LOCUS       sequence-33140-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-HBH
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(56..243)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     164..188
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(270..287)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(516..533)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(586..609)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ctcgtacgct gcaggtcgac ggatctatat taccctgtta tccctagcgg atctgccggt
       61 agaggtgtgg tcaataagag cgacctcata ctatacctga gaaagcaacc tgacctacag
      121 gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt
      181 gtatacactt atttttttta taacttattt aataataaaa atcataaatc ataagaaatt
      241 cgcttattta gaagtggcgc gccggctcca tgatggtggt gatgatgaac gccgatcttg
      301 attagacctt gaccaccttg aacagcatct ctttccttaa ctaacacctt ttcaactttt
      361 ccgtcagtag gagcgttaat ttctgtctcc attttcatag cctccagcac cagaactgtt
      421 tgaccagctt taacagtgtc accttctttt accagtatct tagaaacggt accagcaaga
      481 ggggcaggga tttcaccttc accggccttt ccagcatgat ggtggtgatg atgtgaaccc
      541 ctcatgttaa ttaaagacat tttgagatcc gggttttttc tccttgacgt taaagtatag
      601 aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa
      661 ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt
      721 taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact
      781 aatcgcatta tcatcctatg gttgttaatt tgattcgttc atttgaaggt tgtggggcca
      841 ggttactgcc aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg
//
