacagata tgacagtttg acgctggcgc tggagtcaga
     8341 acgttcactg cgtcagcagc atgatgtgga gatggcccag ctgaaagagc gtcttgcggc
     8401 cgctgaagag aatacccgtc agcgagagga acggtatcag gagcagagga cagtgctgca
     8461 ggatgcgctt aatgcggagc aggcacagca cataaacacg cgggaagacc agcagaaacg
     8521 actggagcaa atttctgccg aagctaatgc gcgtacagaa gaactgaagt ctgaacgcga
     8581 taaagtcaat actctcctta cccgccttga atcgcaggaa aatgcgctgg cctcagaacg
     8641 tcagcagcat ctggccaccc gcgaaacgct gcagcaacgc ctcgagcagg ccatcgctga
     8701 cacgcaggcg cgcgccggtg agattgcact tgaacgtgac agagtcagca gcctcaccgc
     8761 aaggctggaa tcgcaggaaa aggcctcctc ggagcaactg gtgcgtatgg gcagtgaaat
     8821 agccagtctg acagagcgtt gcacacagct ggaaaaccag cgtgatgatg cccgtctgga
     8881 gacgatgggg gagaaagaaa cggtcgcggc actgcgtggt gaggctgaag ccctgaagcg
     8941 tcagaaccag tcactgatgg cggcgctttc aggcaataaa cagaccggtg gccagaatgc
     9001 gtgaggtacc ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
     9061 ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact
     9121 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga ctcggtacca
     9181 aattttcgaa aaaagacgct gaaaagcgtc ttttttcgtt ttggtccgag ccaactccct
     9241 ttacaacctc actcaagtcc gttagagcca ggatacatag attaccacaa ctccgagccc
     9301 ttccacccgc cggcgagacc tctaggccgc actcgagcac caccaccacc accactgaga
     9361 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     9421 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     9481 aactatatcc ggataaacct tgcttcttcg cggtggctta gtacagcatc tatcgtacat
     9541 cgaggtcgca tcacgcacaa acaaaaagcc ccgcaaaagc agggctgtta tctgatatag
     9601 gttgttttgt ctcacacggc agcggaagag gaatccgaag tggtactggt agtagtattg
     9661 gatgctgctg acgatatttt ccgctttgac ccaaggctta aataatcaat gcctgtaatc
     9721 aacgatctca atacgccttc ggataccata gcgataaacg tatcttgctg gttatggctt
     9781 gcgatgcaaa tcgtagcatc acctttttta tactttaaaa cacctgctaa atatccattt
     9841 tcatctagaa cactcttaag atgttcattt gttattgttt gtagcgtttg ctttgtttcg
     9901 cttcgagcat acgccttagc tagcttccga gaaaaagcat ccgcatcatg actatcttta
     9961 tttactcgct caataaattt gcttaagtca acaaatccct taaaacgagt ggacatattg
    10021 ttaacaaaat cagtggcagc attttttatc catgctttat agccaaaaaa acgctcgaaa
    10081 acattttggt cgtagataaa taccgtatcg ccagcaaaaa caagagatgc cttaccatca
    10141 atagaaatca tatcttgatc tactcgagat agttcttttt tgctaaaccc ataaaaatta
    10201 tttttctttc ttgataagtt ttgaactgga tattgcttct tgtatatggt tatacaattg
    10261 tcgtcattat tcttactcaa aacgaaaatg attgagtcaa cttttgatat tagatccaca
    10321 ctgtcaaaat caacaattgg gatattttca ttgccatcac caccaaaccc agcatcaaac
    10381 acaatgccag tcagaaactt catttgctca ttatcatact gctcagcatc atttaaaaat
    10441 gaaatggtgt tggtgcggtc acttagtgta ttaacatctg atactatcac aacccgattt
    10501 tctaaatcag atatagtttt ctttatcaca ttatcaataa tggactgttt tagctcactg
    10561 tcatttttaa ggatggcaat tttatagcta aaagagtcct tagcacccgc tttaccttta
    10621 tttttaaagt taaagtaagt gtgcaatgta acatcgttaa tatcacaatc aaaatgctta
    10681 tacagttcta aaagctcttg tgctttttct tcattatgct ccaaagcatc aagatctaag
    10741 gcatcgtcac tcatcatcat tcctctatga tttttttcgc gaacgttaaa taatcatcat
    10801 gatttataac tctgataaaa tcattttctt ttattaaatc tttagataaa actatcaact
    10861 caccgtcttg cgttttttcc cttccattag ctaccacact gtaagtaatc ttataggcag
    10921 aaac
//
