LOCUS       sequence-33139-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-RGS18HIS-TRP1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..75
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             49..78
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..81
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             55..84
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             58..87
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             61..90
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             64..93
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             67..96
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             70..99
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      126..313
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(181..205)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        349..496
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacaggg gttcacatca tcaccaccat
       61 catcatcatc accaccatca tcatcatcac caccatcatt aatgaggcgc gccacttcta
      121 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
      181 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
      241 actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc
      301 acacctctac cggcagatcc gctagggata acagggtaat atagatctgt acaatcttga
      361 tccggagctt ttcttttttt gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga
      421 gggccaagag ggagggcatt ggtgactatt gagcacgtga gtatacgtga ttaagcacac
      481 aaaggcagct tggagtatgt ctgttattaa tttcacaggt agttctggtc cattggtgaa
      541 agtttgcggc ttgcagagca cagaggccgc agaatgtgct ctagattccg atgctgactt
      601 gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa
      661 aatttcaagt cttgtaaaag catataaaaa tagttcaggc actccgaaat acttggttgg
      721 cgtgtttcgt aatcaaccta aggaggatgt tttggctctg gtcaatgatt acggcattga
      781 tatcgtccaa ctgcatggag atgagtcgtg
//
