LOCUS       sequence-331403-        9096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of phzAG operon for phenazine-1-carboxylic 
            acid production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9096)
  TITLE     Bioelectronic sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSB3K3-101-phzAG
FEATURES             Location/Qualifiers
     source          1..9096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      549..620
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      636..663
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    670..690
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(830..1645)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(830..1645)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1553..1572
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1637..1656
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(2240..2784)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2319..2338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3252..3270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3272..3293
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        3294..3328
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
ORIGIN
        1 cgcccgccgc tacggcgtgc gcgagccgcg ggcactggcc ctggccacgg tcgacgggca
       61 gggccggccg tcgacgcgga tcgtggtgat cgccgagctg ggcgagcgcg gggtggtgtt
      121 cgccacccac gccgatagcc agaagggccg cgaactggcg cagaacccct gggcctcggg
      181 ggtgctgtac tggcgcgaga gcagccagca gatcatcctc aacggccgcg ccgaacgcct
      241 gcccgacgag cgcgccgacg cccagtggct gagccgcccg taccagaccc acccgatgtc
      301 gatcgcctcg cggcagagcg aaaccctcgc cgacatccac gccctgcgcg ccgaagcccg
      361 gcgcctggcg gaaaccgacg gcccgctgcc gcgcccgccg ggctactgcc tgttcgaact
      421 gtgcctggag tcggtggagt tctggggcaa cggcaccgag cggctgcacg agcgcctgcg
      481 ctacgaccgc gacgaaggag gctggaagca ccgctacctg caaccgtgat aatactagag
      541 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      601 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      661 gcgtttatat actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg
      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatctc gagtcccgtc
      781 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
      841 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcgata ccatattttt
      901 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      961 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1021 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1081 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1141 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1201 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1261 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     1321 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     1381 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1441 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     1501 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     1561 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1621 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1681 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1741 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     1801 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     1861 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     1921 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctagc
     1981 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     2041 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     2101 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     2161 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     2221 gggccgcggc aaagccgttt ttccataggc tccgccccct gacaagcatc acgaaatctg
     2281 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2341 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     2401 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2461 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     2521 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     2581 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     2641 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     2701 cagagaacct tcaaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     2761 acgcgcagac caaaacgatc tcaagaagat catcttatta aggggtctga cgctcagtgg
     2821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3181 tccatccagt ctattccatg gtgccacctg acgtctaaga aaccattatt atcatgacat
     3241 taacctataa aaataggcgt atcacgaggc agaattcgcg gccgcttcta gagtttacag
     3301 ctagctcagt cctaggtatt atgctagcta ctagaggagc cctctcggag gcggcgcatg
     3361 aacggtcagc ggtacaggga aacacccctc gacatcgagc gtctgcggcg cctgaatcgc
     3421 gccacggtgg agcgctacat ggcaatgaag ggggccgaac ggttacagcg gcacagcctg
     3481 ttcgtcgagg acggctgcgc cggcaactgg accacggaaa gcggcgaacc cctggttttc
     3541 cggggccatg agagcctcag gcggctcgcc gagtggctcg agcgctgctt ccccgactgg
     3601 gagtggcaca acgtgcggat cttcgagacc gaggatccga accacttctg ggtcgagtgc
     3661 gacgggcgcg gcaaggcgct ggtcccgggg tatccgcagg gctattgcga gaaccactac
     3721 atccattcct tcgaactcga gaacggccgg ataaaacgta atcgcgagtt catgaacccg
     3781 atacagaaac tgcgtgcatt gggaatagcc gttccgcaaa taaaacgtga cggtattccc
     3841 acttgaatga catctaccgc caaggagcat tgacgatgcc tgatacgaca aatccaatcg
     3901 gtttcaccga tgccaacgaa cttcgcgaaa agaatcgcgc caccgtcgag aagtacatga
     3961 ataccaaagg ccaggatcgc ctgcgccgcc atgaactttt cgtcgaggac ggctgtggcg
     4021 gtttatggac caccgatacc ggctcgccca tcgtcattcg tggcaaagac aaacttgcgg
     4081 aacatgccgt ctggtcattg aagtgctttc ccgattggga gtggtacaac ataaatatat
     4141 tcggaaccga cgaccccaat catttctggg tcgaatgcga cggtcacggc aagattcttt
     4201 tccccggata tcccgaagga tattacgaaa accacttcct gcattccttc gaacttgaag
     4261 acggcaagat caagcgcaac cgcgaattta tgaacgtctt ccagcaattg cgcgccttga
     4321 gcattccggt cccgcagatc aaacgcgaag gcattcccac ctgaggccat cctggaaggg
     4381 gtgaactatg gacgatctat tgcaacgcgt acggcgctgc gaagcgttgc agcaacccga
     4441 atggggcgat ccgtcgcgcc tgcgcgacgt gcaggcgtac ctgcgcggca gtccggcgct
     4501 gatccgcgcc ggcgacatcc tggccctgcg cgcgaccctg gcgcgggtcg cccgcggcga
     4561 ggcgctggtg gtgcagtgcg gcgactgcgc cgaggacatg gacgaccacc atgccgagaa
     4621 cgtggcgcgc aaggccgccg tgctggaact gctggccggc gccctgcgcc tggccggccg
     4681 gcggccggtg atccgcgtcg ggcgcatcgc cgggcagtac gccaagccgc gttccaagcc
     4741 gcacgagcag gtcggcgagc agaccctgcc ggtctatcgc ggcgacatgg tcaacggccg
     4801 cgaggcccat gccgaacagc gccgggccga tccgcagcgg atcctcaagg gctatgcggc
     4861 ggcgcgcaac atcatgcgcc acctgggctg ggacgccgcg tccgggcagg aggcgaatgc
     4921 ctcgccggtc tggaccagcc acgagatgct gctgctcgac tacgagctgt cgatgctgcg
     4981 cgaggacgag cagcgccggg tctatctcgg ttcgacccac tggccgtgga tcggcgagcg
     5041 cacccgccag gtcgacggcg cccatgtggc gctgctggcc gaggtgctca acccggtggc
     5101 ctgcaaggtc ggtccggaga tcggccgcga ccagttgctg gcgctctgcg agcgcctcga
     5161 tccgcgccgc gagccgggac gcctgacgct gatcgcgcgg atgggcgcgc agaaggtcgg
     5221 cgagcgcctg ccgccgctgg tggaggcggt gcgcgcggcc gggcacccgg tgatctggct
     5281 gagcgacccg atgcacggca acaccatcgt cgcgccctgc ggcaacaaga cccgcctggt
     5341 gcgcagcatc gccgaggagg tggcggcgtt ccgcctggcg gtgtccggct ccggcggcgt
     5401 ggccgccggg ctgcacctgg aaaccacccc ggacgacgtc accgagtgcg tcgccgattc
     5461 cagcggcctg caccaggtca gccggcacta caccagcctc tgcgatccgc ggctgaaccc
     5521 ctggcaggcg ctcagcgcgg tgatggcctg gtccggcgca gaagcgatcc cgagcgcaac
     5581 cttccccctg gagaccgtgg catgagcggc attcccgaaa tcaccgccta tcccttgccg
     5641 accgcccagc aactgccggc caacctggcg cgctggagcc tggagccgcg gcgcgcggtg
     5701 ctgctggtgc acgacatgca gcgctacttc ctacgcccgc tgcccgagag cctgcgcgcc
     5761 ggactggtgg ccaacgccgc gcggctgcgc cgctggtgcg tcgagcaggg cgtgcagatc
     5821 gcctacaccg cccagccggg cagcatgacc gaggagcagc gcggtctgct caaggacttc
     5881 tgggggccgg gcatgcgcgc cagtccggcc gaccgcgagg tggtcgagga gctggcgccg
     5941 gggccggacg actggctgct gaccaagtgg cgctacagcg ccttcttcca ctccgacctg
     6001 ctccagcgga tgcgcgccgc cggccgcgac cagttggtgc tgtgcggcgt atacgcccac
     6061 gtcggggtgc tgatctccac ggtcgacgcc tactccaacg acatccagcc gttcctggtg
     6121 gccgacgcca tcgccgactt cagcgaggcg caccaccgca tggccctgga atacgccgcc
     6181 agccgctgcg cgatggtggt caccaccgac gaggtgctgg aatgaacgcc ctgcccacat
     6241 cgctgttgca gcgcctgctc gaacggccgg cgccgttcgc cttgctctac cgcccggaaa
     6301 gcaatggccc gggcctgctc gacgtgatcc gcggcgaggc gctcgaactg cacggcctgg
     6361 ccgacctgcc gctggacgag cccggcccgg gcctgccgcg ccatgacctg ctggcgctga
     6421 ttccctaccg gcagatcgcc gagcgcggct tcgaggcgct cgacgacggc acgccgctgc
     6481 tggcgctgaa ggtcctggag caggaactgc tgccgctgga gcaggccctg gcgctgctgc
     6541 cgaaccaggc gctggaactg agcgaggaag gtttcgacct cgacgacgag gcctacgccg
     6601 aagtggtcgg gcgggtcatc gccgacgaga tcggtcgcgg cgaaggcgcc aacttcgtga
     6661 tcaagcggcg cttccaggcg cgcatcgacg gctacgccac ggccagcgcg ctgagcttct
     6721 tccgccagtt gctgctgcgc gagaagggcg cctactggac cttcatcgtc cacaccggcg
     6781 aacgcaccct ggtcggcgcc tcgcccgagc gacacatcag cgtgcgcgac ggcctggcgg
     6841 tgatgaaccc gatcagcggc acctaccgct acccgccggc cgggccgaac ctggccgagg
     6901 tcatggaatt tctcgacaac cgcaaggaag ccgacgagct gtacatggtg gtggacgagg
     6961 agctgaagat gatggcgcgg atctgcgagg acggcggccg ggtgctcggt ccctacctca
     7021 aggagatggc gcacctggcg cacaccgagt acttcatcga gggccagacc agccgcgacg
     7081 tgcgcgaggt gctgcgcgag acgctgttcg ccccgaccgt caccggcagc ccgctggaaa
     7141 gcgcctgccg ggtgatccgc cgctacgagc cgcagggtcg cggctactac agcggggtgg
     7201 cggcgctgat cggcggtgat ggccagggcg ggcgcacgct ggactcggcg atcctgatcc
     7261 gcaccgccga gatcgagggc gacggccgcc tgcgcatcgg cgtcggctcg accatcgtgc
     7321 gtcactccga tcccctgggc gaggcggcgg aaagccgggc caaggcttcc gggctgatcg
     7381 ccgcgctgaa gagccaggcg ccgcagcgcc tgggctcgca cccgcacgtg gtggcggcgc
     7441 tggccagccg caacgcgccg atcgccgact tctggctgcg cggcgccagc gagcgccagc
     7501 agttgcaggc cgacctgtcc ggacgcgagg tgctgatcgt cgatgccgag gacaccttca
     7561 cctcgatgat cgccaagcag ctcaagtcgc tgggcctgac ggtgaccgtg cgcggcttcc
     7621 aggagccgta ctcgttcgac ggctacgacc tggtgatcat gggccccggc ccaggcaacc
     7681 ccaccgagat cggccagccg aagatcggcc acctgcacct ggccatccgt tcgctgctca
     7741 gcgagcgccg gccgttcctc gccgtgtgcc tgagccacca ggtgctgagc ctgtgcctgg
     7801 gcctggacct gcaacgccgg caagagccca accagggcgt gcagaaacag atcgacctgt
     7861 tcggcgccgc cgagcgcgtc ggcttctaca acaccttcgc tgcgcgcgcc ttgcaggacc
     7921 gcatcgagat ccccgaggtc ggcccgatcg agatcagccg cgaccgcgag accggcgagg
     7981 tccatgccct gcgcgggccg cgcttcgcct ccatgcaatt ccaccccgaa tcggtgctga
     8041 cccgcgaggg tccgcgcatc atcgccgacc tgctgcgtca cgcgctggtc gagcggcggc
     8101 cctgagccag gagagcccat gcacagatat gtcgtgatag acgccttcgc cagcgaaccg
     8161 ctgcaaggca acccggtggc ggtgttcttc gactgcgacg acctcagcgg cgagcgcatg
     8221 cagcgcatgg cgcgggagat gaacctgtcg gagagcacct tcgtgctccg cccgcaacag
     8281 gacggcgacg cgcgcatccg catcttcacc ccggtcaacg aactgccgtt cgccggccac
     8341 ccgctgctgg gcaccgccat cgccctgggc gccgagaccg acaaggacag gctgttcctg
     8401 gagaccagga tgggcaccgt gcccttcgcc ctggaacgcc aggacggcaa ggtggtggcc
     8461 tgtagcatgc agcagccgat ccccacctgg gagcacttca gccgaccggc cgagctgctc
     8521 gccgccctcg gcctgaaggg ctcgaccttt cccatcgagg tctaccgcaa cggcccgcgc
     8581 cacgtcttcg tcggcctgga gagcgtcgcc gcgctgtccg cgctgcaccc cgaccaccgc
     8641 gcgctgtgcg acttcccgga cctggcggtg aactgcttcg ccggcgccgg gcggcactgg
     8701 cgcagccgca tgttctcgcc ggcctacggc gtggtcgagg acgccgccac cggttccgcc
     8761 gccgggccgc tggcgatcca cctggcgcgc caccggcaga ttccctacgg gcagcagatc
     8821 gagatcctcc agggcgtcga gatcggccgg ccgtcgcgca tgtacgcccg cgccgagggc
     8881 gccggcgagc gggtctcggc ggtggaagtg tcgggcaacg gcgcggcctt cgccgaaggc
     8941 cgcgcctacc tgtgaacggg cagaacggag gaacgccgtg atgggcgtca acgcgaacat
     9001 ttccgagtcc ctcaccggga ccatcgaagc ccctttcccg gagttcgagg cgccgccggc
     9061 gaaccccatg gaggtgctgc gcaactggct ggaacg
//
